Table 2.

Metabolites showing significantly altered levels during cell senescence in the MDA-MB-231 cell line and their related metabolic pathways.

MDA-MB-231 Cells
Metabolite	3 Day versus 0 Day		5 Day versus 3 Day		Involved Pathway
	Fold Change	p-Value	Fold Change	p-Value
Malic acid	0.33	0.00045	0.91	0.61	TCA cycle
Fumaric acid	0.44	0.0019	0.88	0.48
Citric acid	0.2	0.019	2.68	0.1
Alanine	17.62	0.019	0.47	0.042	Amino acid metabolism
Leucine	2.21	0.00073	0.66	0.0038
Isoleucine	2.6	0.002	0.59	0.00035
Proline	3.42	0.01	0.43	0.01
Glycine	2.2	0.0029	0.47	0.02
Threonine	2.69	0.0053	0.47	0.0036
Glutamic acid	1.97	0.0056	0.43	0.00249
Phenylalanine	4.34	0.014	0.46	0.026
2-Aminoadipic acid	1.83	0.012	0.42	0.0045
Tyrosine	2.96	0.0028	0.85	0.021
L-Tryptophan	5.61	0.029	0.48	0.05
Heptadecanoic acid	20.42	0.00043	1.55	0.17	Fatty acid metabolism
Oleic acid	1.27	0.04	0.9	0.2
Stearic acid	0.58	0.0029	1.48	0.19
2-Keto-3-methylvaleric acid	2.98	0.03	1.12	0.57	BCAA metabolism
Isobutyric acid	4.86	0.023	1.13	0.43
Glycerol-3-phosphate	21.28	0.0065	1.046	0.81	Nucleic acid metabolism
Creatinine	10.81	0.026	0.75	0.23
5-Methylthioadenosine	3.7	0.0085	0.65	0.045
Phosphorylethanolamine	63.71	0.035	0.78	0.52	Others
Pantothenic acid	2.45	0.0075	0.94	0.73
Scyllo-inositol	0.69	0.023	1.54	0.26
D-Myo-inositol	1.57	0.0037	0.68	0.13
Cellobiose	3.7	0.0085	0.65	0.045
Cholest-8(14)-en-3-ol	19.94	0.00052	0.61	0.044