Figure 4.

Distributions and mean intensities of histological markers in the prelimbic PFC following SS or 6 hr ASD conditions. (A) Within the prelimbic PFC, 8-oxo-dG, PV, and WFA intensities were significantly greater in ASD rats, compared with SS rats. (B) 6 hr ASD led to a significant change in 8-oxo-dG, PV, and WFA intensity distributions, compared with rats in the SS condition. (C) 8-oxo-dG intensity was significantly elevated following ASD treatment when identified to colocalize with PV or WFA staining in the prelimbic PFC. Prelimbic PFC PV intensity colocalizing with 8-oxo-dG was significantly higher in ASD-treated rats than in SS rats. Additionally, WFA intensities were elevated around 8-oxo-dG and PV neurons in rats experiencing ASD, compared with SS. (D) 6 hr ASD significantly altered 8-oxo-dG intensity distributions on cells colocalizing with PV and WFA, compared with SS. Similarly, ASD altered PV intensity distributions, compared with SS conditions, when colocalizing with 8-oxo-dG or WFA staining. Lastly, WFA labeled populations colocalizing with 8-oxo-dG or PV distributions in ASD rats significantly differed from rats in the SS condition. (E) Within the prelimbic PFC, 8-oxo-dG labeling that colocalized with both PV and WFA was significantly more intense in ASD-treated rats. PV staining that colocalized with both 8-oxo-dG and WFA was significantly more intense in ASD-treated rats. Similarly, 8-oxo-dG and PV neurons that were surrounded by WFA were significantly more intense in ASD-treated rats, compared with SS rats. (F) 6 hr ASD resulted in a significant shift in 8-oxo-dG intensity distributions on cells colocalizing with PV and WFA, compared with SS groups. ASD also shifted the PV intensity distribution, compared with SS conditions, when colocalizing with 8-oxo-dG and WFA labeling. Lastly, the distribution of WFA intensity in populations colocalizing with 8-oxo-dG and PV significantly differed between SS and ASD conditions. N = 4, *p < 0.05 for the difference between ASD and SS means.