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. 2018 Dec 12;11(1):26–44. doi: 10.1080/19420862.2018.1550321

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© 2018 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 9. — Specificity analyses for library-derived and designer lead IgGs by flow cytometry using transiently-transfected HEK-293 cells.

Analyses of binding specificity were performed on HEK-293 cells transiently transfected with plasmids encoding either (A) human PD1 and human VEGFR2, (B) human FZD5 and human ULBP2. All plots show the target of interest transfected cells (grey line) versus ZS green marker-only transfected cells (black line). Each antibody was used in repeat staining at 5 μg/ml. These analyses confirmed that all antibodies (other than the isotype control IgG1) exhibited binding to PD1, but no antibody exhibited measurable signal on ZS-green, VEGFR2, FZD5 or ULBP2 transfected cells.