Figure 7.

Topological view of gene clouds that contain the gene names of differentially methylated CpG islands associated with a known gene due to ancestral exposure to the weakly estrogenic PCB, Aroclor A1221 (A1221) or the anti-androgenic fungicide, Vinclozolin (VIN) relative to control of F3 male rats. Each vertex of a triangle represents a gene cloud that contains all affected genes due to treatment in F3 sperm, central amygdala (CeA), and CA3 of the hippocampus (clockwise). Each edge of a triangle represents differentially methylated CpG islands identified as significantly altered by treatment in both tissues from which an edge originates. The gene cloud at the center of a triangle represents known genes that are affected in all three tissues due to ancestral exposure. CpG islands that were not associated with a known gene (i.e., located in intergenic space) have been removed from this analysis. Within sperm, more sites are affected in VIN, but both brain regions have more affected sites due to A1221 treatment. The number of sites evident in both sperm and a brain area was low and unaffected by treatment.