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. 2018 Dec 22;11(1):106–115. doi: 10.1080/19420862.2018.1525253

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© 2018 The Author(s). Published with license by Taylor & Francis Group, LCC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 4. — Mass spectrum, fragment ions, and sequence map of Fc/2-G0F glycoform fragmentation. A Mass spectrum of CAD fragmentation of Fc/2-G0F. Inset, representative fragment ions from labile glycan fragmentation. B Representative fragment ions from ECD and CAD fragmentations localizing the glycosylation site at Asn61. C Fc/2 subunit sequence map showing bond cleavages and glycosylation site. The glycosylation site at Asn61 is highlighted in red. The amino acid number here is based on the Fc/2 subunit sequence.