Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Homo sapiens) |
HeLa cell | ATCC | ATCC: CCL-2; RRID:CVCL_0030 | |
| Cell line (Rattus norvegicus) |
Normal rat kidney (NRK) fibroblast cell |
ATCC | ATCC: CRL-1570; RRID:CVCL_2144 | |
| Antibody | GM130 C-terminus (mouse monoclonal) |
BD Biosciences | BD Biosciences: 610822; RRID:AB_398141 | (1:500) |
| Antibody | Golgin 245 (mouse monoclonal) | BD Biosciences | BD Biosciences: 611280; RRID:AB_398808 | (1:100) |
| Antibody | GGA2 (mouse monoclonal) | BD Biosciences | BD Biosciences: 612612; RRID:AB_399892 | (1:200) |
| Antibody | GS15 (mouse monoclonal) | BD Biosciences | BD Biosciences: 610960; RRID:AB_398273 |
(1:250) |
| Antibody | GS27 (mouse monoclonal) | BD Biosciences | BD Biosciences: 611034; RRID:AB_398347 |
(1:250) |
| Antibody | GS28 (mouse monoclonal) | BD Biosciences | BD Biosciences: 611184; RRID:AB_398718 | (1:250) |
| Antibody | Syntaxin6 (mouse monoclonal) | BD Biosciences | BD Biosciences: 610635; RRID:AB_397965 |
(1:250) |
| Antibody | Vti1a (mouse monoclonal) | BD Biosciences | BD Biosciences: 611220; RRID:AB_398752 | (1:500) |
| Antibody | Myc (mouse monoclonal) | Santa cruz biotechnology | Santa cruz: sc-40; RID:AB_627268 | (1:200) |
| Antibody | CLCB (mouse monoclonal) | Santa cruz biotechnology | Santa cruz: sc-376414; RRID:AB_11149726 | (1:200) |
| Antibody | γCOP (mouse monoclonal) | Santa cruz biotechnology | Santa cruz:sc-393977; RRID:AB_2753138 | (1:200) |
| Antibody | Furin (rabbit polyclonal) | Thermo Fisher Scientific | Thermo Fisher Scientific: PA1062; RRID:AB_2105077 | (1:100) |
| Antibody | CI-M6PR (mouse monoclonal) | Thermo Fisher Scientific | Thermo Fisher Scientific: MA1066; RRID:AB_2264554 | (1:200) |
| Antibody | Alexa Fluor 594 conjugated streptavidin | Thermo Fisher Scientific | Thermo Fisher Scientific: S11227; RRID:AB_2313574 | (1:500) |
| Antibody | βCOP (mouse monoclonal) | Sigma-Aldrich | Sigma-Aldrich: G6160; RRID:AB_477023 | (1:200) |
| Antibody | Flag (mouse monoclonal) | Sigma-Aldrich | Sigma-Aldrich: F3165; RRID:AB_259529 | (1:200) |
| Antibody | GM130 N-terminus (rabbit monoclonal) | Abcam | Abcam: ab52649; RRID:AB_880266 | (1:500) |
| Antibody | Giantin N-terminus (rabbit polyclonal) | BioLegend | Biolegend: 924302; RRID:AB_2565451 | (1:1000) |
| Antibody | Giantin C-terminus (rabbit polyclonal) | this paper | (1:500); rabbit polyclonal; against aa3131–3201 | |
| Antibody | KDEL receptor (mouse monoclonal) | Enzo Life Sciences | Enzo Life Sciences: VAA-PT048D; RRID:AB_1083549 | (1:250) |
| Antibody | GALNT1 | Other | (1:10); H Clausen lab (University of Copenhagen) | |
| Antibody | GALNT2 | Other | (1:10); H Clausen lab (University of Copenhagen) |
|
| Antibody | Arl1 (rabbit polyclonal) | PMID: 11792819 | (1:100) | |
| Antibody | Golgin97 (rabbit polyclonal) | PMID: 12972563 | (1:1000) | |
| Recombinant DNA reagent |
pDMyc-neo-N1 | this paper | See Supplementary file 3 | |
| Recombinant DNA reagent | pDMyc-Neo | PMID: 12972563 | ||
| Recombinant DNA reagent |
pGEB | PMID: 11792819 | ||
| Recombinant DNA reagent | pA2E-N1 | PMID: 27369768 | ||
| Recombinant DNA reagent | pmCherry-C2 | this paper | SeeSupplementary file 3 | |
| Recombinant DNA reagent | Streptavidin-His | PMID: 16554831 | RRID:Addgene_20860 | Addgene plasmid #20860 |
| Recombinant DNA reagent | Strep-Ii_VSVG-SBP-EGFP | PMID: 22406856 | RRID:Addgene_65300 | Addgene plasmid #65300 |
| Recombinant DNA reagent | ss-Strep-KDEL_ManII-SBP-GFP | PMID: 22406856 | RRID:Addgene_65252 | Addgene plasmid #65252 |
| Recombinant DNA reagent | ss-Strep-KDEL_ss-SBP-mCherry-GPI | PMID: 22406856 | RRID:Addgene_65295 | Addgene plasmid #65295 |
| Recombinant DNA reagent | TPST1-GFP | PMID: 18522538 | RRID:Addgene_66617 | Addgene plasmid #66617 |
| Recombinant DNA reagent | TPST2-GFP | PMID: 18522538 | RRID:Addgene_66618 | Addgene plasmid #66618 |
| Recombinant DNA reagent | pmScarlet-Giantin-C129 | PMID: 27869816 | RRID:Addgene_85048 | Addgene plasmid #85048 |
| Recombinant DNA reagent | li-Strep_ss-SBP-GFP | this paper | RUSH reporter of soluble SBP-GFP | |
| Recombinant DNA reagent | Strep-Ii_VSVG-SBP-Flag | this paper | RUSH reporter of VSVG-SBP-Flag | |
| Recombinant DNA reagent | ss-Strep-KDEL_ss-SBP-GFP-E-cadherin | PMID: 22406856 | RUSH reporter of SBP-GFP-E-cadherin; a gift from F. Perez lab (Institut Curie) | |
| Recombinant DNA reagent |
ss-Strep-KDEL_ss-SBP-GFP-CD8a-Furin | PMID: 26764092 | RUSH reporter of SBP-GFP-CD8a-Furin | |
| Recombinant DNA reagent |
ss-Strep-KDEL_ss-SBP-GFP-CD59 | PMID: 26764092 | RUSH reporter of SBP-GFP-CD59 | |
| Recombinant DNA reagent |
ss-Strep-KDEL_ss-SBP-GFP-collagenX | Other | RUSH reporter of SBP-GFP-collagenX; a gift from F Perez lab (Institut Curie) |
|
| Recombinant DNA reagent |
Rab1a-GFP | this paper | SeeSupplementary file 3 | |
| Recombinant DNA reagent |
Furin-GFP | this paper | See Supplementary file 3 | |
| Recombinant DNA reagent |
Fuin-Myc | this paper | See Supplementary file 3 | |
| Recombinant DNA reagent |
GFP-GCC185 | this paper | SeeSupplementary file 3 | |
| Recombinant DNA reagent |
GFP-GCC185-mCherry | this paper | SeeSupplementary file 3 | |
| Recombinant DNA reagent |
GFP-ACBD3 | this paper | SeeSupplementary file 3 | |
| Recombinant DNA reagent |
GFP-Rab6 | this paper | SeeSupplementary file 3 | |
| Recombinant DNA reagent |
mCherry-Golgin84 | this paper | SeeSupplementary file 3 | |
| Recombinant DNA reagent |
GFP-GGA1 | this paper | SeeSupplementary file 3 | |
| Recombinant DNA reagent |
mCherry-GM130 | this paper | SeeSupplementary file 3 | |
| Recombinant DNA reagent |
Arf1-GFP | PMID: 16890159 | A gift from FJM van Kuppeveld lab (Utrecht University) |
|
| Recombinant DNA reagent |
Arf4-GFP | Other | A gift from FJM van Kuppeveld lab (Utrecht University) |
|
| Recombinant DNA reagent |
Arf5-GFP | Other | A gift from FJM van Kuppeveld lab (Utrecht University) |
|
| Recombinant DNA reagent |
GFP-ERGIC53 | PMID: 15632110 | A gift from H Hauri lab (University of Basel) | |
| Recombinant DNA reagent |
GFP-GM130 | PMID: 11781572 | A gift from M De Matties lab (Telethon Institute of Genetics and Medicine, Italy) | |
| Recombinant DNA reagent |
GFP-Golgin84 | PMID: 12538640 | A gift from M Lowe lab (University of Manchester) | |
| Recombinant DNA reagent |
GFP-Golgin97 | PMID: 11792819 | A gift from W Hong lab (Institute of Molecular and Cell Biolgoy, Singapore) | |
| Recombinant DNA reagent | GPP130-GFP | PMID: 9201717 | A gift from A Linstedt lab (Carnegie Mellon University) |
|
| Recombinant DNA reagent |
GRASP55-GFP | Other | A gift from Y Zhuang lab (University of Michigan) | |
| Recombinant DNA reagent | GRASP65-GFP | Other | A gift from Y Zhuang lab (University of Michigan) | |
| Recombinant DNA reagent |
DMyc-GCC185 | Other | A gift from W Hong lab (Institute of Molecular and Cell Biolgoy, Singapore) | |
| Recombinant DNA reagent |
Sec23a-mCherry | Other | A gift from W Hong lab (Institute of Molecular and Cell Biolgoy, Singapore) | |
| Recombinant DNA reagent | Sec31a-GFP | PMID: 10788476 | A gift from W Hong lab (Institute of Molecular and Cell Biolgoy, Singapore) | |
| Recombinant DNA reagent |
Vamp4-GFP | PMID: 17327277 | A gift from W Hong lab (Institute of Molecular and Cell Biolgoy, Singapore) | |
| Recombinant DNA reagent | Myc-Sec34 | PMID: 11929878 | A gift from W Hong lab (Institute of Molecular and Cell Biolgoy, Singapore) |
|
| Recombinant DNA reagent |
Myc-Sec13 | PMID: 22609279 | A gift from W Hong lab (Institute of Molecular and Cell Biolgoy, Singapore) |
|
| Recombinant DNA reagent | MGAT1-AcGFP1 | this paper | See Supplementary file 3 | |
| Recombinant DNA reagent | MGAT2-AcGFP1 | this paper | See Supplementary file 3 | |
| Recombinant DNA reagent | MGAT4B-AcGFP1 | this paper | See Supplementary file 3 | |
| Recombinant DNA reagent | ST6Gal1-AcGFP1 | this paper | See Supplementary file 3 | |
| Recombinant DNA reagent | Man1B1-Myc | this paper | See Supplementary file 3 | |
| Recombinant DNA reagent | MGAT1-Myc | this paper | See Supplementary file 3 | |
| Recombinant DNA reagent | MGAT2-Myc | this paper | See Supplementary file 3 | |
| Recombinant DNA reagent | ST6Gal1-Myc | this paper | See Supplementary file 3 | |
| Recombinant DNA reagent | β4GalT3-Myc | this paper | See Supplementary file 3 | |
| Recombinant DNA reagent | GalT-mCherry | PMID: 26764092 | ||
| Recombinant DNA reagent | SLC35C1-Myc | OriGene Technologies Inc. | Cat. No.: RC200101 | |
| Recombinant DNA reagent | β3GalT6-Myc | OriGene Technologies Inc. | Cat. No.: MR204731 | |
| Recombinant DNA reagent | β4GalT7-Myc | OriGene Technologies Inc. | Cat. No.: RC200258 | |
| Recombinant DNA reagent | POMGNT1-Myc | OriGene Technologies Inc. | Cat. No.: RC200176 | |
| Recombinant DNA reagent | FM4-moxGFP | this paper | See Supplementary file 3 | |
| Recombinant DNA reagent | GFP-FM4-CD8a | PMID: 23755362 | A gift from James Rothman lab (Yale University) | |
| Recombinant DNA reagent | GPP130-APEX2-GFP | this paper | See Supplementary file 3 | |
| Recombinant DNA reagent | MGAT2-APEX2-GFP | this paper | See Supplementary file 3 | |
| Recombinant DNA reagent | His-Giantin(3131–3201) | this paper | See Supplementary file 3 | |
| Recombinant DNA reagent | GST-Giantin(3131–3235) | this paper | See Supplementary file 3 | |
| Recombinant DNA reagent | GFP-Nup133-mut | PMID: 27613095 | See Supplementary file 3 | |
| Recombinant DNA reagent | shNup133-1 | PMID: 27613095 | See Supplementary file 3 | |
| Commercial assay or kit | APEX Alexa Fluor 488 Antibody Labeling Kit | Thermo Fisher Scientific | Invitrogen: A10475 | |
| Commercial assay or kit | APEX Alexa Fluor 488 Antibody Labeling Kit | Thermo Fisher Scientific | Invitrogen A10468 | |
| Chemical compound, drug | biotin | IBA | IBA: 21016002 | 40 μM |
| Chemical compound, drug |
biotin phenol | Iris Biotech GmbH | Iris Biotech GmbH: LS3500 | 500 μM |
| Chemical compound, drug | nocodazole | Merck | Merck: 487928 | 33 μM |
| Chemical compound, drug | D/D solubilizer | Clontech | Clontech: 635054 | 1 mM |
| Software, algorithm |
Fiji | PMID: 22743772 | https://fiji.sc/ | |
| Software, algorithm |
Calculation of the LQ | PMID: 26764092; PMID: 28829416 | ||
| Software, algorithm |
Gyradius and intensity normalization.ijm | this paper | To normalize diameters and intensities of en face Golgi mini-stacks | |
| Software, algorithm |
Golgi mini-stack alignment.ijm | this paper | To align normalized en face Golgi mini-stacks | |
| Software, algorithm |
Radial mean intensity profile.ijm | this paper | To measure radial mean intensity of en face averaged Golgi mini-stacks |