Fig. 1.

BDNF/TrkB protected NB cells from etoposide-induced cell death through down-regulation of P53. a TB3 and TB8 cells were cultured and seeded into 96-well plate as described in “Materials and methods”, and cell survival analysis was tested by MTS assay. Bars, SD P-value were tested by Student’s t test. **P < 0.01. After treated with BDNF (100 ng/ml) for 1 h, the expression of P-Akt (ser473) was detected by Western blot in TB3 and TB8 cells. b The cell lysates from etoposide (0.5 μg/ml) or BDNF (100 ng/ml) treated TB3 and TB8 cells were used to test the expression of P53 by Western blot. c TB3 and TB8 cells were pre-treated with BDNF (100 ng/ml) for 1 h, followed by treatment with etoposide (0.5 μg/ml) for 8 h, and cells were harvested. The cell lysates were used to test the expression of P53 by Western blot. d P53 siRNA and siRNA control were transfected into TB3 and TB8 cells as described in “Materials and methods”, and the expression of P53 was detected by Western blot (protein level) and RT-PCR (mRNA level). Bars, SD P-value were tested by Student’s t test. *P < 0.05, **P < 0.01. e The cell survival of etoposide-treated P53 siRNA-transfected TB3 or TB8 cells was tested by MTS assay. Bars, SD P-value were tested by Student’s t test. **P < 0.01