Skip to main content
. 2018 Dec 1;7(12):241. doi: 10.3390/cells7120241

Figure 1.

Figure 1

Schematic representation of virus constructs used in this study. From top to bottom: DENV2 full-length genome; DVR2A, derived from the DENV2 full-length genome by insertion of a Renilla luciferase (R-Luc) coding sequence downstream of the capsid cyclization sequence (CAE) and upstream of a Tosea asigna virus 2A protease cleavage site; sgDVR2A, a subgenomic reporter replicon derived from the DENV2 full-length genome by insertion of R-Luc coding sequence in-between CAE and the 2A cleavage site sequence. The last 24 amino acid residues of the envelope coding region (TM) at the N-terminus of NS1 ensure proper membrane topology of the polyprotein. sgDVR2A-GND, a replication-deficient NS5 mutant of sgDVR2A. All DENV constructs are derived from the DVs2 16681 isolate. JcR2a, a Jc1 (J6CF-JFH1 chimera) derivative containing the R-Luc gene fused N-terminally to 16 codons of the core gene (C) and C-terminally to the FMDV 2A protease cleavage site (grey striped box); Black bars in all panels indicate UTRs. Polyprotein cleavage products are labeled as specified in the introduction.