Figure 4.

Cytoprotective effect of P. major hydroethanolic extract against t-BOOH-induced cell death. HepG2 cells (1.6 × 10⁵ cells/cm²) were incubated for 24 h in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum at 37 °C in a 5% CO₂ atmosphere with 0.25 or 0.5 mM t-BOOH in the presence (gray bars) and absence (black bars) of 0.1 mg/mL PmHE. Both, t-BOOH and PmHE were added at the same time. */** Statistically different from 0.25 and 0.5 mM t-BOOH, respectively (p < 0.05).