Table 4. HP1a-deficient embryos exhibit various mitotic defects.
| Tubulin | Cnn | PH3 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Maternal genotype | Diffuse spindles | Multiple spindle types (asynch. div.) | Normal expression without HP1a | Absent in the present of HP1a | n | Absent | Double | Multiple | n | Varied expression (asynch. div.) | n |
| w1118 | 20 (0.0%) | 5 (0.0%) | 0 | 4 (0.0%) | 707 | 0 | 6 (0.0%) | 0 | 488 | 15 (0.0%) | 369 |
| Su(var)2-504 | 16 (0.0%) | 2 (0.0%) | 0 | 3 (0.0%) | 554 | 1 (0.0%) | 0 | 2 (0.0%) | 476 | 6 (0.0%) | 552 |
| Su(var)2-504; HP1a RNAi#1 | 400 (61.0%) | 460 (70.1%) | 320 (48.8%) | 402 (61.2%) | 656 | 460 (76.8%) | 481 (80.1%) | 403 (67.2%) | 599 | 432 (68.7%) | 629 |
| Su(var)2-504; EGFP RNAi#1 | 14 (0.0%) | 0 | 0 | 2 (0.0%) | 465 | 1 (0.0%) | 7 (0.0%) | 0 | 552 | 7 (0.0%) | 443 |
| Su(var)2-504; HP1a RNAi#2 | 445 (74.4%) | 485 (81.1%) | 189 (31.6) | 423 (70.7%) | 598 | 503 (78.3%) | 559 (87.0%) | 398 (62.0%) | 642 | 511 (78.6%) | 650 |
| Su(var)2-504; EGFP RNAi#2 | 16 (0.0%) | 3 (0.0%) | 0 | 0 | 556 | 0 | 8 (0.0%) | 7 (0.0%) | 494 | 14 (0.0%) | 552 |
For each maternal genotype, the percentage of embryos with the indicated phenotypes was calculated from the total number (n) counted in two independent experiments. “Asynch. div.” is short for “asynchronous division.” “Multiple spindle types” for tubulin indicates spindles found in multiple stages of mitosis within the same embryo. “Absent” phenotype in Cnn indicates any nucleus with no surrounding Cnn expression. “Varied expression” for PH3 indicates that not all nuclei exhibited uniform PH3 expression (all or none) and thus signifies asynchronous nuclear division. Percentage reported in the Results is the average percentage from those of HP1a RNAi #1 and HP1a RNAi #2. asynch. div., asynchronous division; RNAi, RNA interference; EGFP, enhanced GFP.