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. 2015 Sep 11;20(9):16491–16523. doi: 10.3390/molecules200916491

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© 2015 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Fluorescence spectroscopy of compounds 1–4 and BSA. Numbers 1–13 indicated concentrations of compounds 1–4: 0.0, 0.4 ×10⁻⁶, 0.8 × 10⁻⁶, 1.2 × 10⁻⁶, 2.4 × 10⁻⁶, 3.6 × 10⁻⁶, 4.8 × 10⁻⁶, 6.0 × 10⁻⁶, 7.2 × 10⁻⁶, 8.4 × 10⁻⁶, 9.6 × 10⁻⁶, 10.8 × 10⁻⁶ and 12 × 10⁻⁶ mol∙L⁻¹, respectively. BSA concentration applied was 1.04 × 10⁻⁶ mol∙L⁻¹. Scan condition: Ex = 280 nm, Em = 290–550 nm; slits of both Ex and Em of compounds 1–3 were 5 nm while those of compound 4 were 5 nm and 10 nm, respectively.

Fluorescence spectroscopy of compounds 1–4 and BSA. Numbers 1–13 indicated concentrations of compounds 1–4: 0.0, 0.4 ×10⁻⁶, 0.8 × 10⁻⁶, 1.2 × 10⁻⁶, 2.4 × 10⁻⁶, 3.6 × 10⁻⁶, 4.8 × 10⁻⁶, 6.0 × 10⁻⁶, 7.2 × 10⁻⁶, 8.4 × 10⁻⁶, 9.6 × 10⁻⁶, 10.8 × 10⁻⁶ and 12 × 10⁻⁶ mol∙L⁻¹, respectively. BSA concentration applied was 1.04 × 10⁻⁶ mol∙L⁻¹. Scan condition: Ex = 280 nm, Em = 290–550 nm; slits of both Ex and Em of compounds 1–3 were 5 nm while those of compound 4 were 5 nm and 10 nm, respectively.