Table 1.

Summary of Animal Treatment

Treatment Group	Number of Tsc2+/− Mice	Number of Males	Number of Females	Treatment Start Age (Months)	Treatment End Age (Months)	Dosage⁎	Number of Animals Killed Due to Loss of Body Weight Within the First Month of Treatment†
Vehicle	17	8	9	12	14	10 μl/g	0
3-BrPA	19	9	10	12	14	3 mg/kg	3
CB-839	20	9	11	12	14	200 mg/kg	0
Rapamycin	20	8	12	12	14	4 mg/kg	0
3-BrPA+rapamycin	18	8	10	12	14	3 mg/kg+ 4 mg/kg	3
CB-839+rapamycin	20	8	12	12	14	200 mg/kg+ 4 mg/kg	0
3-BrPA+CB-839	18	8	10	12	14	3 mg/kg+ 200 mg/kg	4

^⁎Tsc2^+/− mice were treated twice daily with vehicle or CB-839 via gavage and five times a week with 3-BrPA or rapamycin via intraperitoneal injection.

^†These mice were killed due to significant loss of body weight within the first month of treatment.