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. 2019 Jan 10;14(1):e0210895. doi: 10.1371/journal.pone.0210895

Correction: Differential expression of pathogenic genes of Entamoeba histolytica vs E. dispar in a model of infection using human liver tissue explants

Cecilia Ximénez, Enrique González, Miriam Nieves, Ulises Magaña, Patricia Morán, Marco Gudiño-Zayas, Oswaldo Partida, Eric Hernández, Liliana Rojas-Velázquez, Ma Carmen García de León, Héctor Maldonado
PMCID: PMC6328173  PMID: 30629702

Table 4 is incorrect. Please see the correct Table 4 here.

Table 4. Expression levels of genes linked to mechanisms of pathogenesis in the ex vivo infection model, selected by bibliographic revision.

CLAVE Time
(h)
RQ ± SD p value
E. histolytica E. dispar
Ehoxired 1 6049 ± 1527 3822 ± 1231 0.072
3 2320 ± 127.3 820.5 ± 269 0.045
24 592.2 ± 125 75 ± 25 0.033
48 21 ± 12.5 0.8 ± 0.23 0.025
Ehaig-1 1 80.3 ± 32.3 16.3 ± 2.6 0.04
3 361.1 ± 72.4 0.64 ± 0.4 0.03
24 4154. 4 ± 201 0.21 ± 0.1 0.02
48 3.4 ± 1.2 0.96 ± 0.4 0.017
Eh hsp-20kDa 1 957.2 ± 191 27.4 ± 6.6 0.036
3 1236.8 ± 327 39.2 ± 6.1 0.023
24 29.3 ± 5.6 9.5 ± 6.5 0.03
48 35.8 ± 9.1 0.05 ± 0.0006 0.04
Ehpeptidase 1 2.1 ± 0.6 0.044 ± 0.014 0.015
3 8.25 ± 1.7 0.66 ± 0.25 0.026
24 2.7± 1.9 0.43 ± 0.12 0.045
48 2.23 ± 0.23 0.05 ± 0.03 0.04
Eh20kDa 1 0.37 ± 0.1 0.66 ± 0.4 0.93
3 70.7 ± 28 0.3 ± 0.2 0.042
24 4.1 ± 0.33 0.71 ± 0.4 0.015
48 2.3 ± 0.3 0.55 ± 0.4 0.14
Eh dovasa 1 0.41 ± 0.03 0.9 ± 05 0.18
3 2.1 ± 0.65 0.51 ± 0.2 0.032
24 0.9 ± 0.3 0.15 ± 0.01 0.046
48 0.9 ± 0.14 0.12 ± 0.043 0.042
Ehthiored 1 0.31 ± 0.023 0.31 ± 0.023 0.083
3 0.22 ± 0.1 0.13 ± 0.05 0.067
24 0.5 ± 0.03 0.2 ± 0.1 0.042
48 0.7 ± 0.14 0.2 ± 0.1 0.03
Ehhypo1 1 69 ± 32 0.23 ± 0.1 0.032
3 44.4 ± 1.3 0.9 ± 0.2 0.03
24 61.4 ± 25 0.53 ± 0.14 0.02
48 40 ± 15.4 0.5 ± 0.1 0.02
Ehhypo3 1 1.02 ± 0.4 1.7 ± 0.001 0.125
3 0.21 ± 0.1 13.2 ± 4.7 0.031
24 0.98 ± 0.7 7.4 ± 0.01 0.046
48 0.22 ± 0.01 0.76 ± 0.5 0.062
Ehhypo4 1 34.82± 0.4 5.4 ± 2.3 0.017
3 8.73 ± 2.5 6.2 ± 1 0.82
24 18.3 ± 6.1 17.1 ± 5 0.062
48 15.1 ± 6 0.53 ± 0.3 0.032
Ehhypo5 1 191.6 ±71.5 914.9 ± 95.5 0.15
3 1053 ± 33.6 3.6 ± 2.2 0.017
24 3.9 ±1.3 0.27 ± 0.1 0.033
48 0.75 ± 0.31 0 .63 ± 0.4 0.072
Ehhypo6 1 73.01 ± 32 0.01 ± 0.001 0.034
3 145.1 ± 63 0.034 ± 0.014 0.021
24 2.6 ± 0.7 0.02 ± 0.005 0.045
48 1.71 ± 0.7 0.01 ± 0.01 0.043
Ehhypo7 1 0.00011 ± 0.00006 0.0083 ± 0.0006 0.45
3 3.7 ± 0.85 0.48 ± 0.05 0.29
24 286.1 ± 57.1 0.87 ± 0.45 0.017
48 2.1 ± 0.45 0.15 ± 0.05 0.124
Ehypo8 1 6.4 ± 2.6 3.2 ± 0.5 0.083
3 13.6 ± 0.43 4.8 ± 1.5 0.02
24 2.83 ± 0.5 1.3 ± 0.23 0.31
48 0.95 ± 0.3 0.31 ± 0.02 0.073
Ehhypo9 1 3.1 ± 1.2 8.13 ± 0.95 0.02
3 0.56 ± 0.29 0.56 ± 0.4 0.03
24 129.4 ± 27.6 0.18 ± 0.1 0.025
48 2.2 ± 1.1 0.44 ± 0.04 0.04
Ehhypo10 1 0.74 ± 0.3 0.05 ± 0.013 0.097
3 0.012 ± 0.006 0.045 ± 0.004 0.94
24 0.041 ± 0.003 0.01 ± 0.004 0.31
48 0.05 ± 0.01 0.00023 ± 0.0001 0.88

After human PCLS were ex vivo infected with E. histolytica or E. dispar trophozoites for different time spans (1, 3, 24 and 48 h), the relative quantification (RQ) was determined (by qPCR) for the mRNA of some genes obtained by a bibliographic analysis. (RQ) relative quantification, (SD) standard deviation, data expressed as the mean of 5 separate assays. Shown the RQ of some genes which codified to hypothetical proteins, the cardinal number added is only for personal identification. p value calculated by Student t-test.

Reference

  • 1.Ximénez C, González E, Nieves M, Magaña U, Morán P, Gudiño-Zayas M, et al. (2017) Differential expression of pathogenic genes of Entamoeba histolytica vs E. dispar in a model of infection using human liver tissue explants. PLoS ONE 12(8): e0181962 10.1371/journal.pone.0181962 [DOI] [PMC free article] [PubMed] [Google Scholar]

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