Table 1. Tm and ΔTm Values of RNA Duplexesa.
duplex | GC content (%) | Tm (°C) at pH 3.6 | Tm (°C) at pH 7.1 | ΔTm (°C) |
---|---|---|---|---|
1 | 53.8 | 35.5 ± 0.5 | 65.7 ± 0.3 | 30.2 |
2 | 30.8 | 17.0 ± 0.0 | 50.3 ± 0.3 | 33.3 |
3 | 69.2 | 45.8 ± 0.3 | 75.3 ± 0.3 | 29.5 |
4 | 50.0 | 56.5 ± 0.5 | 84.2 ± 0.3 | 27.7 |
Values determined by UV thermal melting experiments (±standard deviation). Conditions: 100 mM Na+, 10 mM citrate buffer, pH 3.6 or 7.1.