There are two errors in S2 Table. Labeling of primer sequences for PCR amplification are incorrect. Primers given for IAbβ-chain are specific for β2m and vice versa. Furthermore, there is a mistake in the sequence for the nested forward primer specific for β2m.
S2 Table. Primers used to for mutation analysis at genomic target sites.
| IAb β-chain |
forward | 5'-TTTGCTTTCTGAAGGGGGCA-3' |
| reverse | 5'-AGAATGGAGTCTCACTCTCTCTT-3' | |
| β2m | nested forward | 5'-TCTGAGTGGGATATTGTCAGC-3' |
| nested reverse | 5'-TTGCGCTCAGGGAGTCTA-3' | |
| forward | 5'-AAGGGTTGAGTTCTGCCAGTT-3' | |
| reverse | 5'-TCTCTCGACTTCGGTTGGAT-3' |
Reference
- 1.Das K, Eisel D, Lenkl C, Goyal A, Diederichs S, Dickes E, et al. (2017) Generation of murine tumor cell lines deficient in MHC molecule surface expression using the CRISPR/Cas9 system. PLoS ONE 12(3): e0174077 10.1371/journal.pone.0174077 [DOI] [PMC free article] [PubMed] [Google Scholar]