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. 2018 Dec 28;293(52):20012. doi: 10.1074/jbc.W118.007016

Withdrawal: Hepatitis C virus NS5A and subgenomic replicon activate NF- κ B via tyrosine phosphorylation of I κ B α and its degradation by calpain protease.

Gulam Waris, Antonia Livolsi, Veronique Imbert, Jean-Francois Peyron, Aleem Siddiqui
PMCID: PMC6311525  PMID: 30593533

VOLUME 278 (2003) PAGES 40778–40787

This article has been withdrawn by the authors. Antonia Livolsi, Véronique Imbert, and Jean-François Peyron participated in the study by providing IκBα mutant plasmids. An investigation by the Journal determined the following. A band was pasted over in the NS5A immunoblot in Fig. 2A. The actin immunoblot from Fig. 2A was reused as actin in 2B, NS5A in 4A, NS5A in 4B, actin in 7D, and actin in 9A. Additionally, this actin immunoblot was reused in the following publications representing different experimental conditions: Waris et al. (2005) J. Virol. 79, 1569–1580, Waris and Siddiqui (2005) J. Virol. 79, 9725–9734, Waris et al. (2007) J. Virol. 81, 8122–8130, Nasimuzzaman et al. (2007) J. Virol. 81, 10249–10257, Burdette et al. (2010) J. Gen. Virol. 91, 681–690, Burdette et al. (2012) J. Gen. Virol. 93, 235–246, Presser et al. (2013) PLOS One 8, e56367, and McRae et al. (2016) J. Biol. Chem. 291, 3254–3267. The EMSA shown in Fig. 3D was reused in Waris et al. (2005) J. Virol. 79, 1569–1580 representing different experimental conditions. Lanes 3 and 5 of the IκBα immunoblot in Fig. 9A were duplicated. The authors who performed the experiments were unable to clarify the Journal's concerns with original data. Gulam Waris does not agree that the actin immunoblot was reused in other publications. Aleem Siddiqui disagrees with the determination of the reuse of actin immunoblots in publications other than the JBC paper being withdrawn. The authors sincerely apologize to the readers.


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