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Korean Journal for Food Science of Animal Resources logoLink to Korean Journal for Food Science of Animal Resources
. 2018 Dec 31;38(6):1261–1272. doi: 10.5851/kosfa.2018.e62

Effects of Queso Blanco Cheese Containing Bifidobacterium longum KACC 91563 on the Intestinal Microbiota and Short Chain Fatty Acid in Healthy Companion Dogs

Ho-Eun Park 1,†, Ye Jin Kim 2,†, Kyung-Hyo Do 1, Jae Kwang Kim 2, Jun-Sang Ham 3, Wan-Kyu Lee 1,*
PMCID: PMC6335144  PMID: 30675119

Abstract

The effects of Queso Blanco cheese containing Bifidobacterium longum KACC 91563 was studied on the intestinal microbiota and short chain fatty acids (SCFAs) in healthy companion dogs. There were three experimental groups with five healthy dogs each: a control group, not fed with any cheese, and groups fed with Queso Blanco cheese with (QCB) or without B. longum KACC 91563 (QC) for 8 weeks. Fecal samples were collected 5 times before, during, and after feeding with cheese. Intestinal microbiota was analyzed using two non-selective agar plates (BL and TS) and five selective agar plates (BS, NN, LBS, TATAC, and MacConkey). SPME-GC-MS method was applied to confirm SCFAs and indole in dog feces. The six intestinal metabolites such as acetic, propionic, butyric, valeric, isovaleric acid and indole were identified in dog feces. Administration of B. longum KACC 91563 (QCB) for 8 weeks significantly increased the beneficial intestinal bacteria such as Bifidobacterium (8.4±0.55) and reduced harmful bacteria such as Enterobacteriaceae and Clostridium (p<0.05). SCFA such as acetic and propionic acid were significantly higher in the QCB group than in the Control group (p<0.05). In conclusion, this study demonstrates that administration of Queso Blanco cheese containing B. longum KACC 91563 had positive effects on intestinal microbiota and metabolites in companion dogs. These results suggest that Queso Blanco cheese containing B. longum KACC 91563 could be used as a functional food for companion animals and humans.

Keywords: probiotics, Queso Blanco cheese, Bifidobacterium longum, KACC 91563, dog

Introduction

Intestinal microbiota plays a crucial role in the health of the host from humans to animals. The intestinal microbiota consist of about 1,000 different microbial species, and the intestinal tract are microbial habitats with a high population density (Qin et al., 2010; Tannock, 2001). The composition and metabolic activity of microorganisms in the intestines are affected by diet, age, stress and antibiotics et al. (Biagi et al., 2010). Furthermore, intestinal flora provides nutrient substrate, affects the immune system, and prevents the multiplication of intestinal pathogens (Hooper et al., 2001). The regulation of human intestinal flora has attracted considerable scientific and commercial interests, and probiotics have been widely used to improve intestinal health.

Probiotics are live microbial additives that beneficially influence the host by maintaining the balance of healthy intestinal microbiota (Fuller, 1989). Probiotics are known to improve intestinal health through a variety of mechanisms, including intestinal pathogen displacement, production of antimicrobial substances, and immune modulating response (George Kerry et al., 2018; Lee et al., 2003). The most commonly used probiotics are lactic acid bacteria such as Bifidobacterium and Lactobacillus. They are also known to be representative beneficial intestinal bacteria (Holzapfel et al., 1998).

Bifidobacterium are gram positive, anaerobic, non-spore-forming, and non-motile bacteria (Prasanna et al., 2014). They play an important role in humans and account for about 3% to 7% of adult microbial bacteria (Lahtinen et al., 2011). They are known to possess many function such as production of antimicrobial agents, reduction of serum cholesterol level, synthesis of vitamin B, enhancement of immune system, and anti-carcinogenic activity (Martinez et al., 2013). Bifidobacterium also improves intestinal health by controlling the microbial activity in the gastrointestinal tract (Marteau et al., 2002). It was reported that the intake of Bifidobacterium infantis aids in the treatment of irritable bowel syndrome (O'Mahony et al., 2005). Furthermore, Lee et al. (1999) found that intake of B. longum HY8001 by healthy people improved their intestinal microbial composition and had a protective effect against colon cancer (Lee et al., 1999).

Composition and metabolic activity of intestinal microbiota affect intestinal SCFAs. SCFA is an essential component of bowel health and is associated with a variety of health conditions (Gibson and Roberfroid, 1995). In addition to functioning as an energy source, SCFA improves the risk of metabolic syndrome, promotes intestinal motility, regulate immune system and reduces plasma cholesterol (Hemalatha et al., 2017). It is known that lactic acid bacteria (LAB) such as Bifidobacterium and Lactobacillus participate in the production of SCFA (den Besten et al., 2013). Several studies demonstrated the effects of LAB on the intestinal health in companion dogs (Baillon et al., 2004; Biagi et al., 2007; Strompfova et al., 2014). In this experiment, changes in intestinal LAB and SCFA were simultaneously analyzed.

Cheese is a good source of protein, calcium, and vitamins for dogs. Even if cheese has a bad reputation because of high energy or salts, eating small amounts of cheese shouldn’t be a problem in healthy dogs. The majority of cheeses is safe for the majority of dogs. Queso Blanco cheese is one of the easier cheeses to make and does not requires careful handling. Also, it is not difficult to add B. longum technically. In this experiment, Queso Blanco cheese containing B. longum was used for the first time to confirm the improvement of intestinal health in companion dogs. Therefore, the purpose of this experiment is to investigate whether the administration of cheese containing B. longum KACC 91563 improves intestinal microbiota and SCFA by controlling the composition and metabolic activity of intestinal microbiota in the healthy companion dogs.

Materials and Methods

Preparation of cheese

Queso Blanco cheese was made according to Ham et al. (2007) with some modification. Four hundreds grams of citric acid in 20 L of water was added to 100 kg of skimmed milk, and salt was not added. B. longum KACC 91563 for QCB cheese was added before molding. Each cheese was stored at 4℃ and fed in a month (QC Moisture 54.7%–55.1%, Protein 36.9%–37.4%; QCB Moisture 53.6%–54.2%, Protein 37.1%–37.5%). The daily intake of B. longum cheese (5.0×108 CFU/10 g cheese) for dogs was maintained at 10 g/kg of body weight on a daily basis for 8 weeks.

Cheese composition

Moisture and protein contents were analyzed using a Food Scan (Food ScanTM Lab 78810, Foss Tecator Co. Ltd., Denmark) according to the method suggested by the Association of Official Analytical Chemists (AOAC, 2006).

Animals and experimental design

Fifteen healthy companion dogs (6 females and 9 males) in the Veterinary Medical Center at Chungbuk National University (Cheongju, Korea) were chosen and randomly divided into 3 experimental groups of 5 dogs each. All animal experiments were approved by the Institutional Animal Care and Use Committee of Chungbuk National University (Approval no. CBNUA-1104-17-01). The dogs were 1 to 13 years old, and the body weight range was 2.5 to 35 kg. Breeds included Bulldog, Welsh Corgi, Dachshund, Yorkshire Terrier, Silky Terrier, Poodle, Shih Tzu, Labrador Retriever, Spitz, Cocker Spaniel, and Miniature Pinscher. QCB group was fed with 10 g of Queso Blanco cheese containing B. longum KACC 91563 and QC group was fed with 10 g of Queso Blanco cheese devoid of B. longum KACC 91563 daily for 8 weeks per kg of body weight. A control group (Control) was not fed with any kind of cheese. Fresh fecal samples were collected 5 times, before intake of cheese (week –2 and week 0), during cheese intake (week 4 and week 8), and after cheese intake (week 10), respectively.

Analysis of the intestinal microbiota

Intestinal microbiota analysis was performed by the Mitsuoka method (Mitsuoka et al., 1969). Two different non-selective agar plates, BL for anaerobes and TS for aerobes, and 5 selective agar plate, MacConkey (Escherichia coli and Salmonella spp.), TATAC (Enterococcus spp., and Streptococcus spp.), LBS (Lactobacillus spp.), BS (Bifidobacterium spp.), and NN (Clostridium spp.) were used for detection of intestinal microbiota. BL, LBS, BS, and NN agar plates were anaerobically incubated at 37℃ for 48 h in an anaerobic jar using the steel wool method (Mitsuoka et al., 1969). TS and MacConkey agar plates were incubated aerobically at 37°C for 48 h. After incubation, each plate was examined for bacterial colonies. The colonies were identified based on their morphology, gram staining, spore formation, aerobic growth and 16S rRNA gene sequence. The number of bacteria per gram of stool was calculated and was converted to a logarithmic equivalent (Mitsuoka et al., 1969).

Chemicals for short chain fatty acids analysis

The acetic acid (≥99% purity), propionic acid (≥99% purity), butyric acid (≥99% purity), valeric acid (≥98% purity), isovaleric acid (≥99% purity), 2-methylvaleric acid (internal standard, IS) (≥98% purity) and indole (≥99% purity) were purchased from Tokyo Chemical Industry (Tokyo, Japan).

Headspace solid-phase microextraction (HS-SPME) analysis of feces

Analysis of the volatile compounds by HS-SPME method was modified from previously described method (Bianchi et al., 2011). The feces sample (50 mg) was mixed with 280 μL of methanol, 20 μL of IS (5 mg/mL) and 30 μL of 0.9 M H2SO4 solution in 20 mL climp vial (Shimadzu, Tokyo, Japan). The vial was immediately covered with a vial cap. The sample vial was heated on hot plate for 30 min at 60℃. At the same time, a SPME fiber (50/30 μm divinylbenzene/Carboxen/ polydimethylsiloxane (2 cm); No. 57348-U, Supelco Inc., PA, USA) was pre-conditioned using SPME fiber conditioner (Field forensics, Inc., FL, USA) for 15 min at 250℃. After sample conditioning, the fiber was inserted into headspace. The volatile compounds were concentrated on fiber for 15 min at 60℃. The fiber was injected in injector hole. The volatile compounds were detached from the fiber and analyzed by using gas chromatography coupled with quadrupole mass spectrometer (GC-qMS). The GC-qMS was performed with GCMS-QP2010 Ultra system and auto sampler AOC-20i (Shimadzu). The volatile compounds were separated in equipped DB-5 column (0.25 mm×0.25 μm×30 m; Agilent, Palo Alto, CA, USA). The helium was used as carries gas, and column flow rate was 1.3 mL/min. The injection, interface, and ion source temperatures were 250℃, 260℃, and 250℃, respectively. The column program was as follows: initial temperature was 60℃ for 3 min. Next, temperature was increased to a rate of 40℃/min up to 260℃, and maintained for 5 min. In full scan mode, the scanned mass range was 30–250 m/z. Selected ion monitoring was performed by monitoring 43 m/z for acetic acid, 60 m/z for butyric, valeric and isovaleric acid, 74 m/z for propionic and 2-methylvaleric acid, and 117 m/z for indole. Split ratio was 10:1. The mass spectra were analyzed using the Lab solutions GC-MS solution software version 4.11 (Shimadzu). Peak identification was based on comparison with retention time and mass spectra fragmentation of standard. Quantitative analysis of volatile compounds was proportionally calculated with ratio of the standard to IS peak area.

Statistical analysis

Data was analyzed using SPSS 12.0 for Windows (IBM, USA). Significant differences between the groups were tested by Student’s t test or ANOVA (p<0.05). To demonstrate difference between groups partial least-squares discriminant analysis (PLS-DA) was performed using SIMCA-P version 14.1 (Umetrics, Umea°, Sweden). The significant differences of metabolites (p<0.05) were determined with Student's t-test using Metaboanalyst (www.metaboanalyst.ca). The box plot also was obtained within Metaboanalyst.

Results and Discussion

Analysis of the intestinal microbiota

The composition and metabolic activity of intestinal microbiota have a great influence on the health of the host. Probiotics are widely used because they regulate beneficial bacteria and harmful bacteria in the intestines to maintain healthy intestines (Butel, 2014). Effects of Queso Blanco cheese containing B. longum KACC 91563 on the composition of intestinal microbiota of healthy companion dogs are shown in Table 1, 2, and 3. Before administration of Queso Blanco cheese (–2 and 0 week), Enterococcus (7.8±1.95 to 9.4±1.23) and Lactobacillus (8.9±0.79 to 9.8±0.76) were the major LAB, but Bifidobacterium was not detected in all groups before administration of Queso Blanco cheese, and there was no significant difference in total bacterial counts among three groups (Table 1). At 4 and 8 weeks, Bifidobacterium was not detected in either the control or the QC groups (Table 2). However, Bifidobacterium was only detected on the 8 week after the administration of the cheese containing B. longum in the QCB group (8.4±0.55). And Enterobacteriaceae was significantly decreased to 7.2±0.92 than that of QC and Control group. The increase of Bifidobacterium and decrease of Enterobacteriaceae were considered due to administration of Queso Blanco cheese containing B. longum. The intake of probiotics is known to increase the number of lactobacilli and bifidobacteria in the small intestine (Brigidi et al., 2001). Similarly, the administration of B. animalis B/12 significantly increased the number of LAB in healthy dogs compared to the control group (Strompfova et al., 2014). Matsumoto et al. (2000) and Bartosch et al. (2005) reported that the intake of Bifidobacterium spp. in the elderly increased the frequency of defecation and the number of intestinal Bifidobacterium spp. in the intestines (Bartosch et al., 2005; Matsumoto et al., 2000). Changes in the frequency and number of Bifidobacterium in the intestines have been used as a major index for the improvement of intestinal microbial counts.

Table 1. Intestinal microbiota of dogs before administration of Queso Blanco cheese.

Bacterial group –2 week 0 week
QCB1) QC Control QCB QC Control
Enterobacteriaceae 8.6±1.372)(100)3) 8.0±0.82(100) 9.2±0.69 (100) 8.4±0.76(100) 8.5±0.14(100) 9.2±0.42(100)
Enterococcus 7.9±1.86(100) 7.8±1.95(100) 9.2±1.24(100) 8.9±0.88(100) 8.7±0.73(100) 9.4±1.23(100)
Yeast -4) - 2.8±0.00(20) - - -
Lactobacillus 9.6±0.30(80) 9.8±0.76(80) 8.9±0.79(80) 9.1±0.44(80) 9.0±0.52(80) 9.0±0.68(100)
Bifidobacterium - - - - - -
Eubacterium - 8.1±0.00(20) 9.7±0.00(20) - 8.3±1.39(60) 9.1±0.00(20)
Bacteroidaceae 9.8±0.34(100) 9.1±0.46(100) 9.4±0.52(100) 9.7±0.68(100) 9.5±0.91(100) 9.0±0.49(100)
Clostridium spp. 3.6±0.43(40) 6.8±1.24(60) 4.1±2.31(60) 4.9±0.00(20) 7.2±2.95(40) 3.8±2.55(60)
Total counts 10.2±0.19 9.7±0.69 10.2±0.22 9.9±0.69 9.9±0.52 10.0±0.45
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1) QCB, Queso Blanco cheese with B. longum KACC 91563; QC, Queso Blanco cheese without B. longum KACC 91563; C, control.

2) Data expressed as mean±log10 count of bacteria/g of feces.

3) Figures in parentheses corresponds to frequency of occurrence (%).

4) -, Not detected.

Table 2. Intestinal microbiota of dogs during administration of Queso Blanco cheese.

Bacterial group 4 week 8 week
QCB1) QC Control QCB QC Control
Enterobacteriaceae 7.6±1.772) (100)3) 8.2±1.57(100) 7.9±1.48(100) 7.2±0.92(100)a 8.4±0.64(100)b 8.7±0.37(100)b
Enterococcus 8.1±2.10(100) 8.9±0.74(100) 9.3±0.58(100) 9.4±0.73(100) 8.8±0.93(100) 7.5±1.54(100)
Yeast -4) - - - - -
Lactobacillus 4.8±3.05(60) 4.1±3.05(60) - 6.4±3.37(60) 7.0±3.03(60) 4.8±2.25(40)
Bifidobacterium - - - 8.4±0.55(60) - -
Eubacterium - - - - - -
Bacteroidaceae 10.0±0.54(100) 9.2±1.44(80) 9.3±0.62(100) 9.8±0.99(100) 9.3±0.89(100) 9.1±0.66(100)
Clostridium spp. 2.7±0.00(20) 2.0±0.00(20) 6.2±0.00(20) 2.7±0.66(40)
Total counts 10.2±0.43 9.5±1.26 9.7±0.49 10.4±0.32 9.7 ±0.38 9.4 ±0.51
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1) QCB, Queso Blanco cheese with B. longum KACC 91563; QC, Queso Blanco cheese without B. longum KACC 91563; C, control.

2) Data expressed as mean±log10 count of bacteria/g of feces.

3) Figures in parentheses corresponds to frequency of occurrence (%).

4) -, Not detected.

ab Different superscript letters indicate the statistical differences determined by ANOVA (p<0.05).

Table 3. Intestinal microbiota of dogs after administration of Queso Blanco cheese.

Bacterial group 10 week
QCB1) QC Control
Enterobacteriaceae 7.9±1.082) (100)3)a 8.8±0.30(100)ab 9.3±0.37(100)b
Enterococcus 8.6±0.90(100) 9.5±0.62(100) 8.6±0.74(100)
Yeast -4) - -
Lactobacillus 5.9±0.40(60) 6.9±3.68(60) 7.6±0.00(20)
Bifidobacterium 7.8±0.97(40) - -
Eubacterium - - -
Bacteroidaceae 9.6±0.90(100) 9.7±0.79(100) 9.5±0.60(100)
Clostridium spp. - 4.7±0.00(20) 3.6±2.30(40)
Total counts 9.8±0.69 10.2±0.26 9.8±0.36
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1) QCB, Queso Blanco cheese with B. longum KACC 91563; QC, Queso Blanco cheese without B. longum KACC 91563; C, control.

2) Data expressed as mean± log10 count of bacteria/g of feces.

3) Figures in parentheses corresponds to frequency of occurrence (%).

4)-, Not detected.

a,b Different superscript letters indicate the statistical differences determined by ANOVA (p<0.05).

Probiotics control host microbiota by limiting and preventing colonization of intestinal pathogenic bacteria. They inhibit pathogenic bacteria by producing bacteriocin SCFA, hydrogen peroxide (H2O2) and diacetyl (George Kerry et al., 2018). The number of Enterobacteriaceae was significantly (p<0.05) decreased in the QCB group (7.2±0.92) compared to QC group (8.4±0.64) and control group (8.7±0.37) at 8 weeks of the cheese administration. In addition, significant (p<0.05) difference was observed in the QC group (8.8±0.30) and control group (9.3±0.37) compared with the QCB group (7.9±1.08) on number of Enterobacteriaceae at 10 weeks (Table 3). Clostridium was not detected in the QCB group during the 8 and 10 week, whereas Clostridium was isolated in the QC group (6.2±0.00 and 4.7±0.00, respectively) and the control group (2.7±0.66 and 3.6±2.30, respectively). Similarly, Baillon et al. (2004) and Biagi et al. (2007) reported that administration of LAB in adult dogs improves the composition of gastrointestinal microbial population and is highly effective in inhibiting the growth of harmful bacteria such as Enterobacteriaceae and Clostridium in the intestine (Baillon et al., 2004; Biagi et al., 2007). The O'Mahony study also showed a significant decrease in Clostridium spp. bacteria at the 6th week of administration of B. animalis AHC7. In fact, the increase in bifidobacteria is known to be accompanied by a decrease in clostridia, and it has also been confirmed in this study (Gibson et al., 1995). The total bacterial count in all groups remained relatively constant throughout the experiment (Fig. 1). No detectable changes were detected in the number and frequencies of Bacteroidaceae throughout the experiment (Table 1, 2, and 3).

Fig. 1. Effects of cheese containing B. longum (QCB) on the intestinal microbiota of healthy companion dogs.

Fig. 1

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A, Enterobacteriaceae number (expressed as Log10); B, Clostridium number (expressed as Log10); C, Bifidobacterium number (expressed as Log10); D, Total bacterial cell number (expressed as Log10). Dogs were fed Queso Blanco cheese containing B. longum KACC 91563 (QCB) for 8 weeks. Data represents the mean±SD of 5 dogs from each group. * p<0.05; compared with the control group.

Intestinal enteropathogenic microorganisms such as enterotoxigenic E. coli, Shigella, and Salmonella induce diarrhea. Intestinal microorganisms such as lactobacilli and bifidobacteria, which produce lactic acid, inhibit enteropathogenic microorganisms, thereby preventing and treating intestinal diseases (Hilton et al., 1997; Sullivan and Nord, 2002). Lee et al. (1999) has shown that administration of LGG relieves symptoms of irritable bowel syndrome (IBS) and possess a therapeutic effect against gastrointestinal disorders (Lee et al., 1999). In this experiment it was confirmed that the number of Enterobacteriaceae decreased in feces of dogs fed with cheese containing B. longum. Further studies will be needed to establish the suitability of using B. longum for the prevention of intestinal diseases using intestinal disease models.

The current study demonstrates that administration of B. longum increases beneficial intestinal bacteria and reduces harmful bacteria such as Enterobacteriaceae and Clostridium in healthy companion animals. These changes in the number of intestinal microbiota suggests that B. longum KACC 91563 may control the intestinal microorganisms that affect gut homoeostasis.

GC-MS analysis of the volatile metabolites in dog feces

The volatile compounds such as SCFAs and indole are end products generated by intestinal microbiota (den Besten et al., 2013). In addition, the volatile compounds are known as essential marker for host health in intestine (Nicholson et al., 2012). Recently, these compounds are analyzed using SPME-GC-MS method because this method allows that rapid, relatively simple and specific quantitative analysis of trace element (Goldmann et al., 2005). Therefore, in this study, SPME-GC-MS method was applied to confirm SCFAs and indole in dog feces. As a result, the six metabolites such as acetic, propionic, butyric, valeric, isovaleric acid and indole were identified in dog feces.

The multivariate analysis, such as principal component analysis (PCA) and PLS-DA are useful tool for characterizing the data in a large number of variables (Cabaton et al., 2013). The PCA as an unsupervised method sets the data by regardless of the origin of variation. However, when more than two groups are included in the analysis, PLS-DA is more appropriate than PCA (Lindon et al., 2004). The PLS-DA as a supervised method is an alternative to PCA because it predicts the data by the significant predictor variables (Kalivodova et al., 2015). In this study, PLS-DA was performed with data of metabolites obtained from week –2 to week 10 among QCB, QC and Control. As a result, the difference between the groups was only shown at 8 weeks. In score plot of PLS-DA model derived from metabolites in dog feces between QCB and Control groups, each point means feces samples fed QCB or Control diets. The two groups were separately clustered (Fig. 2A). Likewise, the biochemical compositions of QCB and QC groups were differently shown each other in score plot (Fig. 2B). In the loading plot between QCB and Control groups, acetic acid and propionic acid mainly affected the separation between two groups (Fig. 2A). Also, the two metabolites were significantly higher in the QCB group than in the Control group (p<0.05) (Fig. 3A). SCFA has an important correlation with total intestinal bacteria, lactobacilli, and bifidobacteria (den Besten et al., 2013). In general, Bifidobacterium spp. produces high levels of SCFA such as propionic acid, butyric and acetic acid and SCFA inhibits pathogens by reducing intestinal pH (Gibson et al., 2004; Roberfroid et al., 2010). The increased levels of acetic acid and propionic acid in QCB group correlated with increased levels of Bifidobacterium spp. compared with control group at 8 weeks. Similarly, Lactobacillus plantarum MA2 was found to raise the concentration of propionic acid in the feces of rat (Wang et al., 2009). Many researcher reported that the administration of probiotic cheese significantly increased the acetic acid concentration compared to the control group and confirmed by the presence of bifidobacteria (Ong et al., 2006; van Zanten et al., 2012). These results demonstrated that the increased level of acetic and propionic acids produced by bifidobacteria. Strompfova et al. (2014) also showed that the administration of B. animalis B/12 in companion dogs resulted in an increase the production of SCFA, such as acetic acid and valeric acid, while fecal coliforms decreased. In this study, Bifidobacterium was increased, while Enterobacteriacea was decreased in the QCB group compared to other groups at 8th week. These results are related to the bactericidal action of acetic acid produced by Bifidobaterium. In the loading plot of the QCB and QC groups, butyric acid was increased in QC group than in the QCB group and had only significant difference (p<0.05) (Fig. 2B and 3B). Clostridium spp. bacteria are known to produce butyric acid in cheese or milk (Brandle et al., 2016). In the QC group, Clostridium spp. was detected at the 8th week in analysis of intestinal microbiota, and this result may be related to a significant increase in the production of butyric acid. The QC group increased acetic acid production more than QCB group, but there was no significant difference.

Fig. 2. The score (left) and loading (right) plots of PLS-DA model obtained from GC-MS data in dog feces between QCB (filled square) and C (triangle, A), and QC (open square, B).

Fig. 2

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PLS-DA, partial least-squares discriminant analysis; QCB, Queso Blanco cheese with B. longum KACC 91563; QC, Queso Blanco cheese without B. longum KACC 91563; C, control.

Fig. 3. The box plots of SCFAs and indole in dog feces between QCB and Control (A), and QC (B) groups.

Fig. 3

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* p<0.05 (The selected metabolites have variable importance in the projection (VIP) value of >1.0 in the PLS-DA model and the p values (p<0.05) in the t-tests for all metabolites); QCB, Queso Blanco cheese with B. longum KACC 91563; QC, Queso Blanco cheese without B. longum KACC 91563; C, control; SCFAs, short chain fatty acids; PLS-DA, partial least-squares discriminant analysis.

Conclusions

The current study indicates that administration of B. longum KACC 91563 for 8 weeks significantly increased the beneficial intestinal bacteria such as Bifidobacterium and reduced harmful bacteria such as Enterobacteriaceae and Clostridium (p<0.05). SCFA such as acetic and propionic acid were significantly higher in the QCB group than in the Control group (p<0.05). In conclusion, this study demonstrates that administration of Queso Blanco cheese containing B. longum KACC 91563 had positive effects on intestinal microbiota and metabolites in companion dogs. These results suggest that Queso Blanco cheese containing B. longum KACC 91563 could be used as a functional food for companion animals and humans.

Acknowledgements

The first two authors Ho-Eun Park and Ye jin Kim contributed equally to this work. This work was carried out with the support of “Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ012834),” Rural Development Administration, Korea.

Footnotes

Conflicts of Interest

The authors declare no potential conflict of interest.

Ethics Approval (IRB/IACUC)

All animal experiments were approved by the Institutional Animal Care and Use Committee of Chungbuk National University (Approval no. CBNUA-1104-17-01).

References

  1. AOAC. Official methods of analysis. Association of Officaial Analytical Chemists. 15th ed. Washington, DC, USA: 2006. pp. 210–219. [Google Scholar]
  2. Baillon ML, Marshall-Jones ZV, Butterwick RF. Effects of probiotic Lactobacillus acidophilus strain DSM13241 in healthy adult dogs. Am J Vet Res. 2004;65:338–343. doi: 10.2460/ajvr.2004.65.338. [DOI] [PubMed] [Google Scholar]
  3. Bartosch S, Woodmansey EJ, Paterson JC, Mcmurdo ME, Macfarlane GT. Microbiological effects of consuming a synbiotic containing Bifidobacterium bifidum, Bifidobacterium lactis, and oligofructose in elderly persons, determined by real-time polymerase chain reaction and counting of viable bacteria. Clin Infect Dis. 2005;40:28–37. doi: 10.1086/426027. [DOI] [PubMed] [Google Scholar]
  4. Biagi E, Nylund L, Candela M, Ostan R, Bucci L, Pini E, Nikkila J, Monti D, Satokari R, Franceschi C, Brigidi P, De Vos W. Through ageing, and beyond: Gut microbiota and inflammatory status in seniors and centenarians. PLOS ONE. 2010;5:e10667. doi: 10.1371/journal.pone.0010667. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Biagi G, Cipollini I, Pompei A, Zaghini G, Matteuzzi D. Effect of a Lactobacillus animalis strain on composition and metabolism of the intestinal microflora in adult dogs. Vet Microbiol. 2007;124:160–165. doi: 10.1016/j.vetmic.2007.03.013. [DOI] [PubMed] [Google Scholar]
  6. Bianchi F, Dall'asta M, Del Ri D, Mangia A, Musci M, Scazzina F. Development of a headspace solid-phase microextraction gas chromatography–mass spectrometric method for the determination of short-chain fatty acids from intestinal fermentation. Food Chem. 2011;129:200–205. doi: 10.1016/j.foodchem.2011.04.022. [DOI] [Google Scholar]
  7. Brandle J, Domig KJ, Kneifel W. Relevance and analysis of butyric acid producing clostridia in milk and cheese. Food Control. 2016;67:96–113. doi: 10.1016/j.foodcont.2016.02.038. [DOI] [Google Scholar]
  8. Brigidi P, Vitali B, Swennen E, Bazzocchi G, Matteuzzi D. Effects of probiotic administration upon the composition and enzymatic activity of human fecal microbiota in patients with irritable bowel syndrome or functional diarrhea. Res Microbiol. 2001;152:735–741. doi: 10.1016/S0923-2508(01)01254-2. [DOI] [PubMed] [Google Scholar]
  9. Butel MJ. Probiotics, gut microbiota and health. Med Mal Infect. 2014;44:1–8. doi: 10.1016/j.medmal.2013.10.002. [DOI] [PubMed] [Google Scholar]
  10. Cabaton NJ, Canlet C, Wadia PR, Tremblay-Franco M, Gautier R, Molina J, Sonnenschein C, Cravedi JP, Rubin BS, Soto AM, Zalko D. Effects of low doses of bisphenol A on the metabolome of perinatally exposed CD-1 mice. Environ Health Perspect. 2013;121:586–593. doi: 10.1289/ehp.1205588. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Den Besten G, Van Eunen K, Groen AK, Venema K, Reijngoud DJ, Bakker BM. The role of short-chain fatty acids in the interplay between diet, gut microbiota, and host energy metabolism. J Lipid Res. 2013;54:2325–2340. doi: 10.1194/jlr.R036012. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Fuller R. Probiotics in man and animals. J Appl Bacteriol. 1989;66:365–378. doi: 10.1111/j.1365-2672.1989.tb05105.x. [DOI] [PubMed] [Google Scholar]
  13. George Kerry R, Patra JK, Gouda S, Park Y, Shin HS, Das G. Benefaction of probiotics for human health: A review. J Food Drug Anal. 2018;26:927–939. doi: 10.1016/j.jfda.2018.01.002. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Gibson GR, Beatty ER, Wang X, Cummings JH. Selective stimulation of bifidobacteria in the human colon by oligofructose and inulin. Gastroenterology. 1995;108:975–982. doi: 10.1016/0016-5085(95)90192-2. [DOI] [PubMed] [Google Scholar]
  15. Gibson GR, Probert HM, Loo JV, Rastall RA, Roberfroid MB. Dietary modulation of the human colonic microbiota: Updating the concept of prebiotics. Nutr Res Rev. 2004;17:259–275. doi: 10.1079/NRR200479. [DOI] [PubMed] [Google Scholar]
  16. Gibson GR, Roberfroid MB. Dietary modulation of the human colonic microbiota: Introducing the concept of prebiotics. J Nutr. 1995;125:1401–1412. doi: 10.1093/jn/125.6.1401. [DOI] [PubMed] [Google Scholar]
  17. Goldmann T, Perisset A, Scanlan F, Stadler RH. Rapid determination of furan in heated foodstuffs by isotope dilution solid phase micro-extraction-gas chromatography-mass spectrometry (SPME-GC-MS) Analyst. 2005;130:878–883. doi: 10.1039/b419270b. [DOI] [PubMed] [Google Scholar]
  18. Ham JS, Jeong SG, Noh YB, Shin JH, Han GS, Chae HS, Yoo YM, Ahn JN, Lee JW, Jo C, Lee YK. Effects of gamma irradiation on Queso Blanco cheese. Korean J Dairy Sci Technol. 2007;25:15–20. [Google Scholar]
  19. Hemalatha R, Ouwehand AC, Saarinen MT, Prasad UV, Swetha K, Bhaskar V. Effect of probiotic supplementation on total lactobacilli, bifidobacteria and short chain fatty acids in 2-5-year-old children. Microb Ecol Health Dis. 2017;28:1298340. doi: 10.1080/16512235.2017.1298340. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Hilton E, Kolakowski P, Singer C, Smith M. Efficacy of Lactobacillus GG as a diarrheal preventive in travelers. J Travel Med. 1997;4:41–43. doi: 10.1111/j.1708-8305.1997.tb00772.x. [DOI] [PubMed] [Google Scholar]
  21. Holzapfel WH, Haberer P, Snel J, Schillinger U, J Huis In't Veld JH. Overview of gut flora and probiotics. Int J Food Microbiol. 1998;41:85–101. doi: 10.1016/S0168-1605(98)00044-0. [DOI] [PubMed] [Google Scholar]
  22. Hooper LV, Wong MH, Thelin A, Hansson L, Falk PG, Gordon JI. Molecular analysis of commensal host-microbial relationships in the intestine. Science. 2001;291:881–884. doi: 10.1126/science.291.5505.881. [DOI] [PubMed] [Google Scholar]
  23. Kalivodova A, Hron K, Filzmoser P, Najdekr L, Janeckova H, Adam T. PLS-DA for compositional data with application to metabolomics. J Chemom. 2015;29:21–28. doi: 10.1002/cem.2657. [DOI] [Google Scholar]
  24. Lahtinen S, Ouwehand AC, Salminen S, Wright A. Lactic acid bacteria: Microbiological and functional aspects. 4th ed. CRC Press; Boca Raton, FL, USA: 2011. [Google Scholar]
  25. Lee WK, Lee SM, Bae HS, Baek YJ. Effect of Bifidobacterium longum HY8001 administration on human fecal bacterial enzymes and microflora. Korean J Appl Microbiol Biotechnol. 1999;27:267–272. [Google Scholar]
  26. Lee YK, Puong KY, Ouwehand AC, Salminen S. Displacement of bacterial pathogens from mucus and Caco-2 cell surface by lactobacilli. J Med Microbiol. 2003;52:925–930. doi: 10.1099/jmm.0.05009-0. [DOI] [PubMed] [Google Scholar]
  27. Lindon JC, Holmes E, Nicholson JK. Toxicological applications of magnetic resonance. Prog Nucl Magn Reson Spectrosc. 2004;45:109–143. doi: 10.1016/j.pnmrs.2004.05.001. [DOI] [Google Scholar]
  28. Marteau P, Seksik P, Jian R. Probiotics and intestinal health effects: A clinical perspective. Br J Nutr. 2002;88:S51–S57. doi: 10.1079/BJN2002629. [DOI] [PubMed] [Google Scholar]
  29. Martinez FA, Balciunas EM, Converti A, Cotter PD, De Souza Oliveira RP. Bacteriocin production by Bifidobacterium spp. A review. Biotechnol Adv. 2013;31:482–488. doi: 10.1016/j.biotechadv.2013.01.010. [DOI] [PubMed] [Google Scholar]
  30. Matsumoto M, Tadenuma T, Nakamura K, Kume H, Imai T, Kihara R, Watanabe M, Benno Y. Effect of Bifidobacterium lactis LKM 512 yoghurt on fecal microflora in middle to old aged persons. Microb Ecol Health Dis. 2000;12:77–80. doi: 10.1080/089106000435455. [DOI] [Google Scholar]
  31. Mitsuoka T, Morishita Y, Terada A, Yamamoto S. A simple method ("plate-in-bottle method") for the cultivation of fastidious anaerobes. Jpn J Microbiol. 1969;13:383–385. doi: 10.1111/j.1348-0421.1969.tb00482.x. [DOI] [PubMed] [Google Scholar]
  32. Nicholson JK, Holmes E, Kinross J, Burcelin R, Gibson G, Jia W, Pettersson S. Host-gut microbiota metabolic interactions. Science. 2012;336:1262–1267. doi: 10.1126/science.1223813. [DOI] [PubMed] [Google Scholar]
  33. O'mahony L, Mccarthy J, Kelly P, Hurley G, Luo F, Chen K, O'sullivan GC, Kiely B, Collins JK, Shanahan F, Quigley EM. Lactobacillus and Bifidobacterium in irritable bowel syndrome: Symptom responses and relationship to cytokine profiles. Gastroenterology. 2005;128:541–551. doi: 10.1053/j.gastro.2004.11.050. [DOI] [PubMed] [Google Scholar]
  34. Ong L, Henriksson A, Shah NP. Development of probiotic Cheddar cheese containing Lactobacillus acidophilus, Lb. casei, Lb. paracasei and Bifidobacterium spp. and the influence of these bacteria on proteolytic patterns and production of organic acid. Int Dairy J. 2006;16:446–456. doi: 10.1016/j.idairyj.2005.05.008. [DOI] [Google Scholar]
  35. Prasanna PHP, Grandison AS, Charalampopoulos D. Bifidobacteria in milk products: An overview of physiological and biochemical properties, exopolysaccharide production, selection criteria of milk products and health benefits. Food Res Int. 2014;55:247–262. [Google Scholar]
  36. Qin J, Li R, Raes J, Arumugam M, Burgdorf KS, Manichanh C, Nielsen T, Pons N, Levenez F, Yamada T, Mende DR, Li J, Xu J, Li S, Li D, Cao J, Wang B, Liang H, Zheng H, Xie Y, Tap J, Lepage P, Bertalan M, Batto JM, Hansen T, Le Paslier D, Linneberg A, Nielsen HB, Pelletier E, Renault P, Sicheritz-Ponten T, Turner K, Zhu H, Yu C, Li S, Jian M, Zhou Y, Li Y, Zhang X, Li S, Qin N, Yang H, Wang J, Brunak S, Dore J, Guarner F, Kristiansen K, Pedersen O, Parkhill J, Weissenbach J, Meta HITC, Bork P, Ehrlich SD, Wang J. A human gut microbial gene catalogue established by metagenomic sequencing. Nature. 2010;464:59–65. doi: 10.1038/nature08821. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Roberfroid M, Gibson GR, Hoyles L, McCartney AL, Rastall R, Rowland I, Wolvers D, Watzl B, Szajewska H, Stahl B, Guarner F, Respondek F, Whelan K, Coxam V, Davicco MJ, Leotoing L, Wittrant Y, Delzenne NM, Cani PD, Neyrinck AM, Meheust A. Prebiotic effects: Metabolic and health benefits. Br J Nutr 104 Suppl. 2010;2:S1–S63. doi: 10.1017/S0007114510003363. [DOI] [PubMed] [Google Scholar]
  38. Strompfova V, Pogany Simonova M, Gancarcikova S, Mudronova D, Farbakova J, Madari A, Laukova A. Effect of Bifidobacterium animalis B/12 administration in healthy dogs. Anaerobe. 2014;28:37–43. doi: 10.1016/j.anaerobe.2014.05.001. [DOI] [PubMed] [Google Scholar]
  39. Sullivan A, Nord CE. The place of probiotics in human intestinal infections. Int J Antimicrob Agents. 2002;20:313–319. doi: 10.1016/S0924-8579(02)00199-1. [DOI] [PubMed] [Google Scholar]
  40. Tannock GW. Molecular assessment of intestinal microflora. Am J Clin Nutr. 2001;73:410S–414S. doi: 10.1093/ajcn/73.2.410s. [DOI] [PubMed] [Google Scholar]
  41. Van Zanten GC, Knudsen A, Roytio H, Forssten S, Lawther M, Blennow A, Lahtinen SJ, Jakobsen M, Svensson B, Jespersen L. The effect of selected synbiotics on microbial composition and short-chain fatty acid production in a model system of the human colon. PLOS ONE. 2012;7:e47212. doi: 10.1371/journal.pone.0047212. [DOI] [PMC free article] [PubMed] [Google Scholar]
  42. Wang Y, Xu N, Xi A, Ahmed Z, Zhang B, Bai X. Effects of Lactobacillus plantarum MA2 isolated from Tibet kefir on lipid metabolism and intestinal microflora of rats fed on high-cholesterol diet. Appl Microbiol Biotechnol. 2009;84:341–347. doi: 10.1007/s00253-009-2012-x. [DOI] [PubMed] [Google Scholar]

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