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. 2019 Jan 3;8:e40063. doi: 10.7554/eLife.40063

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© 2019, Miller et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 5. — (A) Lysate from M. smegmatis ∆secA2/∆satS expressing SapM-FLAG and either SatS_Mtb-HA or SatS_Mtb without a tag, ∆satS expressing ∆ss-SapM-FLAG and SatS_Mtb-HA, or mc²155 with two empty vectors (as shown above the blot) were used for co-immunoprecipitation using anti-HA conjugated beads. Lysates (left) and immunoprecipitations (right) for each strain were probed with SatS antibody and FLAG antibody for SapM. Two different sizes of SapM-FLAG corresponding to the full-length (signal sequence-containing) and mature (cleaved signal sequence) species were detected. (B) Lysate from M. smegmatis ∆satS expressing SapM-FLAG and either SatS_Mtb-HA or SatS_Mtb without a tag were used for co-immunoprecipitation using anti-HA conjugated beads. Lysates (left) and co-immunoprecipitations (right) for each strain were probed with SatS antibody, FLAG antibody, and MspA antibody. All data are representative of at least three independent experiments.

Figure 5—figure supplement 1. — (A) Lysate from M. smegmatis ∆secA2/∆satS expressing SapM-FLAG and either SatS_Mtb-HA or SatS_Mtb without a tag, ∆satS expressing ∆ss-SapM-FLAG and SatS_Mtb-HA, or mc²155 with two empty vectors (as shown above the blot) were used for co-immunoprecipitation using anti-HA conjugated beads. Lysates (left) and immunoprecipitations (right) for each strain were probed with SatS antibody and FLAG antibody for SapM. Two different sizes of SapM-FLAG corresponding to the full-length (signal sequence-containing) and mature (cleaved signal sequence) species were detected. (B) Lysate from M. smegmatis ∆satS expressing SapM-FLAG and either SatS_Mtb-HA or SatS_Mtb without a tag were used for co-immunoprecipitation using anti-HA conjugated beads. Lysates (left) and co-immunoprecipitations (right) for each strain were probed with SatS antibody, FLAG antibody, and MspA antibody. All data are representative of at least three independent experiments.