Table 1.
Bacterial strains and plasmids
| Plasmid or Strain | Relevant genotype or features | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| HB101 | F− mcrB mrr hsdS20(rB− mB−) recA13 leuB6 ara-14 proA2 lacY1 galK2 xyl-5 mtl-1 rpsL20 | B. Dupuy |
| MC277 | HB101 pRK24 pMC211 | (42) |
| MC901 | HB101 pRK24 pMC632 | This study |
| MC1038 | HB101 pRK24 pMC687 | This study |
| C. difficile | ||
| 630 | Clinical isolate | (53) |
| 630Δerm | ErmS derivative of strain 630 | (54) |
| MC1040 | 630Δerm pMC687 | This study |
| MC1041 | 630Δerm Δskin (ΔvanZ1) | This study |
| MC282 | 630Δerm pMC211 | (42) |
| MC907 | 630Δerm pMC632 | This study |
| MC1260 | Δskin (ΔvanZ1) pMC211 | This study |
| MC1261 | Δskin (ΔvanZ1) pMC632 | This study |
| Plasmids | ||
| pRK24 | Tra+, Mob+; bla, tet | (55) |
| pMC123 | E. coli-C. difficile shuttle vector; bla, catP | (37) |
| pMC211 | pMC123 PcprA | (42) |
| pMC632 | pMC211 PcprA::vanZ1 | This study |
| pMC687 | pMC211 PcprA::CD1234 | This study |