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. 2015 Oct 14;20(10):18685–18703. doi: 10.3390/molecules201018685

Figure 4.

Figure 4

Flow cytometry histograms derived from scatter plots showing fluorescence of live (<103) or dead (>103) of S. mutans stained with PI post 24 h incubation with controls or extracts. X-axis corresponds to the PI positive marked microorganisms and Y-axis to the total cell count. (A) S. mutans sample PI negative control, 99.8% viability; (B) S. mutans incubated for 30 min with ethanol 70% PI positive control 30.6% viability; (C) water dry leaf non-boiled extract 1 μg/mL, 1.5% viability; (D) Acetone 4 μg/mL, 0.5% viability; and (E) Petroleum ether 1 μg/mL, 41.6% viability; (F) Chloroform 3.5 μg/mL, 0.3% viability.