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. 2018 Oct 29;6(4):113. doi: 10.3390/microorganisms6040113

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Fluorescence microscopy observations of no flow biofilm matrix structure obtained from UAS391-Ery^S, argininosuccinate lyase (argH), fumarate hydratase class II (fumC), succinate dehydrogenase (flavoprotein subunit) (sdhA), succinate dehydrogenase iron-sulfur protein (sdhB), and citrate synthase II (gltA) Tn mutants. Since microscopy images of gltA::Tn, acnA::Tn, icd::Tn, sucC::Tn, and rocF::Tn, as well as corresponding complemented mutant biofilms were comparable to each other, the matrix formed by gltA::Tn serves as an example picture for all. The top row shows total cells stained with SYTO™ 9 and PI. The middle row shows PNAG stained with WGA Texas Red™-X Conjugate, and is combined with Bright-field imaging. The bottom row shows the protein component stained with FilmTracer™ SYPRO™ Ruby Biofilm Matrix.