Table 2.
Overview of results. Quantification of formed biofilm mass (optical density, no flow assay; integrated density, dynamic assay), growth rate (growth curve assay), ratio live:dead cells (Syto™ 9 Green Fluorescent Acid and propidium iodide stain), protein component (Filmtracer™ SYPRO™ Ruby Biofilm Matrix stain), and PIA/PNAG component (WGA Texas Red™-X Conjugate stain). Standard deviations are mentioned next to each value (±) and the percentage value compared to UAS391-EryS is mentioned between brackets. NT refers to not tested.
| Strain | Optical Density (OD492) | Integrated Density (Fluorescence in Pixels) | Growth Rate (min−1) | Ratio Live:Dead Cells (%) | Protein Component (%) | PIA/PNAG Component (%) |
|---|---|---|---|---|---|---|
| UAS391-Erys | 0.814 ± 0.14 (100%) | 11,301 ± 61 (100%) | 0.157 ± 0.01 | 61:39 ± 3:1 | 100 ± 3 | 100 ± 6 |
| ATCC® 25923™ | NT | NT | NT | 58:42 ± 13:4 | 34 ± 8 | 835 ± 2 |
| argH::Tn | 0.504 ± 0.07 (62%) | 3653 ± 45 (32%) | 0.161 ± 0.01 | 44:56 ± 2:1 | 48 ± 10 | 110 ± 15 |
| argH::Tn with pGV5990 | 0.828 ± 0.15 (102%) | 9389 ± 66 (83%) | NT | 59:41 ± 16:5 | 83 ± 2 | 106 ± 16 |
| acnA::Tn | 0.782 ± 0.11 (96%) | 651 ± 036 (58%) | 0.163 ± 0.01 | 61:39 ± 2:1 | 64 ± 3 | 77 ± 4 |
| acnA::Tn with pGV6007 | 0.805 ± 0.15 (99%) | 773 ± 57 (68%) | NT | 60:40 ± 10:3 | 92 ± 16 | 101 ± 11 |
| icd::Tn | 0.838 ± 0.13 (103%) | 8264 ± 16 (73%) | 0.166 ± 0.01 | 58:42 ± 13:4 | 67 ± 4 | 110 ± 17 |
| icd::Tn with pGV5994 | 1.143 ± 0.12 (141%) | 8308 ± 163 (74%) | NT | 57:43 ± 15:5 | 91 ± 3 | 100 ± 20 |
| icd::Tn with pGV5999 | 1.248 ± 0.17 (153%) | 8076 ± 133 (72%) | NT | 54:46 ± 19:7 | 96 ± 22 | 101 ± 9 |
| gltA::Tn | 0.789 ± 0.11 (97%) | 7911 ± 11 (70%) | 0.157 ± 0.01 | 57:43 ± 18:6 | 61 ± 10 | 91 ± 5 |
| gltA::Tn with pGV5992 | 1.102 ± 0.12 (135%) | 9616 ± 25 (85%) | NT | 61:39 ± 5:2 | 93 ± 0 | 100 ± 12 |
| gltA::Tn with pGV5999 | 1.055 ± 0.10 (130%) | 8149 ± 27 (72%) | NT | 59:41 ± 7:2 | 89 ± 9 | 100 ± 2 |
| fumC::Tn | 0.374 ± 0.07 (46%) | 2065 ± 42 (18%) | 0.162 ± 0.01 | 43:57 ± 1:0 | 49 ± 0 | 122 ± 7 |
| fumC::Tn with pGV6005 | 0.941 ± 0.14 (116%) | 7662 ± 188 (68%) | NT | 66:34 ± 10:3 | 80 ± 5 | 100 ± 13 |
| sucC::Tn | 0.847 ± 0.17 (104%) | 7506 ± 58 (67%) | 0.150 ± 0.01 | 47:53 ± 19:6 | 54 ± 3 | 103 ± 17 |
| sucC::Tn with pGV5998 | 0.804 ± 0.11 (99%) | 8715 ± 10 (77%) | NT | 65:35 ± 15:5 | 96 ± 7 | 101 ± 16 |
| sucC::Tn with pGV6000 | 1.041 ± 0.13 (128%) | 7599 ± 159 (67%) | NT | 68:32 ± 11:4 | 83 ± 2 | 101 ± 5 |
| sdhA::Tn | 0.537 ± 0.11 (66%) | 3835 ± 60 (34%) | 0.162 ± 0.01 | 47:53 ± 5:2 | 33 ± 3 | 89 ± 8 |
| sdhA::Tn with pGV6001 | 1.002 ± 0.10 (123%) | 9002 ± 11 (80%) | NT | 51:49 ± 6:2 | 78 ± 8 | 101 ± 6 |
| sdhA::Tn with pGV6002 | 1.071 ± 0.11 (132%) | 7252 ± 092 (64%) | NT | 53:47 ± 4:1 | 92 ± 10 | 100 ± 11 |
| sdhB::Tn | 0.667 ± 0.10 (82%) | 4875 ± 056 (43%) | 0.159 ± 0.01 | 48:52 ± 5:2 | 44 ± 18 | 84 ± 7 |
| sdhB::Tn with pGV5996 | 0.908 ± 0.12 (112%) | 11,057 ± 129 (98%) | NT | 66:34 ± 6:2 | 106 ± 21 | 100 ± 13 |
| sdhB::Tn with pGV6002 | 1.140 ± 0.09 (140%) | 8455 ± 65 (75%) | NT | 49:51 ± 5:2 | 92 ± 3 | 100 ± 17 |
| rocF::Tn | 0.765 ± 0.12 (94%) | 7586 ± 15 (67%) | 0.163 ± 0.01 | 49:51 ± 5:2 | 51 ± 11 | 89 ± 19 |
| rocF::Tn with pGV6003 | 0.878 ± 0.09 (108%) | 8342 ± 21 (74%) | NT | 65:35 ± 12:4 | 84 ± 3 | 101 ± 8 |