Fig. 3.

Microbial functional profiles of T- and M-metatranscriptomes. PCA for eggNOG functional categories (a) and expressed genes (b), which were performed based on auto-scaled abundances (cpm) of functional features. Correlations between rumen T- and M-metatranscriptomes were calculated using Spearman’s rank correlation based on functional categories (c) and expressed genes (d). Each scatterplot in c and d illustrates log₁₀-transformed mean abundances (cpm) of each functional category and each expressed gene. e Cluster analysis showing that between-method variations were lower than between-subject variations, which was conducted based on auto-scaled abundances (cpm) of functional categories using Euclidean as distance measure and Ward as clustering method