Fig 3 is incorrect. The authors have provided a corrected version here. The publisher apologizes for the error.
Fig 3. Validation of antibodies against AyNCXA.
A) The anti-AyNCXA antibodies recognize a ~100 kDa and ~75 kDa protein in homogenized A. yongei tissue. Both bands are eliminated when the antibody is pre-absorbed with the epitope peptide overnight, and neither band is present when the membrane is incubated with the pre-immune serum. All sample wells contain the same amount of protein and all three Western Blot images were taken at the same exposure. B) Immunofluorescence microscopy of A. yongei tissue reveals AyNCXA is present in all four tissue layers, including the calicodermis. C) Pre-absorption of antibodies with antigen peptide eliminates signal at the same exposure, confirming antibody specificity.
Reference
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