Table 4 is incorrect. Please see the correct Table 4 here.
Table 4. Expression levels of genes linked to mechanisms of pathogenesis in the ex vivo infection model, selected by bibliographic revision.
| CLAVE | Time (h) |
RQ ± SD | p value | |
|---|---|---|---|---|
| E. histolytica | E. dispar | |||
| Ehoxired | 1 | 6049 ± 1527 | 3822 ± 1231 | 0.072 |
| 3 | 2320 ± 127.3 | 820.5 ± 269 | 0.045 | |
| 24 | 592.2 ± 125 | 75 ± 25 | 0.033 | |
| 48 | 21 ± 12.5 | 0.8 ± 0.23 | 0.025 | |
| Ehaig-1 | 1 | 80.3 ± 32.3 | 16.3 ± 2.6 | 0.04 |
| 3 | 361.1 ± 72.4 | 0.64 ± 0.4 | 0.03 | |
| 24 | 4154. 4 ± 201 | 0.21 ± 0.1 | 0.02 | |
| 48 | 3.4 ± 1.2 | 0.96 ± 0.4 | 0.017 | |
| Eh hsp-20kDa | 1 | 957.2 ± 191 | 27.4 ± 6.6 | 0.036 |
| 3 | 1236.8 ± 327 | 39.2 ± 6.1 | 0.023 | |
| 24 | 29.3 ± 5.6 | 9.5 ± 6.5 | 0.03 | |
| 48 | 35.8 ± 9.1 | 0.05 ± 0.0006 | 0.04 | |
| Ehpeptidase | 1 | 2.1 ± 0.6 | 0.044 ± 0.014 | 0.015 |
| 3 | 8.25 ± 1.7 | 0.66 ± 0.25 | 0.026 | |
| 24 | 2.7± 1.9 | 0.43 ± 0.12 | 0.045 | |
| 48 | 2.23 ± 0.23 | 0.05 ± 0.03 | 0.04 | |
| Eh20kDa | 1 | 0.37 ± 0.1 | 0.66 ± 0.4 | 0.93 |
| 3 | 70.7 ± 28 | 0.3 ± 0.2 | 0.042 | |
| 24 | 4.1 ± 0.33 | 0.71 ± 0.4 | 0.015 | |
| 48 | 2.3 ± 0.3 | 0.55 ± 0.4 | 0.14 | |
| Eh dovasa | 1 | 0.41 ± 0.03 | 0.9 ± 05 | 0.18 |
| 3 | 2.1 ± 0.65 | 0.51 ± 0.2 | 0.032 | |
| 24 | 0.9 ± 0.3 | 0.15 ± 0.01 | 0.046 | |
| 48 | 0.9 ± 0.14 | 0.12 ± 0.043 | 0.042 | |
| Ehthiored | 1 | 0.31 ± 0.023 | 0.31 ± 0.023 | 0.083 |
| 3 | 0.22 ± 0.1 | 0.13 ± 0.05 | 0.067 | |
| 24 | 0.5 ± 0.03 | 0.2 ± 0.1 | 0.042 | |
| 48 | 0.7 ± 0.14 | 0.2 ± 0.1 | 0.03 | |
| Ehhypo1 | 1 | 69 ± 32 | 0.23 ± 0.1 | 0.032 |
| 3 | 44.4 ± 1.3 | 0.9 ± 0.2 | 0.03 | |
| 24 | 61.4 ± 25 | 0.53 ± 0.14 | 0.02 | |
| 48 | 40 ± 15.4 | 0.5 ± 0.1 | 0.02 | |
| Ehhypo3 | 1 | 1.02 ± 0.4 | 1.7 ± 0.001 | 0.125 |
| 3 | 0.21 ± 0.1 | 13.2 ± 4.7 | 0.031 | |
| 24 | 0.98 ± 0.7 | 7.4 ± 0.01 | 0.046 | |
| 48 | 0.22 ± 0.01 | 0.76 ± 0.5 | 0.062 | |
| Ehhypo4 | 1 | 34.82± 0.4 | 5.4 ± 2.3 | 0.017 |
| 3 | 8.73 ± 2.5 | 6.2 ± 1 | 0.82 | |
| 24 | 18.3 ± 6.1 | 17.1 ± 5 | 0.062 | |
| 48 | 15.1 ± 6 | 0.53 ± 0.3 | 0.032 | |
| Ehhypo5 | 1 | 191.6 ±71.5 | 914.9 ± 95.5 | 0.15 |
| 3 | 1053 ± 33.6 | 3.6 ± 2.2 | 0.017 | |
| 24 | 3.9 ±1.3 | 0.27 ± 0.1 | 0.033 | |
| 48 | 0.75 ± 0.31 | 0 .63 ± 0.4 | 0.072 | |
| Ehhypo6 | 1 | 73.01 ± 32 | 0.01 ± 0.001 | 0.034 |
| 3 | 145.1 ± 63 | 0.034 ± 0.014 | 0.021 | |
| 24 | 2.6 ± 0.7 | 0.02 ± 0.005 | 0.045 | |
| 48 | 1.71 ± 0.7 | 0.01 ± 0.01 | 0.043 | |
| Ehhypo7 | 1 | 0.00011 ± 0.00006 | 0.0083 ± 0.0006 | 0.45 |
| 3 | 3.7 ± 0.85 | 0.48 ± 0.05 | 0.29 | |
| 24 | 286.1 ± 57.1 | 0.87 ± 0.45 | 0.017 | |
| 48 | 2.1 ± 0.45 | 0.15 ± 0.05 | 0.124 | |
| Ehypo8 | 1 | 6.4 ± 2.6 | 3.2 ± 0.5 | 0.083 |
| 3 | 13.6 ± 0.43 | 4.8 ± 1.5 | 0.02 | |
| 24 | 2.83 ± 0.5 | 1.3 ± 0.23 | 0.31 | |
| 48 | 0.95 ± 0.3 | 0.31 ± 0.02 | 0.073 | |
| Ehhypo9 | 1 | 3.1 ± 1.2 | 8.13 ± 0.95 | 0.02 |
| 3 | 0.56 ± 0.29 | 0.56 ± 0.4 | 0.03 | |
| 24 | 129.4 ± 27.6 | 0.18 ± 0.1 | 0.025 | |
| 48 | 2.2 ± 1.1 | 0.44 ± 0.04 | 0.04 | |
| Ehhypo10 | 1 | 0.74 ± 0.3 | 0.05 ± 0.013 | 0.097 |
| 3 | 0.012 ± 0.006 | 0.045 ± 0.004 | 0.94 | |
| 24 | 0.041 ± 0.003 | 0.01 ± 0.004 | 0.31 | |
| 48 | 0.05 ± 0.01 | 0.00023 ± 0.0001 | 0.88 | |
After human PCLS were ex vivo infected with E. histolytica or E. dispar trophozoites for different time spans (1, 3, 24 and 48 h), the relative quantification (RQ) was determined (by qPCR) for the mRNA of some genes obtained by a bibliographic analysis. (RQ) relative quantification, (SD) standard deviation, data expressed as the mean of 5 separate assays. Shown the RQ of some genes which codified to hypothetical proteins, the cardinal number added is only for personal identification. p value calculated by Student t-test.
Reference
- 1.Ximénez C, González E, Nieves M, Magaña U, Morán P, Gudiño-Zayas M, et al. (2017) Differential expression of pathogenic genes of Entamoeba histolytica vs E. dispar in a model of infection using human liver tissue explants. PLoS ONE 12(8): e0181962 10.1371/journal.pone.0181962 [DOI] [PMC free article] [PubMed] [Google Scholar]
