Table 1.
Purification of Tricholoma matsutake α-galactosidase.
| Chromatographic Fraction | Total Protein (mg/400 g) | Total Activity (U) a | Specific Activity (U/mg) b | Yield (%) | Purification Fold c |
|---|---|---|---|---|---|
| Crude extract | 17,427 | 116,204 | 6.7 | 100 | 1 |
| Q2 | 311.7 | 102,037 | 327.3 | 87.9 | 49.1 |
| MonoQ1 | 95.6 | 34,490 | 360.8 | 29.7 | 54.1 |
| SU2 | 9.6 | 8727 | 909.1 | 7.5 | 136.3 |
a Total activity: α-galactosidase activity (U/mL) in each step × Volume (mL); b Specific activity: total activity/Total protein; c Purification fold: specific activity of each step/specific activity of the first step.