Figure 2.

The Pex5^MI06050 mutation affects CNS, PNS, and muscle structure. (A) The repeated segmental patterns of neurons of the CNS and PNS marked by anti-Futsch, CNS axons marked by anti-Even-skipped, and glial cells (except midline glia) marked by anti-Repo in control embryos (stage 15) were disrupted in Pex5^MI06050/Pex5^MI06050 embryos. Bar, 10 μm. (B) The repeated segmental pattern of developing muscles marked by anti-myosin II (MyoII) in control embryos was disrupted in Pex5^MI06050/Pex5^MI06050 embryos (stage 15). Bar, 10 μm. (C) Mature peroxisomes (punctate anti-SKL signal, red) are observed in the larval midgut. In Pex5^MI06050/Pex5^MI060 mutants, diffuse cytosolic anti-SKL staining and anti-SKL–positive aggregates indicate PTS1 import was impaired. DAPI-labeled nuclei are in blue. Bar, 2 μm. (D) Pex5^MI06050/Pex5^MI06050 mutants have lower amounts of C₁₄, C₁₆, C₁₈, and C₂₀ fatty acids and greater amounts of C₂₂ and C₂₄ fatty acids compared to control animals. Values are averages of four independent experiments ± SD; 1000 larvae per sample per each genotype were used in each replicate (4000 larvae total). Significance was determined using Student’s t-test; *** P < 0.001; ** P < 0.01. (E) Pex5^MI06050/Pex5^MI06050 embryos exhibit greater numbers of TUNEL-positive cells than control embryos. Images are representative of five independent experiments. N = 20 per experiment per genotype. Bar, 10 μm.