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. 2018 Dec 18;145(24):dev165381. doi: 10.1242/dev.165381

Fig. 6.

Fig. 6.

Gli1 lineage cells replace Sox9 lineage cells during enthesis maturation. (A,B) Sox9-CreERT2;R26R-tdTomato and Gli1-CreERT2;R26R-tdTomato mice were labeled by tamoxifen administration at E12.5 and E13.5, respectively, and sacrificed at E15.5-P14. Transverse sections through the DT (A) and TM (B) show an increase in Sox9 lineage cells at E15.5-P0. Between P0 and P14, a continuous decrease in Sox9 lineage cells is seen. Concurrently, Gli1 lineage cell number gradually increased in both DT and TM entheses. By P8, both entheses were populated by Gli1 lineage cells. (C,D) The percentages of tdTomato+ cells in Sox9-CreERT2;R26R-tdTomato and Gli1-CreERT2;R26R-tdTomato limbs were significantly different in the DT (C, P=0.0010) and TM (D, P=0.0019). Additionally, there was a significant decrease in the percentage of tdTomato+ cells in Sox9-CreERT2;R26R-tdTomato limbs over time (P=0.020). Dashed line demarcates the enthesis. Scale bars: 50 µm.