Analysis of NTRK Alterations in Pan-Cancer Adult and Pediatric Malignancies: Implications for NTRK-Targeted Therapeutics

Abstract

Purpose

Fusions that involve neurotrophic-tropomyosin receptor kinase (NTRK) genes are known drivers of oncogenesis. Therapies that target these ultra-rare, constitutionally active NTRK fusions have been remarkably effective. Herein, we analyze the prevalence of the full array of NTRK alterations—fusions, mutations, copy number alterations, and increased transcript expression—in diverse adult and pediatric tumor types to understand the landscape of NTRK aberrations in cancer.

Methods

We assessed 13,467 samples available from The Cancer Genome Atlas (adult tumors) and the St Jude PeCan database (pediatric tumors) for the prevalence of NTRK fusions, as well as associated genomic and transcriptomic co-aberrations in different tumor types.

Results

NTRK fusions were observed in 0.31% of adult tumors and in 0.34% of pediatric tumors. The most common gene partners were NTRK3 (0.16% of adult tumors) followed by NTRK1 (0.14% of pediatric tumors). NTRK fusions were found more commonly in pediatric melanoma (11.1% of samples), pediatric glioma (3.97%), and adult thyroid cancers (2.34%). Additional genomic and transcriptomic NTRK alterations—mutation, amplification, and mRNA overexpression—occurred in 14.2% of samples, whereas the frequency of alterations that implicated NTRK ligands and the NTRK co-receptor (p75^NTR) ranged from 3.8% to 5.4%. Among 31 adult samples carrying NTRK fusions, co-alterations occurred often and usually involved the downstream phosphoinositide-3-kinase signaling pathway, cell-cycle machinery, other tyrosine-kinase receptors, and mitogen-activated protein kinase signals.

Conclusion

Whereas NTRK fusions are exceedingly rare, other NTRK abnormalities affect 14% of patients with cancer. Affecting these alterations has not yet been achievable in cancer. Genomic co-alterations occur frequently with NTRK fusions, but it is not known if co-targeting them can attenuate primary or secondary resistance to NTRK inhibitors.

INTRODUCTION

NTRK1, NTRK2, and NTRK3 genes encode the neurotrophic-tropomyosin receptor tyrosine kinases (NTRKs) TrkA (NTRK1), TrkB (NTRK2), and TrkC (NTRK3). Ligands for the NTRK receptors are called neurotrophins. Nerve growth factor (NGF) binds to NTRK1; brain-derived neurotrophic factor (BDNF) and neurotrophin-4 (NT-4) and NT-5 bind to NTRK2; and NT-3 binds both NTRK1 and NTRK3.¹ Binding of neurotrophic factors to their receptors activates the downstream effectors of NTRK: phospholipase C-γ, mitogen-activated protein kinase (MAPK), and phosphatidylinositol-3-kinase (PI3K)/AKT pathways. In addition, neurotrophins also bind to the low-affinity NGF receptor p75^NTR. p75^NTR is a positive regulator of the NGF/NTRK1 system that reduces ligand-induced receptor ubiquitination and delays receptor internalization and degradation.²

NTRK receptors promote the proliferation and survival of neuronal cells^3-8 (Fig 1). Of interest, NTRK alterations induce tumorigenesis in both neurogenic and non-neurogenic cancers and are targets for therapeutic agents.^9-11 Although the clinical implications of NTRK single-nucleotide variants or copy number alterations are unclear, several NTRK transcript fusions have been identified. These drive NTRK mRNA and protein overexpression, which further leads to constitutive activation of downstream signaling.¹² The prevalence of NTRK fusions is low, but can reach more than 80% in some rare tumors, such as mammary-analog secretory carcinoma of the salivary gland, secretory breast carcinoma, and infantile congenital fibrosarcoma.^12-20 NTRK fusions are also found in 40% of pediatric non-brainstem high-grade glioma.²¹

Fig 1.

Neurotrophic-tropomyosin receptor tyrosine kinase (NTRK) receptor signaling pathway and inhibitors. The ligands nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin 3 (NT-3), and NT-4 bind to their receptors, namely NTRK1 (tropomyosin receptor kinase A or TrkA), NTRK2 (tropomyosin receptor kinase B or TrkB), and NTRK3 (tropomyosin receptor kinase C or TrkC). These receptors are under the regulation of the co-receptor p75 neurotrophin receptor (p75^NTR). The binding of the ligand to the receptor promotes to the dimerization of the receptor and its subsequent intracellular phosphorylation. Several signaling cascades are further activated—phospholipase Cγ (PLC-γ), mitogen-activated protein kinase (MAPK), and phosphoinositide-3-kinase (PI3K) —and are converging to protumorigenic cell processes, such as proliferation, survival invasion, or differentiation. The hyperactivation of the NTRK signaling pathway induced by NTRK alterations—fusions or point mutations—can be overcome by the use of NTRK antagonists (eg, ANA-12 and cyclotraxin B) or small-molecule tyrosine kinase inhibitors (eg, larotrectinib and entrectinib). For now, only small-molecule tyrosine kinase inhibitors are used in the clinic.

Among all alterations in NTRK genes, transcript fusions are currently the best characterized and the most pharmacologically tractable. Nonfusion NTRK alterations—for example, mutation or amplification—have been associated with a lack of response with some NTRK inhibitors.²² Because NTRK fusions are rare, the number of patients who can benefit from drugs that target NTRK receptors is relatively low, but the antitumor activity of such agents is remarkable.^23,24 Indeed, larotrectinib, a pan-NTRK inhibitor, demonstrated a response rate of 76% in patients with NTRK fusion–positive tumors (17 cancer types).^15,18 Tumor regression has been maintained for more than 1 year in 71% of patients. Entrectinib, an oral pan-NTRK, ROS1, and ALK inhibitor demonstrated a 79% objective response in patients with NTRK, ROS1, or ALK fusions.²²

In May 2017, a new precedent was set when an immune checkpoint inhibitor—pembrolizumab—was approved by the US Food and Drug Administration (FDA) for use in a tissue-agnostic fashion on the basis of a genomic biomarker (mismatch repair gene deficiency).²⁵ NTRK-selective inhibitors represent another pharmacology class that has been developed on the sole basis of somatic molecular patterns. Therefore, a comprehensive understanding of individual genomic alterations is becoming crucial.

In the current study, we assessed the landscape of NTRK genomic and transcriptomic alterations, as well as co-alterations in common signaling pathways, using a large cohort of samples available from The Cancer Genome Atlas (TCGA; adult, 33 tumor types) and the St Jude PeCan (pediatric, 17 tumor types).

METHODS

NTRK Receptor Fusions

Adult tumor NTRK-related transcript fusions were retrieved from The Jackson Laboratory Tumor Fusion Gene Data Portal.²⁶ These fusions were defined after an integrated analysis of paired-end RNA sequencing and DNA copy number data from TCGA that corresponded to 9,966 adult tumors (33 different tumor types).

Pediatric tumor NTRK-related transcript fusions were retrieved from the St Jude PeCan Data Portal database.²⁷ These fusions were defined after analysis of RNA sequencing data by the CICERO algorithm (Pediatric Cancer Genome Project) and corresponded to 3,501 pediatric tumors (17 different tumor types).^28,29

Genomic and Transcriptomic Alterations in NTRK Receptors, Co-Receptor, and Ligands (beyond fusions)

Adult and pediatric tumor NTRK-related mutations, copy number variations, and mRNA expression for NTRK receptors (NTRK1, NTRK2, and NTRK3), co-receptor (p75^NTR), and ligands (NGF, BDNF, NT-3, and NT-4) were retrieved from the UCSC Xena Portal.³⁰ These data include information on 13,467 samples from TCGA (n = 9,966 adults) and St Jude PeCan (n = 3,501 children) pan-cancer cohorts, of which 11,621 (n = 9,966 TCGA and n = 1,655 PeCan) had comprehensive information on fusions, mutations, and copy number alterations. Data were available without restriction of use on the date of February 1, 2018. All data used in this study respected the TCGA’s Human Subjects Protection and Data Access Policies³¹ and the St Jude Cloud Terms of Use.³²

Lists of significant variants were generated using whole-genome somatic mutation data and the MutSig2CV algorithm (http://www.broadinstitute.org/cancer/cga/mutsig), taking into account the somatic background mutation rate for each gene and its neighbor genes.³³

Focal copy number variations that correspond to genome-wide single-nucleotide polymorphism array data were normalized and assessed at the gene level using the GISTIC2 protocol,³⁴ where a deep loss was documented by the value (−2), a single-copy loss by the value (−1), a low-level gain by the value (+1), and an amplification by the value (+2). Only NTRK-related gene amplifications were kept for the analysis.

Sequencing-based mRNA expression signals were integrated and normalized for each gene per sample using the RNA-Sequencing by Expectation Maximization protocol. The standard score (z-score) for each gene per sample was calculated using the mean values and standard deviation found in all similar tumors—same tumor type—that are diploid for the said gene. A z-score of ≥ 1.96 standard deviation was used as the threshold of overexpression, whereas a threshold of ≤ −1.96 standard deviation was used to qualify underexpressed genes. Only NTRK-related mRNA overexpression was considered for the analysis.

Genomic and Transcriptomic Co-Alterations Occurring in NTRK Fusion–Positive Adult Tumors (n = 31 patients)

Comprehensive co-alteration data were not available in pediatric tumors. In adults, co-alterations within signaling cascades, such as TP53, MAPK, PI3K, tyrosine kinase receptor, or cell-cycle signaling pathways, were curated from TCGA. All nonsynonymous missense, nonsense, nonstop, deletion/insertions, frameshift, or splicing site mutations within the genes of interest, as well as deep losses or amplifications and mRNA under- or overexpressions, were kept for analysis.

RESULTS

Prevalence of NTRK Fusions in TCGA (adult) and St Jude PeCan (pediatric) Databases

NTRK Fusion Frequency in Adults

Of the 9,966 adult tumor samples in the TCGA database, 0.31% (n = 31 samples) presented an NTRK fusion. This alteration was most common in thyroid cancer (2.34% of samples), colon adenocarcinoma (0.97%), and low-grade glioma (0.94%). Twenty-two adult tumor types had no NTRK fusions. (There were 5,023 patient samples with these 22 NTRK fusion–negative tumor types [samples per tumor type = 36 to 541].) NTRK3 fusions were the most common (n = 16), followed by NTRK1 (n = 9) and NTRK2 (n = 6) fusions in adults (Table 1).

Table 1.

Frequency of NTRK Receptor Transcript Fusions in TCGA (n = 9,966 adult tumor samples) and St Jude Pediatric Cancer Database (n = 3,501 pediatric tumor samples), and Specific Tumors With High Incidence of NTRK Fusions in the Literature

NTRK Fusion Frequency in Children

Of the 3,501 pediatric tumor samples (St Jude PeCan database), 0.34% (n = 12) presented an NTRK fusion. Of interest, NTRK fusions were found in one of nine melanomas. NTRK fusions were also found in glioma (high and low grade [3.97%]) and B-cell acute lymphoblastic leukemia (0.14%). Thirteen pediatric tumor types (n = 2,524 patient samples) had no NTRK fusions (samples per tumor type = 26 to 714). Of 12 pediatric tumor samples with NTRK fusions, the most common partner gene was NTRK1 (n = 5) followed by NTRK2 (n = 4) and NTRK3 (n = 3; Table 1).

Therapeutic or Experimental Molecules With Activity Against NTRK Receptors

Overall, 32 molecules have demonstrated preclinical inhibition activity against one or more NTRK receptors^{35xref ref-type="bibr" rid="B37"/>-70} (Table 2). Surprisingly, five of these small inhibitors are already approved by the FDA for other indications, namely cabozantinib (Cabometyx; Exelixis, South San Francisco, CA; IC₅₀ against NTRK2, 7 nM), crizotinib (Xalkori; Pfizer, New York, NY; IC₅₀ against NTRK1 and NTRK2, 1 nM), midostaurin (Rydapt; Novartis, Basel Switzerland; IC₅₀ ranging from 11 to 51 nM), nintedanib (Ofev; Boehringer Ingelheim, Ingelheim am Rhein, Germany; IC₅₀ ranging from 17 to 264 nM), and regorafenib (Stivarga; Bayer, Leverkusen, Germany; IC₅₀ against NTRK1, 74 nM). It is not known if these five molecules exhibit clinical activity in patients who harbor NTRK-aberrant tumors. Sixteen molecules are currently being evaluated in clinical trials, with the most advanced being larotrectinib (Loxo Oncology, Stamford, CT; IC₅₀ for NTRK1, NTRK2, and NTRK3 fusions ranging from 4 to 9 nM). The new drug application was submitted to the FDA in December 2017 and granted priority review status on the basis of remarkable clinical activity²³ (Table 2).

Table 2.

Target Specificity and IC₅₀ of NTRK-Targeting Inhibitors

Types of NTRK-Related Alterations in Adult and Pediatric Tumors and Sensitivity to NTRK Inhibitors

To understand the potential benefits of selective NTRK inhibitors for the treatment of adult and pediatric patients with cancer, we first aimed to describe the prevalence and type of NTRK-activating pathway alterations, including point mutations, gene copy number amplifications, and mRNA overexpression of NTRK receptors, co-receptor, and ligands, within a large cohort of pan-cancer samples (Figs 1 and 2). The number of samples with comprehensive data for this analysis was 11,621 (9,966 adults and 1,655 children).

Fig 2.

Distribution of molecular alterations leading to the hyperactivation of the neurotrophic-tropomyosin receptor tyrosine kinase (NTRK) signaling pathway in human tumors (N = 11,621 samples with comprehensive molecular data). All samples that presented a nonsilent mutation, gene copy amplification, gene fusion, or mRNA overexpression of NTRK receptors (NTRK1, NTRK2, and NTRK3), co-receptor (p75^NTR), or ligands (nerve growth factor [NGF], brain-derived neurotrophic factor [BDNF], neurotrophin 3 [NT-3], and NT-4) were retrieved from a large adult and pediatric pan-cancer cohort (The Cancer Genome Atlas and St Jude’s PeCan databases; N = 11,621samples). Among the NTRK fusion cases (n = 31 from TCGA cohort), four cases had concomitant alteration within the genes that code the NTRK pathway members—ligands, co-receptor, and receptors—as follows: low-grade glioma, NTRK3 fusion plus NTF3 amplification (n = 1); low-grade glioma, NTRK1 fusion plus NTRK1 amplification (n = 1); glioblastoma, NTRK1 fusion plus NTRK1 amplification (n = 1); head and neck squamous cell carcinoma, NTRK3 fusion plus NTRK3 amplification (n = 1).

Alterations in NTRK Receptors and Ligands

Genomic and/or transcriptomic NTRK receptor alterations were found in 14.2% (1,648 of 11,621) of samples, with gene amplification and mRNA overexpression being the most frequent alterations. The three NTRK receptors were equally impacted, with frequencies of alterations ranging from 4.1% to 6.2%. In addition, the co-receptor p75^NTR presented one or more presumably activating alteration in almost 5% (579 of 11,621 samples) of tumors. NTRK ligands presented an alteration in 3.8% to 5.4% of samples. Transcript fusions were observed in NTRK receptor genes only, with the exception of two samples that presented one transcript fusion of BDNF ligand and one transcript fusion of p75^NTR (positive regulator of the NGF/NTRK1 machinery; Fig 2).

NTRK-Transcript Fusion Types

NTRK-transcript fusions that were observed in the pan-cancer cohort and/or described in the literature are listed in Table 3. The ETV6-NTRK3 rearrangement was the most frequently observed (0.09% of samples). This variant is a known biomarker of sensitivity to larotrectinib and entrectinib.^71,72 Variants TPM3-NTRK1 (0.04%), IRF2BP2-NTRK1 (< 0.01%), and SQSTM1-NTRK1 (< 0.01%) are also sensitive to larotrectinib; however, the sensitivity of the remaining 22 unique variants observed in the pan-cancer cohort is not currently known. Nine rearrangements previously described in the literature were not found in the TCGA and St Jude PeCan databases (Table 3).

Table 3.

NTRK Alterations, Frequency in TCGA/St Jude PeCan Databases, and Clinical Response to Illustrative NTRK-Targeting Inhibitors

NTRK Point Mutations

Several point mutations are acquired resistant variants to first-generation NTRK inhibitors (larotrectinib or entrectinib), but not to LOXO-195, specifically designed to overcome secondary resistance. These variants, namely NTRK1 G595R, NTRK1 G667C, NTRK3 G696A, and NTRK3 G623R, were not observed in any of the 13,467 combined adult and pediatric tumors reviewed (treatment-naïve samples; Table 3).

Co-Alterations Observed in NTRK Fusion-Positive Adult Tumor Samples

Among 31 adult tumors presenting NTRK fusions, 61.3% (19 of 31) harbored one or more co-alteration that activated the downstream PI3K signaling pathway; 58.1% (18 of 31) harbored one or more co-alteration within cell-cycle[en]associated genes; 58.1% (18 of 31) harbored one or more co-alteration within other tyrosine kinase receptors; 32.2% (10 of 31) harbored one or more co-alteration within the MAPK signaling pathway; and 35.5% (11 of 31) harbored one or more co-alteration within TP53-associated genes. NF2-activating mutations were associated with NTRK fusions in 42% (13 of 31) of samples, and TP53 (10 of 31), RB1 (six of 31) and CDKN2A (five of 31) occurred in more than 15% of the NTRK fusion–positive samples (Fig 3 and Appendix Table A1). (Adequate data to comprehensively assess co-alteration data in children was not available.) Samples bearing NTRK fusions were significantly associated with NTRK mRNA overexpression compared with samples without the fusion (Appendix Fig A1). Moreover, tumors with NTRK fusions were significantly associated with lower tumor mutational burden compared with the fusion-negative cases (Appendix Fig A2).

Fig 3.

Co-alterations associated with neurotrophic-tropomyosin receptor tyrosine kinase (NTRK) fusions in adult tumors (from The Cancer Genome Atlas). All samples that presented a gene fusion of NTRK receptors—NTRK1, NTRK2, and NTRK3—were retrieved from a large adult pan-cancer cohort (The Cancer Genome Atlas database; n = 9,966 samples). Among 31 patients with NTRK fusions, some patients also harbored co-alterations that can lead to tumorigenesis. Those co-alterations include TP53-associated genes, cell cycle–associated genes, tyrosine kinase families, and mitogen-activated protein kinase (MAPK) and phosphoinositide-3-kinase (PI3K) signaling alterations.

DISCUSSION

Along with recent advances in sequencing technology, a histology-agnostic, matched, targeted approach has emerged as a newer strategy by which to manage malignancies.^80-84 Targeting activated gene mutations or amplification/overexpression has demonstrated some remarkable successes—for example, targeting of KIT mutations for GI stromal tumors and targeting of EGFR mutation for non–small-cell lung cancer, BRAF V600E mutation for melanoma and lung cancer, and human epidermal growth factor receptor 2 overexpression for breast cancer—although in some cases the responses may be short lived.^85-91 Tumor heterogeneity and co-alterations result in resistance to targeted therapeutics.⁹² Thus, for many cancers, combination therapy may be necessary.^93-96

In some instances, targeting fusions—even with monotherapy—has shown more marked antitumor activity than targeting other alteration types. Examples include the suppression of aberrant Bcr-Abl kinase enzymatic activity that characterizes chronic myeloid leukemia. Exploitation of imatinib, dasatinib, or nilotinib leads to near-universal responses, and life expectancy increases from approximately 5 years before the imatinib era to a near-normal life span currently; however, it is also conceivable that, in this case, the success of Bcr-Abl–targeted agents is attributable to their deployment in patients with newly diagnosed disease, as advanced chronic myeloid leukemia responds poorly to single-agent Bcr-Abl kinase inhibitors.^97,98 Conversely, in patients with advanced non–small-cell- lung cancer, targeting ALK fusions demonstrates a median progression-free survival of 25.7 months with an 83% response rate, and targeting the ROS1 fusion demonstrates a median progression-free survival of 19.2 months with an approximate 70% response rate.^99,100 In addition, larotrectinib, an NTRK inhibitor, resulted in a 76% response rate in patients with an NTRK fusion.²³ These observations indicate that certain fusions act as strong drivers of tumorigenesis in specific cancers that are likely addicted to this type of founder alteration.

We reviewed data from 13,467 tumor samples in the TCGA (adult tumors) and St Jude PeCan (pediatric tumors) databases and found NTRK fusions in 0.3% of pan-cancer tumors (Table 1). Although the prevalence of these alterations is low, NTRK fusions are more often found in specific and rare tumors, such as mammary-analog secretory carcinoma of the salivary gland (93% to 100% of tumors presenting an ETV6-NTRK3 fusion), secretory breast carcinoma (ETV6-NTRK3 fusions in 92% of tumors), infantile congenital fibrosarcoma (ETV6-NTRK3 fusions in 86% to 91% of tumors), and pediatric non-brainstem high-grade glioma^14-21 (40% of tumors presenting an NTRK fusion; Table 1).

Of importance, various NTRK inhibitors are in clinical development and have differential activities (Table 2). Drugs with established clinical trial data and the ability to affect NTRK1, NTRK2, and NTRK3 fusions at low IC₅₀ include, but are not limited to, larotrectinib (76% response rate in diverse malignancies bearing NTRK fusions) and entrectinib, which also affects ALK and ROS1 rearrangements (79% response rate), and some of these responses are durable and occur with remarkable speed^22,23 (Table 2). Of interest, 32 molecules have demonstrated inhibition activity against one or more NTRK receptor (Table 2). Furthermore, five of these small inhibitors are already approved by the FDA for other indications: cabozantinib (IC₅₀ against NTRK2, 7 nM), crizotinib (IC₅₀ against NTRK1 and NTRK2, 1 nM), midostaurin (IC₅₀ against NTRK1, -2, and -3 ranging from 11 to 51 nM), nintedanib (IC₅₀ against NTRK1, -2, amd -3 ranging from 17 to 264 nM), and regorafenib (IC₅₀ against NTRK1, 74 nM). Even so, it is not known whether these five medications have anti-NTRK activity in patients. Multiple other molecules that target NTRK are also in clinical trials (Table 2).

Resistance to NTRK inhibitors is now emerging. NTRK mutations that are associated with larotrectinib or entrectinib resistance include NTRK1 F589L G595R, G667C, G667S, V573M, and NTRK3 G696A, G623R (Table 3). (These mutations were not detected in TCGA, likely because these patients had not been previously treated with NTRK inhibitors.) The resistant alterations are targetable with LOXO-195, a next-generation, selective NTRK inhibitor with promising preliminary clinical activity⁵⁰ (Fig 2). Other mechanisms of resistance may include the presence or emergence of genomic co-alterations. In the current study, NTRK-associated co-alterations were commonly discerned in genes that are involved in PI3K signaling (61% of patient samples), tyrosine kinase families (58% of patient samples), cell-cycle machinery (58% of patient samples), and MAPK pathways (32% of patient samples; Fig 3 and Appendix Table A1). Moreover, cases with NTRK fusions were significantly associated with NTRK mRNA overexpression (Appendix Fig A1), which is consistent with a previous report.¹⁰¹ Of interest, in the adult cohort, NTRK fusion–positive samples were significantly associated with a lower mutational burden compared with fusion-negative tumors (P < .001; Appendix Fig A2). This observation echoes a previous report that demonstrated that tumors harboring a driver fusion tend to have a lower number of point mutations.¹⁰¹ In contrast, high microsatellite unstable metastatic colorectal tumors have been shown to preferentially bear ALK, ROS1, or NRTK fusions.¹⁰² In our cohort, three NTRK fusion–positive colon cancer samples were observed and two of them presented with microsatellite instability-high status (data not shown). Finally, we found that nonfusion NTRK gene alterations, such as mutation, amplification, and mRNA overexpression, were found in approximately 14% of pan-cancer samples (Fig 2). Nonfusion NTRK alterations have not yet demonstrated druggability.

There are several limitations to the current study. First, clinical correlation with disease outcome among patients with NTRK alterations was not feasible because the data were not fully clinically annotated. Second, the possibility of sample size bias cannot be excluded because the number of tumor cases depended on the number of specimens submitted by investigators. Third, direct comparison between the TCGA and St Jude PeCan databases is challenging as a result of the use of different sequencing methods. Finally, we did not observe NTRK fusions in a number of cancer types, which may be a result of low sample size. Despite these limitations, the current report provides a comprehensive portrait of the genomic landscape of NTRK alterations among pan-cancer tumors using large databases.

In conclusion, NTRK fusions were observed in 0.31% (31 of 9,966) of adult tumors and 0.34% (12 of 3,501) of pediatric cancers, mostly in NTRK3 (0.16% of adult tumors) and NTRK1 (0.14% of pediatric tumors); however, some tumor types had more frequent NTRK fusions (Table 1). Additional genomic and transcriptomic NTRK alterations—mutation, amplification, and mRNA overexpression—occurred in 14.2% of samples. Genomic co-alterations were commonly observed in NTRK fusion–positive cancers, including in genes involved in PI3K signaling, tyrosine kinase families, cell-cycle–associated regulators, and the MAPK pathway (Fig 3). Additional investigation is needed to elucidate whether these genes mediate resistance to NTRK inhibition and if co-targeting them augments anti-NTRK antitumor activity. Furthermore, it would be of interest to determine whether the salutary effects of NTRK inhibitors in patients who harbor cancers with NTRK fusions can be extended via rational compound design to any of the more common NTRK alterations, such as mutation, amplification, and overexpression. Finally, the rarity of NTRK fusions, but their remarkable tractability in multiple cancer types, further expands the paradigm of tissue-agnostic genomic drug development.

Appendix

Fig A1.

Association of neurotrophic-tropomyosin receptor tyrosine kinase (NTRK) fusions and NTRK mRNA overexpression in adult tumors.

Fig A2.

Association of neurotrophic-tropomyosin receptor tyrosine kinase (NTRK) fusions and mutational burden in adult tumors. The mutational burden corresponds to the total number of nonsynonymous mutations detected by whole-exome sequencing in each sample.

Table A1.

Details of Co-Alterations With NTRK Fusions in Adult Tumors From The Cancer Genome Atlas

AUTHOR CONTRIBUTIONS

Conception and design: Ryosuke Okamura, Amélie Boichard, Razelle Kurzrock

Collection and assembly of data: Ryosuke Okamura, Amélie Boichard

Data analysis and interpretation: All authors

Manuscript writing: All authors

Final approval of manuscript: All authors

Accountable for all aspects of the work: All authors

AUTHORS' DISCLOSURES OF POTENTIAL CONFLICTS OF INTEREST

The following represents disclosure information provided by authors of this manuscript. All relationships are considered compensated. Relationships are self-held unless noted. I = Immediate Family Member, Inst = My Institution. Relationships may not relate to the subject matter of this manuscript. For more information about ASCO's conflict of interest policy, please refer to www.asco.org/rwc or ascopubs.org/po/author-center.

Ryosuke Okamura

No relationship to disclose

Amélie Boichard

No relationship to disclose

Shumei Kato

No relationship to disclose

Jason K. Sicklick

Consulting or Advisory Role: BioTheranostics, Loxo Pharmaceuticals, Grand Rounds

Research Funding: Novartis, Foundation Medicine, Blueprint Medicines

Lyudmila Bazhenova

Stock and Other Ownership Interests: Epic Sciences

Consulting or Advisory Role: AstraZeneca, Novartis, Ariad Pharmaceuticals, Genentech, Bristol-Myers Squibb

Speakers' Bureau: Genentech, AstraZeneca, Pfizer

Research Funding: BeyondSpring Pharmaceuticals

Razelle Kurzrock

Leadership: CureMatch

Stock and Other Ownership Interests: CureMatch

Consulting or Advisory Role: Sequenom, Actuate Therapeutics, Loxo Pharmaceuticals, NeoMed, Xbiotech

Speakers' Bureau: Roche

Research Funding: Guardant Health (Inst), Sequenom (Inst), Merck Serono (Inst), Genentech (Inst), Pfizer (Inst), Foundation Medicine (Inst), Incyte (Inst), Konica Minolta (Inst)