Table 4. List of single nucleotide polymorphisms identified in the pooled dataset of Fusarium graminearum strains.
Positions are aligned positions between the PH-1 reference sequence and the pooled sequences (“short” and “long”). “Reference” refers to the nucleotide found in the given reference sequence used for mapping, while “Alternative” refers to the nucleotide suggested by the mapped reads. Position 90,636 shows unusual ratios: in both mappings the reference nucleotide (C or A) has a frequency of 70% and the alternative nucleotide has 30%. This is due to an adjacent indel that affects the mapping results.
| PH-1 | Pooled | |||
|---|---|---|---|---|
| Position | Reference | Alternative | Reference | Alternative |
| 2,337 | A (0.77) | G (0.23) | A (0.77) | G (0.23) |
| 6,288 | C (0.41) | A (0.59) | A (0.61) | C (0.39) |
| 6,355 | T (0.42) | C (0.58) | C (0.60) | T (0.40) |
| 13,540 | C (0.78) | A (0.22) | C (0.78) | A (0.22) |
| 37,126 | C (0.75) | T (0.25) | C (0.75) | T (0.25) |
| 37,773 | A (0.75) | G (0.25) | A (0.75) | G (0.25) |
| 44,773 | A (0.62) | G (0.38) | A (0.62) | G (0.38) |
| 64,776 | G (0.53) | A (0.47) | G (0.53) | A (0.47) |
| 70,827 | A (0.62) | G (0.38) | A (0.62) | G (0.38) |
| 89,194 | G (0.57) | A (0.43) | G (0.57) | A (0.43) |
| 90,636 | C (0.70) | A (0.30) | A (0.70) | C (0.30) |
| 95,918 | A (0.43) | C (0.57) | C (0.59) | A (0.41) |
| 99,784 | A (0.40) | G (0.60) | G (0.62) | A (0.38) |
| 100,362 | C (0.42) | A (0.58) | A (0.59) | C (0.41) |
| 100,538 | G (0.42) | A (0.58) | A (0.61) | G (0.39) |