Table 2.
The individual polyphenols of the hydroalcoholic extract of the aerial part of Ephedra alata Decne. (EA), and the LC-MS parameters.
| Compounds | Retention Time Column I (min) | Retention Time Column II (min) | m/z Signal | EA in Hydroethanolic Extract | EA in Hydromethanolic Extract Chromatographic Column I | Calibration Curve | |
|---|---|---|---|---|---|---|---|
| Column I | Column II | ||||||
| µg/mL ± SD (µg/mg) | µg/mL ± SD (µg/mg) | (µg/mL ± SD) (µg/mg) | |||||
| Gallic acid | 4.826 | 4.745 | 169 | nd | nd | nd | y = 8.470 e-006x (r = 0.9996); |
| Protocatecuic acid | 11.982 | 11.774 | 153 | nd | nd | nd | y = 8.036 e-006x (r = 0.9990); |
| Caffeic acid | 18.605 | 18.396 | 179 | 0.709 ± 0.279 (0.014) | nd | 0.389 ± 0.49 (0.008) | y = 7.110 e-006x (r = 0.9990); |
| Epicatechin | 22.910 | 23.242 | 289 | nd | 7.736 ± 0.297 (0.155) | nd | y = 3.881 e-005x (r = 0.9996); |
| p-coumaric acid | 24.374 | 24.454 | 163 | 0.241 ± 0.028 (0.005) | nd | 0.261 ± 0.021 (0.005) | y = 1.1566 e-006x (r = 0.9997); |
| Ferulic acid | 26.333 | 24.883 | 193 | nd | nd | nd | y = 1.172 e-006x (r = 0.9999); |
| Rutin | 27.290 | 26.086 | 609 | nd | nd | nd | y = 1.813 e-005x (r = 0.9999) |
| Rosmarinic acid | 28.252 | 29.129 | 359 | 0.915 ± 0.008 (0.018) | 0.397 ± 0.002 (0.008) | 0.807 ± 0.049 (0.016) | y = 1.018 e-006x (r = 0.9982); |
| Resveratrol | 29.749 | 29.887 | 227 | 9.403 ± 0.008 (0.188) | 12.963 ± 0.454 (0.259) | 10.387 ± 0.422 (0.207) | y = 6.388 e-006x (r = 0.9945); |
| Quercitin | 32.591 | 31.841 | 301 | 2.873 ± 0.70 (0.057) | 2.387 ± 0.06 (0.048) | 4.572 ± 0.384 (0.091) | y = 1.001 e-005x (r = 0.9992); |
| Kaempferol | 34.470 | 34.378 | 285 | 24.297 ± 2.73 (0.485) | 6.814 ± 0.179 (0.136) | 28.675 ± 2.579 (2.867) | y = 3.273 e-005x (r = 0.9990). |
nd = not detected.