Figure 6.

Effect of treatment on proliferation of renal tumor cells in Tsc2^+/− mice.

Kidney sections were prepared from 14-month-old Tsc2^+/− mice treated as described above and stained with antibody against Ki67 by IHC to assess proliferation of tumor cells. Fifteen or more renal tumors from each treatment group were randomly selected for proliferation analysis. Ki67-positive cells were identified using ImageJ, and percentage of Ki67-positive cells in total tumor cells was used for comparison. Representative sections were presented to show expression of Ki67 in tumor cells. Black lines are scale bars. The right bottom panel shows comparison of percentage of Ki67-positive tumor cells between vehicle and other treatment groups (see Supplementary Tale 3 for detailed statistical analysis.).