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. 2019 Jan 3;8:e39180. doi: 10.7554/eLife.39180

Key resources table.

Reagent type
(species) or resource		 Designation Source or reference Identifiers Additional
information
Gene (Rattus Norvegicus) pick1 UNIPROT - Q9EP80
Gene
(Rattus Norvegicus)		 gluA2 UNIPROT - P19491
Gene
(Rattus Norvegicus)		 B2AR UNIPROT - P10608
Gene
(Rattus Norvegicus)		 B1AR UNIPROT - P18090
Gene
(Rattus Norvegicus)		 PSD95 UNIPROT - P31016
Recombinant
DNA reagent		 FLAG-β2 DAT8 LKV Madsen et al., 2012 See Materials and
methods Section		 FLAG-β2ARHis6 Provided by Dr.
Mark von Zastrow
, vector: pcDNA3.1,
Recombinant DNA reagent FLAG-β2 DAT8 LKI This paper See Materials and
methods Section
Recombinant
DNA reagent		 FLAG-β2 DAT8 LKA This paper See Materials and
methods Section
Recombinant
DNA reagent		 FLAG-β2 DAT8 LKV + A Madsen et al., 2012 See Materials andmethods Section
Recombinant
DNA reagent		 FLAG-β1AR
Recombinant
DNA reagent		 SF-GluA2 This paper See Materials and
methods Section		 pRK5 pHluorin-GluA2 was kindly provided by
Dr Richard Huganir. Vector pRK5
Recombinant
DNA reagent		 SF-GluA2 + A This paper See Materials and methods Section Vector pRK5
Recombinant
DNA reagent		 Tac-YFP-GluA2 This paper See Materials and
methods Section		 pcDNA3.1
Recombinant
DNA reagent		 Tac-YFP-GluA2+A This paper See Materials and
methods Section		 pcDNA3.1
Recombinant
DNA reagent		 Tac-YFP-DAT This paper See Materials andmethods Section pcDNA3.1
Recombinant DNA reagent Tac-YFP-DAT+A This paper See Materials andmethods Section pcDNA3.1
Recombinant DNA reagent PICK1 WT Madsen et al., 2005 See Materials and
methods Section		 pET41a
Recombinant DNA reagent PICK1 A87L Madsen et al., 2005 See Materials andmethods Section pET41a
Recombinant
DNA reagent		 PICK1 L121E, V125E Herlo et al., 2018 See Materials and
methods Section		 pET41a
Recombinant DNA reagent SNAP-PICK1 This paper See Materials and
 methods Section		 pET41a
Recombinant DNA reagent SNAP-PICK1 A87L This paper See Materials and
methods Section		 pET41a
Recombinant
DNA reagent		 PSD95 PDZ 1–2 Provided by Dr. Kristian Strømgaard
Recombinant
DNA reagent		 GFP PICK1 WT Herlo et al., 2018 See Materials and
methods Section		 pcDNA5/FRT/TO
Recombinant
DNA reagent		 GFP PICK1 V121E,
L125E		 Herlo et al., 2018 See Materials and
methods Section		 pcDNA5/FRT/TO
Recombinant
DNA reagent		 pOG44 vector V600520
(ThermoFisher)
Cell line
(Human)		 HEK293 GripTite Available from
Thermofisher: A14150		 RRID:CVCL_U428 Provided by Dr
Jonathan Javitch.
Vector pRK5
Cell line
(Human)		 Flp-In T-REx
293 PICK1		 R78007 (ThermoFisher); Madsen et al., 2012 RRID:CVCL_U427
Cell line
(Human)		 Flp-In T-REx
293 PICK1		 R78007 (ThermoFisher); This paper
Antibody ANTI-FLAG M1 F3040 (Sigma) RRID:AB_439712 (1:1000)
Antibody horseradish peroxidase-conjugated goat anti-mouse IgG (ThermoFisher) RRID:AB_2535981 (1:500)
Peptide,
recombinant
protein		 OrG DAT C13 Synthesized
(TAG-Copenhagen)
Chemical
compound, drug		 alprenolol A0360000 (Sigma)
Chemical compound, drug isoproterenol 1351005 (Sigma)
Chemical compound, drug poly-L-ornithine hydrobromide P-8638 (Sigma)
Other Alexa Fluor C5 malemide 488/568 A10254, A20341 (ThermoFisher)
Other SNAP-surface
dye 568/647		 S9112S, S9137 (New England
Biolabs)
Other Alexa Fluor 488
(NHS)		 A20000 (ThermoFisher)
Other SuperSignal ELISA Femto Substrate 37075 (Thermo
Fisher)
Software, algorithm Graphpad Prism
Software, algorithm ImageJ
Software,
algorithm		 Copasi