Collapsin Response Mediator Protein 1, a Novel Marker Protein for Differentiated Odontoblasts

Abstract

We previously reported that the terminal differentiation of odontoblasts was inhibited in Runx2 transgenic {Tg(Col1a1-Runx2)} mice under the control of the 2.3-kb Col1a1 promoter. Odontoblasts in Tg(Col1a1-Runx2) mice lose their characteristic long cellular processes, and show marked reductions in the protein levels of markers for odontoblasts, such as dentin sialophosphoprotein, nestin, and microtubule-associated protein tau (Mapt). We herein demonstrated that collapsin response mediator protein 1 (CRMP1), a neuronal phosphoprotein that participates in various aspects of neuronal development, was specifically expressed in the differentiated odontoblasts of wild-type, but not Tg(Col1a1-Runx2) tooth germs by comparing expression profiles in wild-type and Tg(Col1a1-Runx2) mouse molars using microarray and immunohistochemical analyses. CRMP1 expression was detected at a slightly later differentiation stage in odontoblasts than type 1 collagen, nestin, and Mapt expression, which was observed from the onset of dentinogenesis. Among these proteins, CRMP1 was the most specifically localized in odontoblasts in the tooth germ. In erupted molars, odontoblast-specific CRMP1 expression decreased with age. These results indicate that CRMP1 is a novel marker protein for differentiated odontoblasts in mouse tooth germs, and suggest that CRMP1 participates in the morphogenesis of functioning odontoblasts.

I. Introduction

Odontoblasts are specialized cells that are responsible for dentinogenesis, and differentiate from neural crest-derived cells of dental papillae [3, 13]. They are highly polarized cells that extend long processes into the dentinal tubules of dentine [3, 11, 22], and share structural and gene expression similarities with neurons [5, 6, 14]. However, the mechanisms underlying the formation of their unique cell morphology remain unknown. Although BMP/TGFβ and Wnt10a signaling pathways have been implicated in the terminal differentiation of odontoblasts from dental papilla cells [4], essential factors for the formation of odontoblastic processes have not yet been identified.

Runx2 is an essential transcription factor not only for the osteoblast differentiation and terminal differentiation of chondrocytes [12, 19, 30], but also for odontoblast differentiation [1, 8]. We previously analyzed Runx2 transgenic mice under the control of the 2.3-kb Col1a1 promoter {Tg(Col1a1-Runx2) mice} and demonstrated that Runx2 inhibited the terminal differentiation of odontoblasts [15]. In Tg(Col1a1-Runx2) mouse molars, odontoblasts lost their polarity and long cellular processes, and the expression levels of odontoblast marker proteins, including dentin sialophosphoprotein and nestin, were markedly reduced. Furthermore, a comparison of the gene expression profiles of molars in wild-type and Tg(Col1a1-Runx2) mice revealed that microtubule-associated protein tau (Mapt), which is a neuronal phosphoprotein that plays important roles in neuronal biology as well as microtubule dynamics and assembly, was strongly and specifically expressed in the odontoblasts of wild-type mouse molars [16]. Since the expression of Mapt was markedly reduced in Tg(Col1a1-Runx2) mouse molars, we suggested that Mapt participates in odontoblast morphogenesis, including the formation of cell processes, by regulating microtubule organization.

Collapsin response mediator protein 1 (CRMP1) is a member of the CRMP family, which is composed of five neuronal phosphoproteins (CRMP1-5) that are involved in neuronal development, maintenance, function, and disease [17, 21, 29]. In neuronal development, CRMP1 participates in neuronal cell migration, dendritic spine development, and synaptic plasticity [7, 25, 27, 28]. We herein demonstrate that CRMP1, the expression of which was also markedly reduced in Tg(Col1a1-Runx2) mouse molars, is a novel odontoblast-specific protein in mouse tooth germs.

II. Materials and Methods

Animals

Wild-type and Tg(Col1a1-Runx2) mice were maintained on a B6C3H F1 background. Prior to the present study, all experiments were reviewed and approved by the Animal Care and Use Committee of Nagasaki University Graduate School of Biomedical Sciences (No. 1403111129-20).

Gene expression microarray and real-time RT-PCR

Total RNA was extracted from the first and second molars of wild-type and Tg(Col1a1-Runx2) mice at 14 days of age using the acid guanidine thiocyanate-phenol-chloroform method according to the manufacturer’s instructions (Isogen, Nippon Gene, Tokyo, Japan). In the microarray analysis, poly(A) mRNA was purified from total RNA using the Oligotex kit (Takara, Tokyo, Japan). cRNA was amplified, labeled, and hybridized to Agilent SurePrint G3 Mouse Gene Expression Microarray 8 × 60K (Agilent Technologies, Santa Clara, CA). Hybridized microarray slides were scanned using an Agilent scanner. Relative hybridization intensities and background hybridization values were calculated using Agilent Feature Extraction Software (ver. 9.5.1.1). A real-time RT-PCR analysis was performed using the following primers as previously described [16]: CRMP1, 5'-CCCTGGAGGAGGGAATAA GAAG-3' and 5'-AGGTGCCGTGGAGATGTGT-3'; β-actin, 5'-CCACCCGCGAGCACAGCTTC-3' and 5'-TTGT CGACGACCAGCGCAGC-3'. We normalized values to that of β-actin.

Immunohistochemistry

Immunohistochemical analyses were conducted using 3-, 7-, 14-, and 21-day-old, 6- and 10-week-old, and 6-month-old mice. After anesthetization by an intraperitoneal injection of sodium pentobarbital, mice were perfused with 4% paraformaldehyde in 0.05% cacodylate buffer (pH 7.4), and dissected molars were further immersed in the same fixative at 4°C for 24 hrs. Tissues were then decalcified in 5% EDTA (pH 7.4) at 4°C for 1 day to 4 weeks and embedded in paraffin. Sections (thickness of 4 μm) were immunostained as described below for the detection of CRMP1, nestin, pro-collagen type 1a1 (Col1a1), and Mapt. A polyclonal rabbit anti-CRMP antibody (1:500 dilution; Abcam, Cambridge, UK), monoclonal mouse anti-nestin antibody (1:500 dilution; Chemicon International Inc., CA, USA), polyclonal rabbit anti-Col1a1 antibody (1:250 dilution; EMD Millipore Corp., CA, USA), and polyclonal rabbit anti-Mapt antibody (1:400 dilution; Dako, Glostrup, Denmark) were used as primary antibodies. Normal rabbit IgG (Dako, Glostrup, Denmark) for CRMP1, Col1a1, and Mapt, and normal mouse IgG₁ (Dako, Glostrup, Denmark) for nestin were used as negative controls instead of the primary antibodies. After blocking endogenous peroxidase activity with 0.3% H₂O₂ in methanol, sections were processed with Histofine Simple Stain MAX-PO (R) (Nichirei) for CRMP1, Col1a1, and Mapt antibodies, and the Histofine Mouse Stain Kit (Nichirei, Tokyo, Japan) for nestin. Antibody-binding sites were colored brown by an incubation with Histofine Simple Stain DAB solution (Nichirei). Sections were then counterstained with methyl green.

III. Results and Discussion

We previously investigated differentially expressed genes between Tg(Col1a1-Runx2) and wild-type mouse molars by a microarray analysis, and the genes belonging to four Gene Ontology (GO) terms including the word “cytoskeleton” were significantly enriched, while 38 genes with these GO terms were down-regulated (Z-score < −2.0 and ratio < 0.66) in Tg(Col1a1-Runx2) mouse molars [16]. In the present study, we selected 8 out of the 38 genes, for which signal intensities were greater than 100 in wild-type molars and expression in Tg(Col1a1-Runx2) mouse molars was markedly reduced (Z-score < −3.0 and ratio < 0.2-fold) (Table 1). Since odontoblasts share similar structures and gene expression patterns with neurons [5, 6, 14], we focused on genes that are predominantly expressed in the nervous system. CRMP1 (signal intensity in wild-type = 464.53, Z-score = −3.72, ratio = 0.18) as well as Mapt and Nestin, which were previously reported [15, 16], matched our focus. The validity of microarray data for CRMP1 expression was confirmed by real-time RT-PCR (Fig. 1). The expression level of CRMP1 in Tg(Col1a1-Runx2) molars was 0.05-fold that in wild-type molars, which consistent with and confirmed the accuracy of data obtained in the microarray analysis.

Table 1. .

List of down-regulated genes in Tg(Col1a1-Runx2) molars, which were included in significant Gene Ontology terms with the word “cytoskeleton” and showing signal intensity over 100 in wild-type molars and markedly reduced fold changes (Z-score < −3.0 and ratio < 0.2-fold) in Tg(Col1a1-Runx2) molars

Accessions	Genes	Signal intensity of wild-type	Z-score	ratio
NM_011474	small proline-rich protein 2H (Sprr2h)	397.08	−4.90	0.10
NM_001136058	collapsin response mediator protein 1 (Crmp1)	464.52	−3.72	0.18
NM_182992	myopalladin (Mypn)	575.88	−4.50	0.12
NM_198649	actin binding LIM protein family, member 3 (Ablim3)	6754.71	−4.95	0.16
NM_009922	calponin 1 (Cnn1)	8243.43	−5.96	0.11
NM_009132	scinderin (Scin)	8560.91	−4.95	0.16
NM_001038609	microtubule-associated protein tau (Mapt)	16443.69	−5.35	0.14
NM_016701	nestin (Nes)	20693.09	−6.37	0.14

* Z-scores and ratios (non-log-scaled fold change) were calculated from the normalized signal intensities of each probe for comparison between wild-type and Tg(Col1a1-Runx2) molars.

Fig. 1.

Real-time RT-PCR analysis of CRMP1 in tooth germs of 2-week-old mice. The value in wild-type (wt) mice was set as one and the relative level of Tg(Co1a1-Runx2) (tg) mice is shown. Data are the mean ± SD of seven wild-type and six tg mice. *P < 0.01.

We examined the localization of CRMP1 in mouse molars by immunohistochemistry, and found that CRMP1 was exclusively localized in the cytoplasm of differentiated odontoblasts and their processes in the tooth germs (Fig. 2a, b). CRMP1 expression was also detected in nerve fibers (Fig. 3a). In Tg(Col1a1-Runx2) mouse molars, in which odontoblasts lost their characteristic polarized morphology, CRMP1 expression in odontoblasts was not detectable (Fig. 2c, d). The expression of the odontoblast marker proteins, type 1 collagen (Col1a1), nestin, and Mapt was detected in differentiated odontoblasts with the onset of dentinogenesis in wild-type tooth germs, while CRMP1 expression was detected in odontoblasts at a slightly later differentiation stage than those for Col1a1, nestin, and Mapt (Fig. 3). In addition to odontoblasts, Col1a1 expression was detected in osteoblasts, fibroblasts, and collagen fibers, while nestin and Mapt expression was detected in dental pulp cells under the odontoblast layer (Fig. 3b–d). Therefore, among these proteins, CRMP1 was the most specifically localized in odontoblasts in the tooth germ (Fig. 3). These results support the applicability of CRMP1 as a useful marker protein for evaluating differentiated odontoblasts in mouse tooth germs even though its expression level was likely to be markedly lower than the other odontoblast marker proteins based on the signal intensities of microarray data (Table 1).

Fig. 2.

Expression of the CRMP1 protein in tooth germs of first molars in wild-type (wt) (a, b) and Tg(Col1a1-Runx2) (tg) (c, d) mice at 7 days of age. Boxed regions in a and c are magnified in b and d, respectively. CRMP1 expression was exclusively detected in differentiated odontoblasts in wild-type mice, whereas it was negligibly expressed in the odontoblasts of Tg(Col1a1-Runx2) mice. Bars = 200 μm (a and c), 40 μm (b and d).

Fig. 3.

Comparison of the expression of CRMP1 (a), Col1a1 (b), nestin (c), and Mapt (d) in tooth germs of first molars in 7-day-old wild-type mice. The expression of Col1a1, nestin, and Mapt in odontoblasts appeared with the onset of dentinogenesis (thick arrows), while CRMP1 expression in odontoblasts appeared after the onset of dentinogenesis (arrow head). Nerve fibers were also positive for CRMP1 and Mapt (thin arrows). The expression of Col1a1 was also observed in osteoblasts and collagen fibers, while that of nestin and Mapt was detected in dental pulp cells under the odontoblast layer (asterisks). Bars = 40 μm.

Microtubules are essential neuronal morphogenesis regulators, and CRMPs as well as MAPs are microtubule-regulating proteins. MAPs and CRMPs are categorized as microtubule-stabilizing proteins and microtubule-polymerizing proteins, respectively [20]. Previous studies reported that MAP2 and Mapt stabilized microtubules by binding along protofilaments [2], and CRMP2 bound to tubulin heterodimers to promote microtubule polymerization [9]. Since microtubules are essential constituents of odontoblast processes in addition to neurites [10, 18, 23, 24], CRMP1 may also be involved in odontoblast morphogenesis by regulating microtubule organization in cooperation with Mapt.

In erupted molars, the odontoblast-specific expression of CRMP1 was observed in most odontoblast layers at 3 weeks of age, decreased in coronal regions at 6 and 10 weeks of ages, and was not detectable in the whole region at 6 months of age (Fig. 4). The elongated odontoblasts at 3 weeks of age were taller than those at 6 months of age (Fig. 4), suggesting an association of CRMP1 expression and the morphology and function of odontoblasts. CRMP1 is strongly expressed in neurons after migration during brain development, but is down-regulated in the adult nervous system, except in the areas associated with brain plasticity or neurogenesis, such as the olfactory bulbs, hippocampus, and cerebellum [7, 26]. Since CRMP1 expression in odontoblasts also decreased with age, it may play an essential role in the morphogenesis of functioning odontoblasts during dentinogenesis.

Fig. 4.

Expression of the CRMP1 protein in erupted first molars in wild-type mice at 3 (a), 6 (c), and 10 weeks (e) and 6 months (g) of age. Cervical regions indicated by boxes in a, c, e and g are magnified in b, d, f and h, respectively. CRMP1 expression was down-regulated with age, starting from the coronal region, and was not detected in the whole region at 6 months of age. Bars = 200 μm (a, c, e and g), 20 μm (b, d, f and h).

In conclusion, CRMP1 is considered to be a novel marker protein for differentiated odontoblasts in mouse tooth germs, and may be an important microtubule-regulating protein for odontoblast morphogenesis in mice. Detailed studies on CRMP1 functions in dentinogenesis will be necessary in the future.

IV. Conflicts of Interest

The authors declare that there are no conflicts of interest associated with this manuscript.

V. Acknowledgments

This work was supported by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (#15K11015).