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. 2018 Dec 11;9(97):37054–37068. doi: 10.18632/oncotarget.26457

Figure 6. EZH2 inhibition decreases the levels of H2A. Z.

Figure 6

(A) Western blot analyses of control, vehicle and GSK126 treated LNCaP cells immunoblotted using EZH2, H3K27me3 and H2A.Z antibodies. (A-1) Representative image. (A-2) Graphical representation of the western blot data shown in (A-1). The data is a representation of three independent experiments normalized using H4. (B) LNCaP cells were exposed to GSK126 and subsequently treated with metformin. Protein lysates obtained from control, metformin and a combination of GSK126 and metformin treated cells were analysed by western blot using H3K27me3 and H2A.Z antibodies. (B-1) Representative western blot image. (B-2) Graphical representation of the western blot data from 3 independent experiments. Student’s t- tests were used to calculate significance; P value, * P < 0.05, **P < 0.01, ***P < 0.001. H4 was used as a normalizer. Control: Starting LNCaP/C4-2 cells; Vehicle: DMSO; GSK 126: GSK 126 treated cells.