Fig. 5.

Downregulation of PUMA decreased the cell death induced by etoposide both in TB3 and TB8 cells. PUMA siRNA and siRNA control were transfected into TB3 and TB8 cells as described in “Materials and methods”, and the expression of PUMA was detected by Western blot and RT-PCR (a), and after transfection of PUMA siRNA into etoposide-treated TB3 and TB8 cells, cell survival was tested by MTS assay (b). Bars, SD P-value were tested by Student’s t test. *P < 0.05, **P < 0.01. c After transfection of PUMA siRNA into etoposide-treated TB3 and TB8 cells, real-time evaluation of cell confluence was performed by IncuCyte ZOOM. The points showed three replicates. Bars, SD P-value were tested by Student’s t test. *P < 0.05, **P < 0.01