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. Author manuscript; available in PMC: 2019 Jan 6.
Published in final edited form as: Leukemia. 2018 Jul 5;33(1):75–87. doi: 10.1038/s41375-018-0188-8

Figure 5. Activation of Btk in the primary bone marrow cells of Q741x mutant mice.

Figure 5

(a) Representative immunoblots using lysates of c-Kit+ bone marrow cells after 2 hour serum starvation and G-CSF stimulation (TAS: Time after Stimulation). The experiment was performed twice (n=2). (b) Ibrutinib dose curves of c-Kit+ bone marrow cell viability. IC50 values were calculated in Prism statistical analysis software using the three-parameter logistic method. The experiment was performed in three independent biological replicates (n=3). (c) Graphical representation of the colony forming units (CFU) of murine c-Kit+ bone marrow cells. The results represent three independent biological replicates (n=3) (**** = p value < 0.0001, student t-test).