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SHK protects APAP-induced hepatotoxicity via Akt/GSK3β/Nrf2 in mice. (A–C) Effects of SHK on phosphorylation of Akt and GSK3β, as well as Nrf2 protein expression analyzed by Western blotting. (D) LY294002 (40 mg/kg, i.p., 2 h before SHK administration), and then mice were administered SHK i.p. for 1 h. Then, the mice were injected with APAP for 9 h. Representative images of H&E staining of liver tissues.
