Fig. 2.

The expression changes of the BCL-2 family members after treatment with etoposide in NB cells. TB3 and TB8 cells were treated with etoposide (0.5 μg/ml), and then cellswere harvested at different time points, and Western blot was done to detect the expressions of BCL2 family members in protein level (a). b The expressions of PUMA, NOXA, BAX, BAK, and MCL1 in mRNA level (treated with etoposide for 4 h) were tested by real-time PCR representatively. Bars, SD P-value were tested by Student’s t test. **P < 0.01