TABLE 2.
Most common amino acid substitutions in CDD β-lactamases
| Enzymea | Amino acid substitutions | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CDD-1 (41)b | S2 | V3 | I5 | G14 | C22 | D74 | N103 | S109 | D165 | R177 | R205 | L214 |
| CDD-1-M1 (6) | Y | |||||||||||
| CDD-2 (312) | K | I | M | Y | E | D | ||||||
| CDD-2-M1 (22) | K | I | M | Y | E | D | C | |||||
| CDD-2-M2 (13) | K | I | M | Y | E | D | I | |||||
| CDD-2-M3 (11) | K | I | M | Y | E | D | Y | |||||
| CDD-2-M4 (24) | K | I | M | Y | E | Y | ||||||
| CDD-2-M5 (9) | K | I | M | Y | E | |||||||
| CDD-2-M6 (16) | K | I | M | D | Y | E | Y | |||||
| CDD-2-M7 (23) | K | I | Y | N | K | |||||||
a
The number of isolates with the given amino acid substitutions is indicated in parentheses. CDD-2-M1 through CDD-2-M7 are mutants of CDD-1 and CDD-2 β-lactamases.
b
The amino acid sequence of CDD-1, which was used as the reference sequence for comparison, is indicated in bold. The amino acid numbering indicates the position of each amino acid in the mature enzyme.