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. Author manuscript; available in PMC: 2019 Dec 13.
Published in final edited form as: Cell. 2018 Dec 13;175(7):1756–1768.e17. doi: 10.1016/j.cell.2018.10.025

Figure 6. Integrin inhibitors such as RGD peptide and echistatin block irisin-induced signaling and gene expression.

Figure 6.

(A-B) MLO-Y4 cells were treated as described in Fig. 4E with addition of pretreatment of integrin inhibitors, RGDS peptide or echistatin. Cells were lysed to detect the indicated protein level using immunoblot analysis. (C) MLO-Y4 cells were treated as described in Fig. 1B except with addition of the pretreatment of integrin inhibitors for 10 minutes. (D-E) 8-week-old wild- type C57BL/6J mice were treated as described in Fig. 1C-D except co-injection of 1mg/kg cyclo RGDyK (cRGDyK). Data are represented as mean ± SEM. For C and D, n = 9–12 animals/group. *; p<0.05.