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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Neuropeptides. 2018 Nov 30;73:78–88. doi: 10.1016/j.npep.2018.11.009

Figure 2: NPY and Y1 are locally expressed in bone tissue.

Figure 2:

NPY (A) and Y1 expression (B) in hypothalamus and bone tissue. NPY (C) and Y1 (D) expression in primary cell cultures of bone marrow monocytes/macrophages (BMMs) stimulated with mCSF only (day 1) and in response to mCSF and RANKL stimulation (M+R; days 1–4), bone marrow stromal cells (BMSCs) undergoing differentiation (days 7, 14, 21) and calvarial osteoblasts (cOB) (day 4, 7, 11, 14). Mean±SEM. All samples were normalized to hypothalamic levels. Expression was not detectable (ND) by qPCR. Statistical analyses: one-way ANOVA, where *p<0.05 and **p<0.01. BMM cultures – n = 3; BMSC culture – n = 2; calvarial osteoblasts – n =2.