Table 3.

Stability of scopoletin in rat plasma under different storage conditions (n = 5).

Storage Conditions	Nominal Con. (ng/mL)	Measured Con. (Mean ± SD, ng/mL)	RSD (%)	RE (%)
3 freeze-thaw cycles	10	10.36 ± 0.57	5.5	3.6
	100	101.66 ± 3.99	3.9	1.7
	800	804.58 ± 6.13	0.8	0.6
Short-term stability for 6 h (25 °C)	10	9.82 ± 0.49	5.0	−1.8
	100	101.39 ± 5.27	5.2	1.4
	800	812.33 ± 30.27	3.7	1.5
Long-term stability for 1 month (−20 °C)	10	9.30 ± 0.45	4.8	−7.0
	100	98.20 ± 3.59	3.7	−1.8
	800	813.78 ± 21.83	2.7	1.7
Long-term stability for 2 weeks (4 °C)	10	9.80 ± 0.43	4.4	−2.0
	100	99.84 ± 2.97	3.0	−0.2
	800	812.69 ± 21.48	2.6	1.6
Autosampler for 24 h (25 °C)	10	9.83 ± 0.55	5.6	−1.7
	100	97.62 ± 4.40	4.5	−2.4
	800	805.89 ± 24.31	3.0	0.7

Abbreviations: con., concentration.