Figure 3.

SRV2 Deletion Does Not Affect Dnm1 Distribution

(A) Dnm1-HA levels in mitochondrial and cytosolic fractions of wild-type (WT) and Δsrv2 cells were evaluated by immunoblotting. Pgk1 and porin were used as cytosolic (Cyto) and mitochondrial (Mito) markers, respectively. Mitochondrial Dnm1 levels were normalized to porin levels for comparisons. Mean ± SD from three trials are presented.

(B) Wild-type, Δsrv2, and Δfis1 cells expressing mt-DsRed and Dnm1-GFP were imaged by fluorescence microscopy. Dnm1-GFP foci on mt-DsRed were analyzed using ImageJ software. Mander's coefficient indicates the degree of association of Dnm1-GFP and mt-DsRed. ***p < 0.001 vs. WT group. Means ±SD are presented; 25 yeast cells/strain. Scale bars represent 5 μm.