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. 2019 Jan 11;8:e42274. doi: 10.7554/eLife.42274

Integrin alpha11 is an Osteolectin receptor and is required for the maintenance of adult skeletal bone mass

Bo Shen 1, Kristy Vardy 1, Payton Hughes 1, Alpaslan Tasdogan 1, Zhiyu Zhao 1, Rui Yue 2, Genevieve M Crane 1, Sean J Morrison 1,3,4,
Editors: Didier Y Stainier5, Clifford J Rosen6
PMCID: PMC6349404  PMID: 30632962

Abstract

We previously discovered a new osteogenic growth factor that is required to maintain adult skeletal bone mass, Osteolectin/Clec11a. Osteolectin acts on Leptin Receptor+ (LepR+) skeletal stem cells and other osteogenic progenitors in bone marrow to promote their differentiation into osteoblasts. Here we identify a receptor for Osteolectin, integrin α11, which is expressed by LepR+ cells and osteoblasts. α11β1 integrin binds Osteolectin with nanomolar affinity and is required for the osteogenic response to Osteolectin. Deletion of Itga11 (which encodes α11) from mouse and human bone marrow stromal cells impaired osteogenic differentiation and blocked their response to Osteolectin. Like Osteolectin deficient mice, Lepr-cre; Itga11fl/fl mice appeared grossly normal but exhibited reduced osteogenesis and accelerated bone loss during adulthood. Osteolectin binding to α11β1 promoted Wnt pathway activation, which was necessary for the osteogenic response to Osteolectin. This reveals a new mechanism for maintenance of adult bone mass: Wnt pathway activation by Osteolectin/α11β1 signaling.

Research organism: Mouse

eLife digest

Throughout our lives, our bones undergo constant remodeling. Cells called osteoclasts break down old bone and cells called osteoblasts lay down new. Normally, the two cell types work in balance but if the rate of breakdown outpaces new bone formation the skeleton can become weak. This weakness leads to a condition called osteoporosis, in which people suffer from fragile bones. Osteoporosis is hard to reverse, in part because our ability to encourage new bone to form is limited.

In 2016, researchers discovered a protein called osteolectin, which promotes new bone formation during adulthood by helping skeletal stem cells transform into bone cells. But so far, it has been unclear how osteolectin achieves this. To investigate this further, Shen et al. – including some researchers involved in the 2016 study – marked osteolectin with a molecular tag and tested what it bound on the surface of mouse and human bone marrow cells.

The experiments revealed that osteolectin binds to a specific receptor protein called α11 integrin, which can only be found on skeletal stem cells and the osteoblasts they give rise to. Once osteolectin binds to the receptor, it activates a signaling pathway that induces the stem cells to develop into osteoblasts. Mice that lacked either osteolectin or α11 integrin produced less bone and lost bone tissue faster as adults.

Osteolectin could potentially be useful in the treatment of osteoporosis or broken bones. Since only skeletal stem cells and osteoblasts cells produce α11 integrin, osteolectin would specifically target these cells without affecting cells that do not form bones. A next step will be to assess how well osteolectin compares to existing treatments for fragile bones.

Introduction

The maintenance of the adult skeleton requires the formation of new bone throughout life as a result of the differentiation of skeletal stem/progenitor cells into osteoblasts. Leptin Receptor+ (LepR+) bone marrow stromal cells are the major source of osteoblasts and adipocytes in adult mouse bone marrow (Zhou et al., 2014). These cells arise postnatally in the bone marrow, where they are initially rare and make little contribution to the skeleton during development, but expand to account for 0.3% of cells in adult bone marrow (Mizoguchi et al., 2014; Zhou et al., 2014). Nearly all fibroblast colony-forming cells (CFU-F) in adult mouse bone marrow arise from these LepR+ cells, and a subset of LepR+ cells form multilineage colonies containing osteoblasts, adipocytes, and chondrocytes, suggesting they are highly enriched for skeletal stem cells (Zhou et al., 2014). LepR+ cells are also a critical source of growth factors that maintain hematopoietic stem cells and other primitive hematopoietic progenitors in bone marrow (Ding and Morrison, 2013; Ding et al., 2012; Himburg et al., 2018; Oguro et al., 2013).

To identify new growth factors in the bone marrow, we performed RNA-seq analysis on LepR+ cells and looked for transcripts predicted to encode secreted proteins with sizes and structures similar to growth factors and whose function had not been studied in vivo. We discovered that Clec11a, a secreted glycoprotein of the C-type lectin domain superfamily (Bannwarth et al., 1999; Bannwarth et al., 1998), was preferentially expressed by LepR+ cells (Yue et al., 2016). Prior studies had observed Clec11a expression in bone marrow but inferred based on colony-forming assays in culture that it was a hematopoietic growth factor (Hiraoka et al., 1997; Hiraoka et al., 2001). We made germline knockout mice and found it is not required for normal hematopoiesis but that it is required for the maintenance of the adult skeleton (Yue et al., 2016). The mutant mice formed their skeleton normally during development and were otherwise grossly normal as adults but exhibited significantly reduced osteogenesis and bone volume beginning by 2 months of age (Yue et al., 2016). Recombinant protein promoted osteogenic differentiation by bone marrow stromal cells in vitro and in vivo (Yue et al., 2016). Based on these observations we proposed to call this new osteogenic growth factor, Osteolectin, so as to have a name related to its biological function. Osteolectin/Clec11a is expressed by a subset of LepR+ stromal cells in the bone marrow as well as by osteoblasts, osteocytes, and hypertrophic chondrocytes. The discovery of Osteolectin offers the opportunity to better understand the mechanisms that maintain the adult skeleton; however, the Osteolectin receptor and the signaling mechanisms by which it promotes osteogenesis are unknown.

Several families of growth factors, and the signaling pathways they activate, promote osteogenesis, including Bone Morphogenetic Proteins (BMPs), Fibroblast Growth Factors (FGFs), Hedgehog proteins, Insulin-Like Growth Factors (IGFs), Transforming Growth Factor-betas (TGF-βs), and Wnts (reviewed by Karsenty, 2003; Kronenberg, 2003; Wu et al., 2016). Bone marrow stromal cells regulate osteogenesis by skeletal stem/progenitor cells by secreting multiple members of these growth factor families (Chan et al., 2015). The Wnt signaling pathway is a particularly important regulator of osteogenesis, as GSK3 inhibition and β-catenin accumulation promote the differentiation of skeletal stem/progenitor cells into osteoblasts (Bennett et al., 2005; Dy et al., 2012; Hernandez et al., 2010; Krishnan et al., 2006; Kulkarni et al., 2006; Rodda and McMahon, 2006). Consistent with this, mutations that promote Wnt pathway activation increase bone mass in humans and in mice (Ai et al., 2005; Balemans et al., 2001; Boyden et al., 2002) while mutations that reduce Wnt pathway activation reduce bone mass in humans and in mice (Gong et al., 2001; Holmen et al., 2004; Kato et al., 2002).

The Wnt pathway can be activated by integrin signaling. There are 18 integrin α subunits and 8 β subunits, forming 24 different functional integrin heterodimer complexes (Humphries et al., 2006; Hynes, 1992). Integrin signaling promotes Wnt pathway activation through Integrin-Linked Kinase (ILK)-mediated phosphorylation of GSK3 and nuclear translocation of β-catenin (Burkhalter et al., 2011; Delcommenne et al., 1998; Novak et al., 1998; Rallis et al., 2010). Conditional deletion of Ilk or Ptk2 (which encodes Focal Adhesion Kinase, FAK) from osteoblast progenitors reduces osteogenesis and depletes trabecular bone in adult mice (Dejaeger et al., 2017; Sun et al., 2016), suggesting a role for integrins in adult osteogenesis. Conditional deletion of β1 integrin from chondrocytes or skeletal stem/progenitor cells impairs chondrocyte function and skeletal ossification during development (Aszodi et al., 2003; Raducanu et al., 2009; Shekaran et al., 2014). Activation of αvβ1 signaling by Osteopontin (Chen et al., 2014) or α5β1 signaling by Fibronectin (Hamidouche et al., 2009; Moursi et al., 1997) promotes the osteogenic differentiation of mesenchymal progenitors. Germline deletion of integrin α10 leads to defects in chondrocyte proliferation and growth plate function (Bengtsson et al., 2005) and germline deletion of integrin α11 leads to defects in tooth development (Popova et al., 2007). However, little is known about which integrins are required for adult osteogenesis in vivo.

Results

Integrin α11 is selectively expressed by LepR +cells and osteoblasts

The Osteolectin/Clec11a gene first appeared in bony fish and is conserved among bony vertebrates (Yue et al., 2016). Osteolectin contains a glutamic acid-rich sequence, an alpha-helical leucine zipper, and a C-type lectin domain (Figure 1A and B) (Bannwarth et al., 1999; Bannwarth et al., 1998). To generate hypotheses regarding potential Osteolectin receptors, we examined the amino acid sequence and found two integrin-binding motifs, RGD (Gardner and Hynes, 1985; Pierschbacher and Ruoslahti, 1984; Plow et al., 1985) and LDT (Fong et al., 1997; Viney et al., 1996) in human (Figure 1A) and mouse Osteolectin (Figure 1B). One or both of these motifs were conserved across Osteolectin sequences in all bony vertebrates (Figure 1C). This raised the possibility that the Osteolectin receptor might be an integrin.

Figure 1. Osteolectin contains conserved integrin binding motifs and binds with high affinity to integrin α11β1.

Figure 1.

(A, B) The human (A) and mouse (B) Osteolectin proteins contain RGD and LDT sequences. (C) Alignment of Osteolectin amino acid sequences shows that the RGD and LDT domains are evolutionarily conserved among bony vertebrates. (D, E) RNA-seq analysis of integrin α (D) and β (E) subunits in PDGFRα+CD45-Ter119-CD31- bone marrow stromal cells from enzymatically dissociated adult bone marrow (n = 2 independent samples). These cells are uniformly positive for LepR expression (Zhou et al., 2014). (F) RNA-seq analysis of Itga1, Itga6, Itga11, and Itgav in PDGFRα+CD45-Ter119-CD31- bone marrow stromal cells, VE-Cadherin+ bone marrow endothelial cells, and whole bone marrow cells (n = 2 independent samples per cell population). (G) Itga11 expression in cell populations from mouse bone marrow by qRT-PCR (n = 3 independent samples per cell population). The markers used for the isolation of each cell population are shown in Supplementary file 1. (H) In MC3T3-E1 preosteoblast cells expressing Flag-tagged Osteolectin, anti-Flag antibody co-immunoprecipitated endogenous integrin β1 and integrin α11 with Flag-tagged Osteolectin (results are representative of two independent experiments). (I) Recombinant human Osteolectin (rhOln) selectively bound to recombinant human integrin α11β1 and α10β1, but not to other integrins (n = 3 independent experiments). (J) Integrin α11β1 bound Osteolectin and recombinant human Pro-Collagen 1α (rhCol1A) with similar affinities, but not bovine serum albumin (BSA) (n = 3 independent experiments). (K) Osteolectin, but not Pro-Collagen 1α, promoted osteogenic differentiation by MC3T3-E1 cells and human bone marrow stromal cells (n = 3 independent experiments). (L) 200 nM RGDS peptide inhibited the binding of integrin α11β1 to recombinant human Osteolectin. (M) 100 μM RGDS peptide inhibited osteogenic differentiation by MC3T3-E1 cells and human bone marrow stromal cells in response to 30 ng/ml of recombinant human Osteolectin. All numerical data reflect mean ±standard deviation. Statistical significance was determined with one-way (G) or two-way ANOVAs with Dunnett’s multiple comparisons tests (K) or Tukey’s multiple comparisons tests (M).

Figure 1—source data 1. Data for Figure 1.
DOI: 10.7554/eLife.42274.004

Given that bone marrow stromal cells undergo osteogenesis in response to Osteolectin (Yue et al., 2016), we examined the expression of all α and β integrins in mouse bone marrow stromal cells by RNA-seq analysis. Among the genes that encode α integrins, Itga1 (encoding α1), Itga6 (encoding α6), Itga11 (encoding α11) and Itgav (encoding αv), were strongly expressed by bone marrow stromal cells (Figure 1D). Among the genes that encode β integrins, only Itgb1 (encoding β1) was strongly expressed (Figure 1E). Itga1, Itga6, and Itgav were strongly expressed by both LepR+ cells and endothelial cells, and and are widely expressed in non-osteogenic cells, where they have known ligands (Belkin et al., 1990; Defilippi et al., 1991; Lee et al., 2006; Mahabeleshwar et al., 2006; Yang et al., 2008), suggesting they are less likely to encode the Osteolectin receptor (Figure 1F). In contrast, Itga11 was expressed exclusively by LepR+ cells, not by endothelial cells or other bone marrow cells (Figure 1F). Quantitative reverse transcription PCR (qRT-PCR) analysis of sorted bone marrow cells (Supplementary file 1 shows the markers used to isolate these cells) showed that Itga11 was highly expressed by LepR+CD45-Ter119-CD31- stromal cells and Col2.3-GFP+CD45-Ter119-CD31- osteoblasts but not by any hematopoietic stem or progenitor population (Figure 1G). The expression patterns of Itga11 and Itgb1 were thus consistent with a potential role in osteogenesis.

Consistent with our results, integrin α11 is expressed by human bone marrow stromal cells in a way that correlates with osteogenic potential in culture (Kaltz et al., 2010); however, α11 is not known to regulate osteogenesis. Integrin α11 heterodimerizes with integrin β1 (Velling et al., 1999) and the only known ligand for α11β1 is collagen (Popova et al., 2004; Velling et al., 1999). Few cells express integrin α11, and it has been studied less than other integrins. Itga11 deficient mice are growth retarded and have smaller bones, but this was thought to reflect a defect in incisor development that leads to malnutrition (Popova et al., 2007).

Osteolectin binds to α11β1 with nanomolar affinity

To test whether α11β1 binds Osteolectin, we overexpressed Flag-tagged human Osteolectin in MC3T3-E1 pre-osteoblast cells and immunoprecipitated with anti-Flag beads. The anti-Flag beads pulled down the tagged Osteolectin along with endogenous integrin α11 and integrin β1 (Figure 1H). We then tested the affinity of recombinant human Osteolectin for multiple recombinant human integrin complexes by a microtiter well binding assay. Osteolectin selectively bound to integrin α11β1 and α10β1, but not to other integrins, including αVβ1, αVβ3, α4β1, α9β1, αIIbβ3 or αMβ2 (Figure 1I). Integrin α10 is the gene most closely related to α11. Integrin α10 is expressed by osteoblasts and chondrocytes (Bengtsson et al., 2005; Engel et al., 2013) but only at a low level by bone marrow stromal cells (Figure 1D). The dissociation constant (kd) of Osteolectin for α10β1 and α11β1 was 0.3 nM whereas the kd of Osteolectin for other integrins was >100 nM. The kd of human Pro-Collagen 1α for α11β1 was also high (0.1 nM; Figure 1J); however, in contrast to Osteolectin, addition of Pro-Collagen 1α to culture, either by adding it to the culture medium or using it to coat the plates, did not promote osteogenesis by MC3T3-E1 cells or two primary human bone marrow stromal cell lines (hBMSC#1 or hBMSC#2; Figure 1K). A peptide that inhibits the binding of integrins to RGD-containing ligands, RGDS (Arg-Gly-Asp-Ser) (Gardner and Hynes, 1985; Plow et al., 1985; Ruoslahti, 1996), inhibited the binding of integrin α11β1 to Osteolectin (Figure 1L) and the osteogenic response of MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells to Osteolectin in culture (Figure 1M).

Osteolectin promotes Wnt pathway activation

MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells secrete Osteolectin into the culture medium (Figure 2A), consistent with our observation that Osteolectin is synthesized by a subset of LepR+ bone marrow stromal cells (Yue et al., 2016). Deletion of Osteolectin from these cell lines reduced their osteogenic differentiation in osteogenic differentiation medium (Figure 2B and C), demonstrating that autocrine Osteolectin production is part of what drives osteogenesis by these cells in culture.

Figure 2. Osteolectin activates Wnt pathway signaling in skeletal stem/progenitor cells.

Figure 2.

(A) Western blot of cell culture supernatant from parental or Osteolectin deficient MC3T3-E1 cells and human bone marrow stromal cells (hBMSC#1 and hBMSC#2 cells) (this blot is representative of blots from three independent experiments). (B) Osteogenic differentiation in culture of parental or Osteolectin deficient MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells. Alizarin red staining was performed after 14 days (for MC3T3-E1 cells) or 21 days (for hBMSC cells) to quantify osteoblast differentiation and mineralization (n = 3 independent experiments). (C) qRT-PCR analysis of Dmp1 transcript levels in MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells after 14 to 21 days of osteogenic differentiation (n = 5 independent experiments). (D) MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells were stimulated with recombinant mouse Osteolectin (rOln) in osteogenic differentiation medium then lysed 0, 15, 30, or 60 min later and immunoblotted for phospho-PI3K, phospho-Akt, phospho-GSK3, β-catenin, total GSK3, and Actin (results are representative of 3 independent experiments). (E) MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells were transferred into osteogenic differentiation medium for 24 hr with PBS or recombinant mouse Osteolectin, lysed, and immunoblotted for phospho-GSK3, β-catenin, and Actin (this blot is representative of blots from three independent experiments). (F) qRT-PCR analysis of Wnt target gene transcript levels (Alkaline phosphatase, Axin2, Lef1, or Runx2) in MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells 24 hr after transfer into osteogenic differentiation medium, with PBS or recombinant mouse Osteolectin (n = 5 independent experiments). (G) Parental or Osteolectin deficient MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells after 24 hr in osteogenic differentiation medium were lysed and immunoblotted for phospho-GSK3, β-catenin, and Actin (this blot is representative of blots from three independent experiments). (H) qRT-PCR analysis of Wnt target gene transcript levels in parental or Osteolectin deficient MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells 24 hr after transfer into osteogenic differentiation medium (n = 5 independent experiments). All numerical data reflect mean ±standard deviation. The statistical significance of differences was determined with two-way ANOVAs with Sidak’s multiple comparisons tests.

Figure 2—source data 1. Data for Figure 2.
DOI: 10.7554/eLife.42274.006

To assess the signaling mechanisms by which Osteolectin promotes osteogenesis, we treated parental MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells with recombinant Osteolectin and assessed the levels of phosphorylated PI3-kinase, Akt, and GSK3. The most prominent change we observed was a dramatic increase of GSK3 phosphorylation at Ser21/9 within 30 to 60 min of Osteolectin treatment in all three cell lines (Figure 2D). Phosphorylation at Ser21/9 inhibits the GSK3-mediated degradation of β-catenin, increasing β-catenin levels and promoting the transcription of Wnt pathway target genes (Cross et al., 1995; Peifer et al., 1994; Yost et al., 1996). We did not observe an increase in β-catenin levels within 1 hr of Osteolectin treatment, but did detect increased β-catenin levels in all three cell lines within 24 hr of Osteolectin treatment (Figure 2E). The transcription of several Wnt target genes, including Axin2 (Jho et al., 2002; Lustig et al., 2002; Yan et al., 2001; Yan et al., 2009), Lef1 (Filali et al., 2002; Gaur et al., 2005; Hovanes et al., 2001), Runx2 (Dong et al., 2006; Gaur et al., 2005), and Alkaline phosphatase (Rawadi et al., 2003), were activated within 24 hr of Osteolectin treatment (Figure 2F). Osteolectin deficient MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells had lower levels of phospho-GSK3 and β-catenin as compared to parental cells (Figure 2G) as well as significantly lower levels of Wnt target genes (Figure 2H). These data demonstrate that Osteolectin promotes Wnt pathway activation in osteogenic cells.

Osteogenesis in response to Osteolectin requires β-catenin

To test whether Wnt pathway activation phenocopies the effects of Osteolectin, we evaluated the effects of AZD2858, which inhibits GSK3 function and promotes β-catenin accumulation (Berg et al., 2012). As expected (Gilmour et al., 2013; Marsell et al., 2012; Sisask et al., 2013), AZD2858 increased GSK3 phosphorylation and β-catenin levels (Figure 3A) as well as osteogenic differentiation (Figure 3B) by MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells. Osteolectin also increased GSK3 phosphorylation, β-catenin levels, and osteogenic differentiation by MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells (Figure 3A and B). However, when the two agents were added together, there was no further promotion of osteogenic differentiation beyond the effects of the individual agents (Figure 3B). These data suggest that Osteolectin and GSK3/β-catenin act in the same pathway to promote osteogenic differentiation by mesenchymal progenitors.

Figure 3. Osteogenic differentiation in response to Osteolectin requires β-catenin.

Figure 3.

(A and B) MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells were transferred into osteogenic differentiation medium with PBS or 30 ng/ml recombinant mouse Osteolectin, as well as DMSO or 200 nM of the GSK3 inhibitor AZD2858. (A) Cells were lysed 24 hr later and immunoblotted for phospho-GSK3, β-catenin, and Actin. (B) Alizarin red staining after 14 days (MC3T3-E1 cells) or 21 days (hBMSC cells) to quantify osteoblast differentiation and mineralization (n = 3 independent experiments). (C and D) MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells were transferred into osteogenic differentiation medium with PBS or 30 ng/ml Osteolectin, as well as DMSO or 200 nM of the β-catenin inhibitor IWR-1-endo. (C) Cells were lysed 24 hr later and immunoblotted for phospho-GSK3, β-catenin, and Actin. (D) Alizarin red staining after 14 days (MC3T3-E1 cells) or 21 days (hBMSC cells) to quantify osteoblast differentiation and mineralization (n = 3 independent experiments). All numerical data reflect mean ±standard deviation. The statistical significance of differences was determined with two-way ANOVAs with Tukey’s multiple comparisons tests.

Figure 3—source data 1. Data for Data for Figure 3.
DOI: 10.7554/eLife.42274.008

To test whether Osteolectin requires β-catenin to promote osteogenesis, we evaluated an inhibitor of Wnt pathway signaling, IWR-1-endo, which depletes β-catenin by stabilizing Axin2 in the β-catenin destruction complex (Chen et al., 2009). As expected, Osteolectin increased β-catenin levels and osteogenesis by MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells while IWR-1-endo reduced β-catenin levels and osteogenesis (Figure 3C and D). When added together, IWR-1-endo blocked the effect of Osteolectin on β-catenin levels and osteogenesis (Figure 3C and D). This suggests that Osteolectin requires β-catenin to induce osteogenesis by mesenchymal progenitors.

Wnt pathway activation and osteogenesis by Osteolectin require integrin α11

To test if Integrin α11 is required for the osteogenic response to Osteolectin, we used CRISPR/Cas9 to delete Itga11 from MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells. Deletion of Itga11 significantly reduced osteogenesis by each cell line in culture (Figure 4A and B). Addition of recombinant Osteolectin to culture significantly promoted osteogenesis by parental, but not Itga11 deficient, MC3T3-E1, hBMSC#1, and hBMSC#2 cells (Figure 4A and B). Integrin α11 is therefore required by mouse pre-osteoblast cells and human bone marrow stromal cells to undergo osteogenesis in response to Osteolectin.

Figure 4. Integrin α11 is required for osteogenic differentiation and Wnt pathway activation in response to Osteolectin but exogenous collagen does not activate Wnt pathway signaling.

Figure 4.

(A) Osteogenic differentiation in culture of parental or Itga11 deficient MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells with PBS or recombinant mouse Osteolectin (n = 3 independent experiments). (B) qRT-PCR analysis of Dmp1 transcript levels in MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells after 14 to 21 days of osteogenic differentiation (n = 5 independent experiments). (C) Parental or Itga11 deficient MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells were transferred into osteogenic differentiation medium with or without recombinant mouse Osteolectin then lysed 24 hr later and immunoblotted for phospho-GSK3, β-catenin, and Actin (this blot is representative of blots from three independent experiments). (D) qRT-PCR analysis of Wnt target gene transcript levels in parental or Itga11 deficient MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells 24 hr after transfer into osteogenic differentiation medium with PBS or Osteolectin (n = 5 independent experiments). (E) MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells were transferred into osteogenic differentiation medium with PBS or 30 ng/ml Osteolectin, or 30 ng/ml recombinant Pro-Collagen 1α, then lysed 24 hr later and immunoblotted for phospho-GSK3, β-catenin, and Actin (this blot is representative of blots from three independent experiments). (F) qRT-PCR analysis of Wnt target gene transcript levels in MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells 24 hr after transfer into osteogenic differentiation medium with PBS or 30 ng/ml Osteolectin, or 30 ng/ml Pro-Collagen 1α (n = 5 independent experiments). (G) Primary mouse bone marrow stromal cells were adherently cultured in osteogenic differentiation medium. PBS (control), recombinant human Pro-Collagen 1α, or recombinant human Osteolectin was added, then the cells were lysed 1 hr later and lysates were immunoblotted for phospho-FAK, phospho-GSK3, total FAK, and Actin (this blot is representative of blots from three independent experiments). (H) Primary mouse bone marrow stromal cells adherently cultured in osteogenic differentiation medium were treated with PBS (control), recombinant human Pro-Collagen 1α, or recombinant human Osteolectin then lysed 24 hr later and lysates were immunoblotted for phospho-FAK, phospho-GSK3, β-catenin, total FAK, and Actin (this blot is representative of blots from three independent experiments). (I) Primary mouse bone marrow stromal cells adherently cultured in osteogenic differentiation medium were treated with 30 ng/ml recombinant human Osteolectin or PBS (control) then lysed 2, 4, 6, 12, or 24 hr later. Nuclear and cytosolic/membrane-associated fractions were isolated from lysates by centrifugation then immunoblotted for β-catenin. As loading controls, Histone H3 was blotted in the nuclear fraction and Actin was blotted in the cytosolic/membrane-associated fraction (this blot is representative of blots from three independent experiments). (J) qRT-PCR analysis of Ctnnb1 transcript levels in cells from the experiment in panel (I) (n = 3 independent experiments). (K) Primary mouse bone marrow stromal cells from Lepr-Cre; Itga11fl/fl or littermate control mice were adherently cultured in osteogenic differentiation medium. PBS (control), recombinant human Pro-Collagen 1α, or recombinant human Osteolectin was added then the cells were lysed 1 hr later and lysates were immunoblotted for phospho-FAK and FAK (this blot is representative of blots from two independent experiments). All numerical data reflect mean ±standard deviation. Statistical significance was determined with two-way ANOVAs with Tukey’s multiple comparisons tests (A, B and D), Dunnett’s multiple comparisons tests (F), or Sidak’s multiple comparisons tests (J).

Figure 4—source data 1. Data for Figure 4.
DOI: 10.7554/eLife.42274.010

The Itga11 deficient MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells also exhibited lower levels of GSK3 phosphorylation and β-catenin as compared to parental control cells (Figure 4C). Addition of recombinant Osteolectin to culture increased the levels of phosphorylated GSK3 and β-catenin in parental control cells but not in Itga11 deficient cells (Figure 4C). We also observed significantly lower levels of Wnt target gene transcripts in the Itga11 deficient cells (Figure 4D). Addition of recombinant Osteolectin to culture significantly increased the levels of Wnt target gene transcripts in parental control cells but not in Itga11 deficient cells (Figure 4D). Integrin α11 is therefore required by mouse pre-osteoblast cells and human bone marrow stromal cells to activate Wnt pathway signaling in response to Osteolectin. Given that Collagen 1α bound to α11β1 (Figure 1J) but did not promote osteogenesis by MC3T3-E1 cells, hBMSC#1 cells, or hBMSC#2 cells (Figure 1K), we tested whether Collagen 1α promoted Wnt pathway activation in these cells. Addition of recombinant Osteolectin to culture increased levels of phosphorylated GSK3, β-catenin, and Wnt target gene transcripts in MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells (Figure 4E and F); however, addition of Pro-Collagen 1α to these cells did not seem to have any effect on the levels of phosphorylated GSK3, β-catenin, or Wnt target gene transcripts in these cells (Figure 4E and F).

We also added Osteolectin or Pro-Collagen 1α to freshly isolated mouse bone marrow stromal cells in culture. Both Pro-Collagen 1α and Osteolectin promoted Focal Adhesion Kinase (FAK) phosphorylation at Tyrosine 397 within one hour of addition to culture without affecting total FAK levels (Figure 4G), consistent with the activation of integrin signaling (Cooper et al., 2003). Although Pro-Collagen 1α had not detectably affected GSK3 phosphorylation in MC3T3-E1 cells, hBMSC#1 cells, or hBMSC#2 cells (Figure 4E), it did increase the levels of phosphorylated GSK3 in primary mouse bone marrow stromal cells, as did Osteolectin (Figure 4G and H). Osteolectin, but not Pro-Collagen 1α, promoted β-catenin accumulation within 24 hr of treatment (Figure 4H). To more precisely assess the time required for Osteolectin to promote β-catenin accumulation, we treated primary mouse bone marrow stromal cells with Osteolectin or phosphate-buffered saline (PBS) then assessed the levels of nuclear versus cytosolic/membrane associated β-catenin 2, 4, 6, 12, and 24 hr later. Osteolectin treatment did not significantly affect the levels of cytosolic/membrane associated β-catenin at any time point, but did increase nuclear β-catenin within 4 hr of treatment (Figure 4I). Osteolectin treatment did not significantly affect Ctnnb1 (which encodes β-catenin) transcript levels in these cells (Figure 4J), suggesting that the increase in nuclear β-catenin reflected an inhibition of proteasomal degradation. Together, these data suggest that Osteolectin promoted integrin α11 signaling, leading to Wnt pathway activation, accumulation of nuclear β-catenin, and increased transcription of Wnt target genes involved in osteogenesis.

Exogenous Pro-Collagen 1α also appeared to activate integrin signaling, at least in primary mouse bone marrow stromal cells (Figure 4G), but not accumulation of nuclear β-catenin (Figure 4H) or increased transcription of Wnt target genes (Figure 4F), offering a potential explanation for its failure to promote osteogenesis. Itga11-deficiency blocked the ability of Osteolectin to promote FAK phosphorylation in primary bone marrow stromal cells, but did not affect the ability of Pro-Collagen 1α to promote FAK phosphorylation (Figure 4K). This suggests that Osteolectin promotes integrin signaling in an α11-dependent manner but that Pro-Collagen 1α promotes integrin signaling in an α11-independent manner. This was expected as bone marrow stromal cells express multiple integrins that are capable of functioning as collagen receptors, including α1β1, α10β1 and αVβ3 (Figure 1D and E) (Davis, 1992; Gullberg and Lundgren-Akerlund, 2002).

Conditional deletion of integrin α11 from LepR+ cells reduces Osteogenesis in vivo

To test whether Integrin α11 is necessary for osteogenesis in vivo we generated mice bearing a floxed allele of Itga11 (Figure 5—figure supplement 1A–C), then conditionally deleted it from skeletal stem and progenitor cells in the bone marrow using Lepr-Cre. Only 5% of osteoblasts derive from LepR+ cells at two months of age but this number increases to approximately 50% by 10 months of age (Zhou et al., 2014). Lepr-Cre; Itga11fl/fl mice did not exhibit the defects in incisor development (data not shown) or the growth retardation observed in germline Itga11-/- mice (Popova et al., 2007). Lepr-Cre; Itga11fl/fl mice appeared grossly normal (Figure 5A), with body lengths (Figure 5B), body masses (Figure 5C), and femur lengths (Figure 5D) that did not significantly differ from sex-matched littermate controls. However, qRT-PCR analysis showed that LepR+ bone marrow cells from Lepr-Cre; Itga11fl/fl mice had an approximately 85% reduction in Itga11 transcript levels as compared to LepR+ cells from control mice (Figure 5—figure supplement 1D). Serum Osteolectin levels did not significantly differ between Lepr-Cre; Itga11fl/fl mice and littermate controls at 2 or 12 months of age but were modestly higher in Lepr-Cre; Itga11fl/fl mice than in controls at 6 months of age (Figure 5E). This demonstrates that Integrin α11 is not required for the synthesis or secretion of Osteolectin.

Figure 5. Conditional Itga11 deletion from LepR+cells accelerates the loss of trabecular bone during aging.

(A) Lepr-Cre; Itga11fl/fl mice were grossly normal and indistinguishable from littermate controls. (B – D) Body length (B), body mass (C) and femur length (D) did not significantly differ between Lepr-Cre; Itga11fl/fl mice and sex-matched littermate controls at 2, 6, or 12 months of age (n = 4–7 mice per genotype per sex per time point, from at least three independent experiments). (E) ELISA measurement of serum Osteolectin levels in Lepr-Cre; Itga11fl/fl mice and littermate controls at 2, 6, and 12 months of age (n = 8 mice per genotype per time point from four independent experiments). (F) Representative microCT images of trabecular bone in the distal femur metaphysis of male Lepr-Cre; Itga11fl/fl mice and littermate controls at 2, 6, and 12 months of age. (G–L) microCT analysis of trabecular bone volume/total volume (G), trabecular number (H), trabecular bone thickness (I), trabecular bone spacing (J), connectivity density (K), and bone mineral density (L) in the distal femur metaphysis of Lepr-Cre; Itga11fl/fl mice and sex-matched littermate controls at 2, 6, and 12 months of age (n = 4–7 mice per genotype per sex per time point from at least three independent experiments). (M) Trabecular bone mineral apposition rate based on calcein double labelling in the distal femur metaphysis (n = 3 mice per genotype per sex per time point). (N) Bone resorption rate analysis by measuring the deoxypyridinoline/creatinine ratio in the urine of LepR-Cre; Itga11fl/fl mice and littermate controls at 6 and 12 months of age (n = 4–5 mice per genotype per time point from three independent experiments). (O) Serum P1NP levels in LepR-Cre; Itga11fl/fl mice and sex-matched littermate controls at 6 and 12 months of age (n = 4 mice per genotype per time point from three independent experiments). All numerical data reflect mean ±standard deviation. The statistical significance of differences was determined with two-way ANOVAs with Sidak’s multiple comparisons tests.

Figure 5—source data 1. Data for Data for Figure 5.
DOI: 10.7554/eLife.42274.012

Figure 5.

Figure 5—figure supplement 1. Generation of an Itga11 floxed mice.

Figure 5—figure supplement 1.

(A) DNA surrounding Itga11 exon3 was used as homologous arms for recombineering. The recombineering plasmid was modified by inserting loxP sites flanking exon3. Deletion of exon three leads to a frame shift that would be expected to give a complete loss of α11 function (Popova et al., 2007). The loxP insertion sites were chosen so as not to disrupt sequences conserved among species. Cas9 mRNA, sgRNAs, and the recombineering plasmid were microinjected into C57BL/Ka zygotes. Correctly-targeted founder mice (F0) were identified by Southern blotting (B) using an internal (3’) probe and an external (5’) probe to ensure the recombineering vector was integrated in the correct genomic location, and not in other regions of the genome. Mice were backcrossed at least three times onto a C57BL/Ka background before analysis. (C) PCR genotyping demonstrated germline transmission of the Itga11flox allele. (D) qRT-PCR analysis of Itga11 transcript levels in LepR+ cells from the bone marrow of Lepr-Cre; Itga11fl/fl and sex-matched littermate controls (n = 4 mice per genotype per time point from two independent experiments). All numerical data reflect mean ±standard deviation.
Figure 5—figure supplement 1—source data 1. Data for Figure 5—figure supplement 1.
DOI: 10.7554/eLife.42274.014

To test whether deletion of Itga11 from LepR+ cells affected osteogenesis in vivo, we performed micro-CT analysis of the distal femur from 2, 6, and 12 month old Lepr-Cre; Itga11fl/fl mice and sex-matched littermates. Consistent with the observation that LepR+ cells contribute little to skeletal development prior to 2 months of age (Zhou et al., 2014), we observed no significant difference in trabecular bone parameters between Lepr-Cre; Itga11fl/fl mice and sex-matched littermates at 2 months of age (Figure 5F–L). However, LepR+ cells and Osteolectin are necessary for adult osteogenesis (Yue et al., 2016; Zhou et al., 2014). Consistent with this, 6 and 12-month-old male and female Lepr-Cre; Itga11fl/fl mice had significantly reduced trabecular bone volume as compared to sex-matched littermate controls (Figure 5F and G). At 6 and 12 months of age, male and female Lepr-Cre; Itga11fl/fl mice also tended to have lower trabecular number (Figure 5H) and trabecular thickness (Figure 5I) than sex matched littermate controls. Calcein double labelling showed that the mineral apposition rate was significantly reduced in trabecular bone from Lepr-Cre; Itga11fl/fl mice as compared to sex-matched littermates at 6 and 12 months of age (Figure 5M). Levels of Procollagen type 1 N-terminal Propeptide (P1NP), a marker of bone formation, were also significantly lower in the serum of Lepr-Cre; Itga11fl/fl mice as compared to littermate controls at 6 and 12 months of age (Figure 5O). Integrin α11 is, therefore, required by LepR+ cells and their progeny for normal rates of trabecular bone formation and maintenance of trabecular bone volume during adulthood, phenocopying the accelerated trabecular bone loss in adult Osteolectin deficient mice (Yue et al., 2016).

While Lepr-Cre; Itga11fl/fl mice had significantly reduced rates of bone formation as compared to sex-matched littermates (Figure 5M), they did not significantly differ in the urinary bone resorption marker deoxypyridinoline at 6 or 12 months of age (Figure 5N; this was not tested in 2-month-old mice because no difference in bone parameters was observed at that age). This suggests that, like Osteolectin, Integrin α11 promotes bone formation but does not regulate bone resorption (Yue et al., 2016).

Osteolectin deficiency has a milder effect on cortical bone as compared to trabecular bone, with no significant reduction in cortical bone until after 10 months of age (Yue et al., 2016). Consistent with this, femur cortical bone parameters did not significantly differ between Lepr-Cre; Itga11fl/fl mice and sex-matched littermates at 2 or 6 month of age (Figure 6A–F). However, cortical bone mineral density was significantly lower in male and female Lepr-Cre; Itga11fl/fl mice as compared to sex-matched littermates at 12 months of age (Figure 6F). Calcein double labelling revealed that the mineral apposition rate was significantly reduced in cortical bone from male and female Lepr-Cre; Itga11fl/fl mice as compared to sex-matched littermate controls at 6 and 12 months of age (Figure 6G). Deletion of Integrin α11 from LepR+ cells thus reduces the rate of cortical bone formation during adulthood, slowly leading to a thinning of cortical bone that became apparent in the femurs at 12 months of age, phenocopying the slow loss of cortical bone in adult Osteolectin deficient mice (Yue et al., 2016).

Figure 6. Conditional Itga11 deletion from LepR+cells reduces cortical bone formation in adult mice.

Figure 6.

(A) Representative microCT images of cortical bone in the mid-femur diaphysis of male Lepr-Cre; Itga11fl/fl mice and littermate controls at 2, 6, and 12 months of age. (B–F) microCT analysis of the total area (B), cortical area (C), cortical thickness (D), cortical area/total area (E), and cortical bone mineral density (F) in the mid-femur diaphysis of Lepr-Cre; Itga11fl/fl mice and sex-matched littermate controls at 2, 6, and 12 months of age (n = 4–7 mice per genotype per sex per time point from at least three independent experiments). (G) Cortical bone mineral apposition rate based on calcein double labelling in the mid-femur diaphysis (n = 3–4 mice per genotype per sex per time point from three independent experiments). All numerical data reflect mean ±standard deviation. The statistical significance of differences was determined with two-way ANOVAs with Sidak’s multiple comparisons tests.

Figure 6—source data 1. Data for Figure 6.
DOI: 10.7554/eLife.42274.016

Integrin α11 is required in bone marrow stromal cells to respond to Osteolectin

To test whether Integrin α11 is necessary for the maintenance or the proliferation of skeletal stem/progenitor cells in the bone marrow, we cultured at clonal density enzymatically dissociated femur bone marrow cells from Lepr-Cre; Itga11fl/fl and sex-matched littermate control mice at 2 and 6 months of age. We observed a slight, but statistically significant, reduction in the frequency of cells that formed CFU-F colonies in Lepr-Cre; Itga11fl/fl mice at 2 months of age, though no significant difference was apparent at 6 months of age (Figure 7A). We observed no significant difference in the number of cells per colony at either age (Figure 7B). Integrin α11 is therefore not required for the maintenance of CFU-F in vivo or for their proliferation in vitro.

Figure 7. Integrin α11 is required by bone marrow stromal cells to undergo osteogenesis in response to Osteolectin.

(A) CFU-F frequency and (B) cells per CFU-F colony formed by bone marrow cells from Lepr-Cre; Itga11fl/fl mice and littermate controls at 2 and 6 months of age (n = 6–8 mice per genotype per time point from at least three independent experiments). (C–E) Osteogenic (C); n = 7 mice per genotype, total, from seven independent experiments), adipogenic (D); n = 6 mice per genotype, total, from six independent experiments), and chondrogenic (E); n = 5 mice per genotype, total, from five independent experiments) differentiation of bone marrow stromal cells cultured from the femurs of Lepr-Cre; Itga11fl/fl mice and sex-matched littermate controls at 2 months of age. (F) Recombinant mouse Osteolectin promoted osteogenic differentiation in culture by femur bone marrow stromal cells from control but not Lepr-Cre; Itga11fl/fl mice (n = 3 mice per genotype, total, from three independent experiments). (G) qRT-PCR analysis of Dmp1 transcript levels in cells from panel (F). (H–N) Subcutaneous injection of recombinant mouse Osteolectin daily for 28 days (50 μg/kg body mass/day) significantly increased trabecular bone volume and number in female control mice but not female Lepr-Cre; Itga11fl/fl mice. (H) Representative microCT images of trabecular bone in the distal femur metaphysis. (I–N) microCT analysis of trabecular bone volume/total volume (I), trabecular number (J), trabecular thickness (K), trabecular spacing (L), connectivity density (M), and bone mineral density (N) in the distal femur metaphysis (n = 5 mice per treatment, total, from three independent experiments). (O) The GSK3 inhibitor, AZD2858, rescued the osteogenic differentiation in culture of bone marrow stromal cells from the femurs of Lepr-Cre; Itga11fl/fl mice (n = 6 independent experiments in which cells from one mouse of each genotype were cultured in each experiment). (P) Western blotting of cultured cell lysates showed that AZD2858 promoted GSK3 phosphorylation and increased β-catenin levels in bone marrow stromal cells from Lepr-Cre; Itga11fl/fl mice and littermate controls. All numerical data reflect mean ±standard deviation. The statistical significance of differences was determined with Wilcoxon’s test followed by Holm-Sidak multiple comparisons adjustment (A and B), one-way (F), (G,) and O) ANOVAs with Sidak’s multiple comparisons tests, by paired t-tests (C–E), or by one-way ANOVAs with Tukey’s multiple comparisons tests (I–N).

Figure 7—source data 1. Data for Figure 7.
DOI: 10.7554/eLife.42274.018

Figure 7.

Figure 7—figure supplement 1. Osteolectin promotes Wnt target gene transcription in mouse bone marrow stromal cells in an integrin α11-dependent manner.

Figure 7—figure supplement 1.

(A) Bone marrow stromal cells from Lepr-Cre; Itga11fl/fl mice and sex-matched littermates at 2 months of age were cultured in osteogenic differentiation medium supplemented with PBS (control) or 30 ng/ml Osteolectin and qRT-PCR analysis of Wnt target gene transcript levels was performed 24 hr later (n = 3 independent experiments). (B) Recombinant mouse Osteolectin subcutaneously injected daily for 28 days (50 μg/kg body mass/day) increased Wnt target gene transcript levels in LepR+ bone marrow cells from control, but not Lepr-Cre; Itga11fl/fl, mice (n = 4 mice per genotype per time point from two independent experiments). All numerical data reflect mean ±standard deviation. Statistical significance was assessed with two-way ANOVAs with Tukey’s multiple comparisons tests (A) or Dunnett’s multiple comparisons tests (B).
Figure 7—figure supplement 1—source data 1. Data for Figure 7—figure supplement 1.
DOI: 10.7554/eLife.42274.020

To test whether integrin α11 regulates the differentiation of bone marrow stromal cells, we cultured CFU-F from Lepr-Cre; Itga11fl/fl and littermate control mice at clonal density, then replated equal numbers of cells from Itga11 deficient and control colonies into osteogenic or adipogenic culture conditions (Figure 7C and D). We also centrifuged 2 × 105 CFU-F cells from Lepr-Cre; Itga11fl/fl and control colonies to form pellets and then cultured them in chondrogenic medium (Figure 7E). Consistent with the decreased osteogenesis from Itga11 deficient mesenchymal cell lines in culture (Figure 4A) and the reduced osteogenesis in Lepr-Cre; Itga11fl/fl mice in vivo (Figure 5 and Figure 6), bone marrow stromal cells from Lepr-Cre; Itga11fl/fl mice formed significantly less bone in culture as compared to control colonies (Figure 7C). This demonstrates that, like Osteolectin, Integrin α11 promotes osteogenesis by bone marrow stromal cells. We did not detect any difference between Lepr-Cre; Itga11fl/fl and control colonies in adipogenic or chondrogenic differentiation (Figure 7D and E). This is also consistent with the Osteolectin deficiency phenotype, which reduced osteogenesis in vitro and in vivo without having any detectable effect on adipogenesis or chondrogenesis (Yue et al., 2016).

To test if Integrin α11 is necessary for the osteogenic response of bone marrow stromal cells to Osteolectin, we cultured CFU-F from the bone marrow of 2-month-old Lepr-Cre; Itga11fl/fl mice and littermate controls then added osteogenic differentiation medium with or without recombinant mouse Osteolectin. Osteolectin significantly increased osteogenic differentiation by control colonies, but Lepr-Cre; Itga11fl/fl colonies underwent significantly less osteogenesis and did not respond to Osteolectin (Figure 7F and G). Osteolectin treatment also significantly increased the levels of the Wnt target gene transcripts Alp, Axin2, Lef1, and Runx2 in cells from control mice, but not Lepr-Cre; Itga11fl/fl mice (Figure 7—figure supplement 1A). Bone marrow stromal cells thus require Integrin α11 to undergo osteogenesis in response to Osteolectin.

To test if bone marrow stromal cells require Integrin α11 to undergo osteogenesis in response to Osteolectin in vivo, we administered daily subcutaneous injections of recombinant mouse Osteolectin to 2-month-old Lepr-Cre; Itga11fl/fl and littermate control mice for 28 days. Osteolectin is functionally important for bone maintenance by 2 months of age given that Osteolectin deficient mice exhibit a significant reduction in trabecular bone volume at 2 months of age (Yue et al., 2016). Consistent with our prior study (Yue et al., 2016), in the distal femur metaphysis of control mice, Osteolectin treatment significantly increased trabecular bone volume (Figure 7H and I), trabecular bone number (Figure 7J), and trabecular connectivity density (Figure 7M), while significantly reducing trabecular spacing (Figure 7L). However, Osteolectin treatment had no significant effect on these parameters in Lepr-Cre; Itga11fl/fl mice (Figure 7H–N). Osteolectin treatment also significantly increased the levels of the Wnt target gene transcripts Alp, Lef1, and Runx2 in LepR+ cells isolated from the bone marrow of control mice, but not Lepr-Cre; Itga11fl/fl mice (Figure 7—figure supplement 1B). LepR+ bone marrow stromal cells and their progeny thus require Integrin α11 to undergo osteogenesis in response to Osteolectin in vivo. Neither Osteolectin administration nor Itga11 deficiency had any significant effect on cortical bone parameters in this relatively short-term experiment performed in young mice (data not shown).

To test if bone marrow stromal cells from Lepr-Cre; Itga11fl/fl mice retained the capacity to undergo osteogenesis upon Wnt pathway activation, we cultured these cells from 2-month-old Lepr-Cre; Itga11fl/fl and littermate control mice and treated half of the cultures with the Wnt pathway agonist, AZD2858. In control cultures, bone marrow stromal cells from Lepr-Cre; Itga11fl/fl mice underwent significantly less osteogenesis as compared to stromal cells from control mice (Figure 7O). Addition of AZD2858 significantly increased osteogenic differentiation from both Lepr-Cre; Itga11fl/fl and control stromal cells. In cultures treated with DMSO control, Lepr-Cre; Itga11fl/fl stromal cells had lower levels of phosphorylated GSK3 and β-catenin as compared to control stromal cells (Figure 7P). AZD2858 increased the levels of phosphorylated GSK3 and β-catenin in both Lepr-Cre; Itga11fl/fl and control stromal cells (Figure 7P). Lepr-Cre; Itga11fl/fl stromal cells thus retain the ability to undergo osteogenesis in response to Wnt pathway activation, even though they do not respond to Osteolectin.

Discussion

Our data demonstrate that integrin α11 is a physiologically important receptor for Osteolectin, mediating its effect on osteogenesis. Integrin α11 is expressed by LepR+ skeletal stem cells and osteoblasts but shows little expression in non-osteogenic cells (Figure 1G). Osteolectin bound selectively to α11β1 integrin, with nanomolar affinity (Figure 1I and J), and promoted Wnt pathway activation in bone marrow stromal cells (Figure 2). Integrin α11 was required in bone marrow stromal cells for Wnt pathway activation and osteogenesis in response to Osteolectin (Figure 4A–D). Blocking Wnt pathway activation in bone marrow stromal cells blocked the osteogenic effect of Osteolectin (Figure 3C–D). Conditional deletion of Itga11 from LepR+ cells phenocopied the effect of Osteolectin deficiency (Yue et al., 2016): in both cases the mice were grossly normal but exhibited accelerated bone loss during adulthood, particularly in trabecular bone (Figure 5). Like Osteolectin deficiency (Yue et al., 2016), Itga11 deficiency significantly reduced the rate of bone formation in adult mice (Figure 5M and O) without affecting the rate of bone resorption (Figure 5N). Bone marrow stromal cells from Lepr-Cre; Itga11fl/fl mice differentiated normally to adipocytes and chondrocytes (Figure 7A–E) but exhibited reduced osteogenic differentiation and did not respond to Osteolectin in vitro (Figure 7C, F and G and Figure 7—figure supplement 1A) or in vivo (Figure 7H–N and Figure 7—figure supplement 1B). Nonetheless, bone marrow stromal cells from Lepr-Cre; Itga11fl/fl mice retained the ability to form bone in response to a chemical inhibitor of GSK3, which activates the Wnt pathway (Figure 7O and P). We conclude that integrin α11 is required by skeletal stem/progenitor cells to undergo osteogenesis in response to Osteolectin.

Multiple factors promote osteogenesis by activating the Wnt pathway, including Wnts (Boyden et al., 2002; Cui et al., 2011; Gong et al., 2001), BMPs (Chen et al., 2007; Rawadi et al., 2003), hedgehog proteins (Mak et al., 2006), and parathyroid hormone (Bonnet et al., 2012; Kulkarni et al., 2005; Wan et al., 2008). Our data suggest that Osteolectin contributes to Wnt pathway activation in osteogenic stem/progenitor cells along with other factors.

While deficiency for integrin α11 phenocopied the effects of Osteolectin deficiency, we do not rule out a potential role for α10 integrin in mediating certain effects of Osteolectin. α10β1 also bound Osteolectin with nanomolar affinity (Figure 1I). Integrin α11 is more highly expressed than α10 by LepR+ cells (Figure 1D); however, integrin α10 is expressed by chondrocytes (Bengtsson et al., 2005; Reinisch et al., 2015). While we did not observe any cartilage defects in Osteolectin deficient mice (Yue et al., 2016), Osteolectin may promote the differentiation of hypertrophic chondrocytes into bone in adult mice, such as during fracture healing. Therefore, α10 integrin may mediate the effects of Osteolectin on hypertrophic chondrocytes while integrin α11 may mediate the effects of Osteolectin on skeletal stem/progenitor cells. It also remains possible that osteolectin has other receptors.

Osteolectin may not be the only osteogenic ligand for integrin α11. Collagen is a known ligand for α11β1 integrin (Popova et al., 2007). We found that collagen binds to α11β1 with nanomolar affinity (Figure 1J) and actives integrin signaling (Figure 4G and 4H, but we did not detect any effect of exogenous collagen on β-catenin accumulation (Figure 4F) or osteogenic differentiation (Figure 1K). This suggests that collagen may bind α11β1 in a way that regulates cell adhesion and migration but not osteogenic differentiation, at least in skeletal stem/progenitor cells. Alternatively, endogenous collagen may bind α11β1 differently than exogenous collagen, potentially promoting osteogenesis. Since Lepr-Cre; Itga11fl/fl mice delete Itga11 in postnatal bone marrow cells that exhibit little contribution to the skeleton prior to two months of age (Zhou et al., 2014), it remains untested whether integrin α11 regulates osteogenesis during fetal or early postnatal development. If so, this would raise the possibility of a distinct osteogenic ligand for α11 during development as Osteolectin deficient mice do not appear to exhibit defects in skeletal development (Yue et al., 2016).

While integrin α11 is not widely expressed by non-osteogenic cells, integrin α11 may have non-osteogenic functions in certain other cell types, or during development, in cells that are not competent to undergo osteogenesis. Integrin α11 is expressed by periodontal ligament fibroblasts and is required for the migration of these cells during ligament development, leading to a failure of tooth eruption in germline Itga11 deficient mice (Popova et al., 2007). This raises the possibility that collagen binding to α11β1 may have biologically distinct consequences in cells that are not competent to form bone.

Given that integrins can function as mechanosensors (Schwartz, 2010), our data raise the possibility that integrin α11 mediates the osteogenic response to mechanical loading in bones. Interestingly, it was recently discovered that skeletal stem cells in the developing jaw undergo osteogenesis in response to mechanical forces by activating FAK, suggesting the involvement of integrins in this process (Ransom et al., 2018).

In conclusion, we identify integrin α11 as an Osteolectin receptor and a new regulator of osteogenesis and adult skeleton maintenance. The identification of a new ligand/receptor pair that regulates the maintenance of the adult skeleton offers the opportunity to better understand the physiological and pathological mechanisms that influence skeletal homeostasis.

Materials and methods

Key resources table.

Reagent type
(species) or
resource
Designation Source or
reference
Identifiers Additional
information
Genetic reagent(M.musculus) Osteolectin-/- PMID: 27976999
Genetic reagent (M.musculus) Lepr-Cre PMID: 11283374 JAX Stock (008320)
Antibody rabbit polyclonal anti-phospho-PI3 Kinase Cell Signaling 4228S (1:1000)
Antibody rabbit polyclonal anti-phospho-Akt (Ser473) Cell Signaling 4060S (1:1000)
Antibody rabbit polyclonal anti-phospho-GSK-3α/β Cell Signaling 9331S (1:1000)
Antibody rabbit polyclonal anti-β-Catenin Cell Signaling 8480S (1:1000)
Antibody rabbit polyclonal anti-anti-β-Actin Cell Signaling 8457S (1:10000)
Antibody rabbit polyclonal anti-GSK-3b Cell Signaling 9315S (1:1000)
Antibody goat polyclonal anti-rabbit IgG, HRP-linked antibody Cell Signaling 7074S (1:5000)
Antibody horse polyclonal anti-mouse IgG, HRP-linked antibody Cell Signaling 7076S (1:5000)
Antibody donkey polyclonal anti-sheep IgG, HRP-linked antibody R and D Systems HAF016 (1:5000)
Antibody rabbit polyclonal anti-phospho-FAK (Y397) Cell Signaling 3283S (1:1000)
Antibody rabbit polyclonal anti-Histone H3 Cell Signaling 4499S (1:5000)
Antibody goat polyclonal anti-mouse Osteolectin R and D Systems AF3729 (1:1000)
Antibody sheep polyclonal anti-human Osteolectin R and D Systems AF1904 (1:1000)
Antibody rabbit polyclonal anti-Adiponectin Abcam ab181699 (1:1000)
Antibody rabbit polyclonal anti-Integrin α11 Abcam ab198826 (1:1000)
Antibody rabbit polyclonal anti-FAK Cell Signaling 3285P (1:1000)
Antibody mouse monoclonal anti-6x-His Tag antibody Thermo Fisher Scientific MA1-135 (1:1000)
Antibody rat polyclonal anti-CD45-APC Tonbo 20–0451 (1:200)
Antibody rat polyclonal anti-Ter119-APC Tonbo 20–5921 (1:200)
Antibody rat polyclonal anti-CD31-APC Biolegend 102410 (1:200)
Antibody goat polyclonal anti-Mouse Leptin R, Biotin R and D Systems AF497 (1:200)
Antibody mouse monoclonal anti-Streptavidin PE Biolegend 410504 (1:500)
Peptide, recombinant protein Bovine serum albumin Sigma-Aldrich A3156
Peptide, recombinant protein recombinant human pro-Collagen I α1 R and D Systems 6220 CL
Peptide, recombinant protein recombinant human osteolectin PMID: 27976999
Peptide, recombinant protein recombinant mouse osteolectin PMID: 27976999
Peptide, recombinant protein recombiant Integrin α11β1 protein R and D Systems 6357-AB
Peptide, recombinant protein recombiant Integrin α10β1 protein R and D Systems 5895-AB
Peptide, recombinant protein recombiant Integrin αVβ3 protein R and D Systems 3050-AV
Peptide, recombinant protein recombiant Integrin αVβ1 Protein R and D Systems 6579-AV
Peptide, recombinant protein recombiant Integrin α4β1 protein R and D Systems 5668-A4
Peptide, recombinant protein recombiant Integrin α9β1 protein R and D Systems 5438-A9
Peptide, recombinant protein recombiant Integrin αIIbβ3 Protein R and D Systems 7148-A2
Peptide, recombinant protein recombiant Integrin αMβ2 Protein R and D Systems 4047-AM
Chemical compound,drug DAPI Life Technologies D1306
Chemical compound, drug TRIzol LS Reagent Invitrogen 10296028
Chemical cmpound,drug Collagenase, Type 1 Worthington LS004196
Chemical compound, drug Dispase II Roche Diagnostic D4693
Chemical compound, drug DNase I Sigma-Aldrich 10 104 159 001
Chemical compound, drug IWR-1-endo Sigma-Aldrich 681669
Chemical compound, drug AZD2858 Selleck S7253
Chemical compound, drug Y-27632 Rock inhibitor Selleck S1049
Chemical compound, drug 4% paraformaldehyde in PBS Thermo Fisher Scientific J19943-K2
Cell lines (M.musculus) MC3T3-E1, subclone 4 ATCC CRL-2593
Cell lines (H.sapiens) hBMSC#1 ATCC PCS-500–012
Cell lines (H.sapiens) hBMSC#2 Lonza PT-2501
Commercial assay or kit Cell Culture Contamination Detection Kit Molecular Probes C-7028
Commercial assay or kit TMB stop solution KPL 95059–198
Commercial assay or kit SureBlue TMB Microwell Peroxidase Substrate KPL 95059–282
Commercial assay or kit HBSS, without Calcium and Magnesium Corning 21022CV
Commercial assay or kit HBSS, with Calcium and Magnesium Corning 21023CV
Commercial assay or kit CleanCap Cas9 mRNA TriLink L-7206
Commercial assay or kit MEGAshortscript T7 Transcription Kit Ambion AM1354
Commercial assay or kit MEGAclear Transcription Clean-Up Kit Ambion AM1908
Commercial assay or kit EDTA-free Protease Inhibitor Cocktail Sigma-Aldrich 11 836 170 001
Commercial assay or kit ECL Western Blotting Substrate Pierce 32106
Commercial assay or kit SuperScript III Reverse Transcriptase Invitrogen 18080044
Commercial assay or kit StemPro Osteogenesis Differentiation Kit Gibco A1007201
Commercial assay or kit StemPro Adipogenesis Differentiation Kit Gibco A1007001
Commercial assay or kit StemPro Chondrogenesis Differentiation Kit Gibco A1007101
Commercial assay or kit MicroVue DPD ELISA Kit Quidel 8007
Commercial assay or kit MicroVue Creatinine Assay Kit Quidel 8009
Commercial assay or kit Rat/Mouse PINP ELISA kit Immunodiagnostic Systems AC-33F1
Commercial assay or kit Nuclear/Cytosol Fractionation Kit Biovision K266
Other Fetal bovine serum Sigma-Aldrich F2442
Other Penicillin Streptomycin 100x solution HyClone SV3001
Other DMEM, low glucose Gibco 11885084
Other DMEM, high glucose Gibco D5671

Mice and cell lines

Lepr-cre mice were described previously (DeFalco et al., 2001) and obtained from the Jackson Laboratory (Stock No: 008320). Lepr-cre mice were backcrossed at least eight times onto a C57BL/Ka background. To generate Itga11fl/fl mice, CleanCap Cas9 mRNA (TriLink) and sgRNAs (transcribed using MEGAshortscript Kit (Ambion), purified using the MEGAclear Kit (Ambion)), and recombineering plasmids were microinjected into C57BL/Ka zygotes. Chimeric mice were genotyped by restriction fragment length poly-morphism (RFLP) analysis and confirmed by Southern blotting and sequencing of the targeted allele. Founders were mated with C57BL/Ka mice to obtain germline transmission then backcrossed with wild-type C57BL/Ka mice for at least three generations prior to analysis. This study was performed in accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. All procedures were approved by the UTSW Institutional Animal Care and Use Committee (protocol number 2016–101334 G).

Cell lines used in this study included mouse preosteoblast MC3T3-E1 cells (Subclone 4, ATCC CRL-2593), human bone marrow stromal cells from ATCC (PCS-500–012; referred to as hBMSC#1), and human bone marrow stromal cells from Lonza (PT-2501, hBMSC#2). The identity of MC3T3-E1 cells has been authenticated by ATCC, based on their expression of osteoblast marker genes including Bsp, Ocn, Pth and Pthrp. The identity of hBMSC#1 cells has been authenticated by ATCC using cell surface markes for these cells, including CD105, CD73, and CD44. The identity of hBMSC#2 cells has been authenticated by Lonza using cell surface markes for these cells, including CD105, CD166, CD73, and CD44. We found no contamination of these cells from yeast, fungi, gram-positive or gram-negative bateria using the Cell Culture Contamination Detection Kit (Molecular Probes). MC3T3-E1 cells were cultured in Alpha Minimum Essential Medium with ribonucleosides, deoxyribonucleosides, 2 mM L-glutamine and 1 mM sodium pyruvate, but without ascorbic acid (GIBCO, A1049001), and supplemented with 10% fetal bovine serum (Sigma, F2442) and penicillin-streptomycin (HyClone). MC3T3-E1 cells were used for experiments before passage 20. hBMSC cells were cultured in low glucose DMEM (Gibco) supplemented with 20% fetal bovine serum (Sigma, F2442) and penicillin-streptomycin (HyClone), and were used for experiments before passage 16.

Recombinant protein production and use

As described previously (Yue et al., 2016), mouse and human Osteolectin cDNA were cloned into pcDNA3 vector (Invitrogen) containing a C-terminal 1XFlag-tag and transfected into HEK293 cells with Akashi et al. (2000) (Invitrogen). Stably expressing cell lines were selected using 1 mg/ml G418 (Sigma) then cultured in DMEM plus 10% FBS (Sigma), and 1% penicillin/streptomycin (Invitrogen). Culture medium was collected every two days, centrifuged to eliminate cellular debris, and stored with 1 mM phenylmethylsulfonyl fluoride (Sigma) at 4°C to inhibit protease activity. One liter of culture medium was filtered through a 0.2 µm membrane (Nalgene) to eliminate cellular debris before being loaded onto a chromatography column containing 2 ml Anti-FLAG M2 Affinity Gel (Sigma), with a flow rate of 1 ml/min. The column was sequentially washed using 20 ml of high salt buffer (20 mM Tris-HCl, 300 mM KCl, 10% Glycerol, 0.2 mM EDTA) followed by 20 ml of low salt buffer (20 mM Tris-HCl, 150 mM KCl, 10% Glycerol, 0.2 mM EDTA) and finally 20 ml of PBS. The FLAG-tagged Osteolectin was then eluted from the column using 10 ml 3X FLAG peptide (100 mg/ml) in PBS. Eluted protein was concentrated using Amicon Ultra-15 Centrifugal Filter Units (Ultracel-10K, Millipore), then quantitated by SDS-PAGE and colloidal blue staining (Invitrogen) and stored at −80°C.

Recobinant human Pro-Collagen 1α was purchased from R and D Systems, and we removed the His tag using TEV protease (Sigma). After cleavage, we purified the untagged Pro-Collagen 1α using Ni-NTA agarose columns (Thermo Fisher Scientific) to separate it from the cleaved His tag, the His-tagged Pro-Collagen 1α, and the His-tagged TEV protease.

To add recombinant proteins in culture, recombinant human or mouse Osteolectin or Pro-Collagen 1α was added to osteogenic differentiation medium (described below). Unless otherwise specified, we used 30 ng/ml recombinant Osteolectin for in vitro assays. For in vivo use, recombinant mouse Osteolectin (50 μg/kg of body mass) was subcutaneously injected daily into 2-month-old female Lepr-Cre; Itga11fl/fl or littermate Itga11fl/fl control mice for 28 days. Mice receiving control injections received an equal volume of PBS.

Western blots and co-immunoprecipitation

Cells were cultured until confluent, then transferred into osteogenic differentiation medium with or without Osteolectin or small molecule inhibitors of Wnt pathway components. Prior to extracting proteins, cells were washed with PBS and then lysis buffer was added containing 50 mM Tris-HCl, 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM sodium vanadate, 0.5 mM sodium fluoride, and cOmplete Mini EDTA-free Protease Inhibitor Cocktail (Sigma). The cells were scraped off the plate in the lysis buffer, transferred to an Eppendorf tube on ice, incubated for 20 min with occasional vortexing, then centrifuged at 17,000xg for 10 min at 4°C to clear cellular debris. The cell lysates were Western blotted with the indicated antibodies and immunoreactive bands were detected using ECL reagent (Pierce). For some experiments, a Nuclear/Cytosol Fractionation Kit (Biovision) was used to separate the nuclear and cytosolic/membrane fractions of cell lysates. Antibodies used in this study include anti-Phospho-PI3 Kinase p85(Tyr458)/p55(Tyr199), anti-Phospho-Akt (Ser473), anti-Phospho-GSK-3α/β (Ser21/9), anti-Phospho-FAK (Y397), anti-β-Catenin, anti-β-Actin, anti-GSK-3β (27C10), anti-Histone H3, and anti-rabbit IgG, HRP-linked antibody from Cell Signaling, anti-mouse Osteolectin (AF3729) and anti-human Osteolectin (AF1904) antibodies from R and D Systems, anti-Adiponectin (ab181699) and anti-Integrin α11 antibody (ab198826) from Abcam.

For co-immunoprecipitation experiments, human Osteolectin cDNA was cloned into pcDNA3 vector (Invitrogen) containing a C-terminal 1XFlag-tag, then transfected into MC3T3-E1 cells with Morrison et al., 2000 (Invitrogen). After 48 hr, cells were solubilized in lysis buffer and cellular debris was cleared by centrifugation as described above, then lysates were immunoprecipitated with anti-FLAG M2 Affinity Gel (Sigma). After incubation of lysates with M2 Affinity Gel for 2 hr at 4°C, the gel was centrifuged and washed six times with lysis buffer. Immunoprecipitates were analyzed by western blotting.

qRT-PCR

For quantitative reverse transcription PCR (qRT-PCR), cells were lysed using TRIzol LS (Invitrogen). RNA was extracted and reverse transcribed into cDNA using SuperScript III (Invitrogen). qRT-PCR was performed using a Roche LightCycler 480. The primers used for qRT-PCR analysis of mouse RNA include: Osteolectin: 5’-AGG TCC TGG GAG GGA GTG-3’ and 5’-GGG CCT CCT GGA GAT TCT T-3’; Actb: 5’-GCT CTT TTC CAG CCT TCC TT-3’ and 5’-CTT CTG CAT CCT GTC AGC AA-3’; Lef1: 5’-TGT TTA TCC CAT CAC GGG TGG-3’ and 5’-CAT GGA AGT GTC GCC TGA CAG-3’; Runx2: 5’-TTA CCT ACA CCC CGC CAG TC-3’ and 5’-TGC TGG TCT GGA AGG GTC C-3’; Axin2: 5’-GAG TAG CGC CGT GTT AGT GAC T-3’ and 5’-CCA GGA AAG TCC GGA AGA GGT ATG-3’; Alp: 5’-CCA ACT CTT TTG TGC CAG AGA-3’ and 5’-GGC TAC ATT GGT GTT GAG CTT TT-3’, Rankl: 5’-CAG CAT CGC TCT GTT CCT GTA-3’ and 5’-CTG CGT TTT CAT GGA GTC TCA-3’, Itga11: 5’-TGC CCC AAT GGA AAC CAA TG-3’ and 5’-CAC TCG TGC GAC CAG AGA G-3’, Dmp1: 5’-TGG GAG CCA GAG AGG GTA G-3’ and 5’-TTG TGG TAT CTG GCA ACT GG-3’, Ctnnb1: 5’-CAT CTA CAC AGT TTG ATG CTG CT-3’ and 5’-GCA GTT TTG TCA GTT CAG GGA-3’. The primers used for qRT-PCR analysis of human RNA include: Osteolectin: 5’-ACA TCG TCA CTT ACA TCC TGG GC-3’ and 5’-CAC GCG GGT GTC CAA CG-3’; Actb: 5’-ATT GGC AAT GAG CGG TTC-3’ and 5’-CGT GGA TGC CAC AGG ACT-3’; Lef1: 5’-TGC CAA ATA TGA ATA ACG ACC CA-3’ and 5’-GAG AAA AGT GCT CGT CAC TGT-3’; Runx2: 5’-GAA CCC AGA AGG CAC AGA CA-3’ and 5’-GGC TCA GGT AGG AGG GGT AA-3’; Axin2: 5’- CAA CAC CAG GCG GAA CGA A-3’ and 5’- GCC CAA TAA GGA GTG TAA GGA CT-3’; Alp: 5’-GTG AAC CGC AAC TGG TAC TC-3’ and 5’-GAG CTG CGT AGC GAT GTC C-3’, Dmp1: 5’-CTC CGA GTT GGA CGA TGA GG-3’ and 5’-TCA TGC CTG CAC TGT TCA TTC-3’.

PCR genotyping

To genotype Itga11 floxed mice the following primers were used: 5’- AATTCAGTGCCGATCCTCCAGTGTC-3’, 5’-CCCTTGCTTCCTTCTGCTGTCACTT-3’ (Itga11fl allele: 370 bp; Itga11+ allele: 280 bp).

Integrin binding assay

Integrin binding assays were performed as described (Nishiuchi et al., 2006). Microtiter plates were coated with 10 nM recombinant human Osteolectin, recombinant human Pro-Collagen 1α, or Bovine Serum Albumin (BSA, Sigma A3156) overnight at 4°C, and then blocked with 10 mg/ml BSA. 6xHis tagged recombinant human integrin heterodimers were purchased from R and D Systems. The plates were incubated with integrins in TBS buffer (50 mM Tris-Cl, pH 7.5 150 mM NaCl) with 1 mM MnCl2, then washed with TBS containing 1 mM MnCl2, 0.1% BSA, and 0.02% Tween 20, followed by quantification of bound integrins by an enzyme-linked immunosorbent assay using an anti-His tag monoclonal antibody (Thermo Fisher Scientific, clone 4E3D10H2/E3) followed by a horseradish peroxidase-conjugated anti-mouse secondary antibody. After washing, bound HRP was detected using SureBlue TMB Microwell Peroxidase Substrate (KPL) and the reaction was stopped with TMB stop solution (KPL). The optical density was measured at 450 nm.

MicroCT analysis

MicroCT analysis was performed using the same settings as previously described (Yue et al., 2016). Based on previously described methods (Bouxsein et al., 2010), mouse femurs were dissected, fixed overnight in 4% paraformaldehyde (Thermo Fisher Scientific) and stored in 70% ethanol at 4°C. Femurs and lumbar vertebrae were scanned at an isotropic voxel size of 3.5 μm and 7 μm, respectively, with peak tube voltage of 55 kV and current of 0.145 mA (μCT 35; Scanco). A three-dimensional Gaussian filter (s = 0.8) with a limited, finite filter support of one was used to suppress noise in the images, and a threshold of 263–1000 was used to segment mineralized bone from air and soft tissues. Trabecular bone parameters were measured in the distal metaphysis of the femurs. The region of interest was selected from below the distal growth plate where the epiphyseal cap structure completely disappeared and continued for 100 slices toward the proximal end of the femur. Contours were drawn manually a few voxels away from the endocortical surface to define trabecular bones in the metaphysis. Cortical bone parameters were measured by analyzing 100 slices in mid-diaphysis femurs.

Statistical analysis

Numbers of experiments noted in figure legends reflect independent experiments performed on different days. Mice were allocated to experiments randomly and samples processed in an arbitrary order, but formal randomization techniques were not used. Prior to analyzing the statistical significance of differences among treatments we tested whether data were normally distributed and whether variance was similar among treatments. To test for normality, we performed the Shapiro–Wilk tests. To test whether variability significantly differed among treatments we performed F-tests (for experiments with two treatments) or Levene’s median tests (for experiments with more than two treatments). When the data significantly deviated from normality (p < 0.01) or variability significantly differed among treatments (p < 0.05), we log2-transformed the data and tested again for normality and variability. If the transformed data no longer significantly deviated from normality and equal variability, we then performed parametric tests on the transformed data. If the transformed data still significantly deviated from normality or equal variability, we performed non-parametric tests on the non-transformed data. Data from the same cell culture experiments were always paired for statistical analysis. Mouse littermates were paired for statistical analysis.

To assess the statistical significance of a difference between two treatments, we used paired two-tailed Student’s t-tests (when a parametric test was appropriate) or Wilcoxon’s tests (when a non-parametric test was appropriate). To assess the statistical significance of differences between more than two treatments, we used one-way or two-way repeated measures ANOVAs (when a parametric test was appropriate) followed by post-hoc tests including Dunnett’s, Sidak’s, and Tukey’s tests depending on the experimental settings and planned comparisons, or multiple Wilcoxon’s tests followed by Holm-Sidak’s method for multiple comparisons adjustment (when a non-parametric test was appropriate). Relative mRNA levels were always log2-transformed before any statistical tests were performed. All statistical analyses were performed with Graphpad Prism 7.02. All data represent mean ±standard deviation (*p < 0.05, **p < 0.01, ***p < 0.001).

Bone marrow digestion and CFU-F assay

As previously described (Yue et al., 2016), mouse femurs and tibias were cut at both ends to flush out intact marrow plugs. Both the flushed plugs and crushed bone metaphyses were subjected to two rounds of enzymatic digestion in prewarmed digestion buffer containing 3 mg/ml type I collagenase (Worthington), 4 mg/ml dispase (Roche Diagnostic) and 1 U/ml DNase I (Sigma) in HBSS with calcium and magnesium, at 37°C for 15 min each round. During each round of digestion, the suspension was vortexed six times for 10 s each time at speed level three using a Vortex-Genie two to promote more complete dissociation. Dissociated cells were transferred into a tube with staining medium (HBSS without calcium and magnesium +2% fetal bovine serum) and 2 mM EDTA to stop the digestion. Cells were then centrifuged, resuspended in staining medium, and passed through a 90 μm nylon mesh to filter undigested plugs or bone.

To form CFU-F colonies, freshly dissociated bone marrow cell suspensions were plated at clonal density in 6-well plates (5 × 105 cells/well) or 10 cm plates (5 × 106 cells/dish) with DMEM (Gibco) plus 20% fetal bovine serum (Sigma F2442), 10 mM ROCK inhibitor (Y-27632, Selleck), and 1% penicillin/streptomycin (Invitrogen) at 37°C in gas-tight chambers (Billups-Rothenberg) with 1% O2 and 6% CO2 (with balance Nitrogen) to maintain a low oxygen environment that promoted survival and proliferation (Morrison et al., 2000). The CFU-F culture dish was rinsed with HBBS without calcium and magnesium and replenished with freshly made medium on the second day after plating to wash out contaminating macrophages. Cultures were then maintained in a gas-tight chamber that was flushed daily for 1 min with a custom low oxygen gas mixture (1% O2, 6% CO2, balance Nitrogen). The culture medium was changed every 4 days. To count CFU-F colonies, the cultures were stained with 0.1% Toluidine blue in 4% formalin solution eight days after plating.

Differentiation assays in culture

The osteogenic potential of primary CFU-F cells, human bone marrow stromal cells, and MC3T3-E1 cells was assessed by plating the cells into 48- well plates (25,000 cells/cm2). On the second day after plating, the culture medium was replaced with osteogenic differentiation medium (StemPro Osteogenesis Differentiation kit, Gibco). Cells were maintained in the differentiation medium, with medium change every other day for 14 days for primary CFU-F cells and MC3T3-E1 cells before differentiation was assessed. For human bone marrow stromal cells, the culture medium was changed every 3 days for 21 days. Osteoblastic differentiation was detected by staining with Alizarin red S (Sigma). To quantitate Alizarin red staining, the stained cells were rinsed with PBS, and extracted with 10% (w/v) cetylpyridinium chloride in 10 mM sodium phosphate, pH 7.0 for 10 min at room temperature. Alizarin red in the extract was quantitated by optical density measurement at 562 nm.

The adipogenic potential of CFU-F cells was assessed by plating them into 48-well plates (25,000 cells/cm2). On the second day after plating, the culture medium was replaced with adipogenic differentiation medium (StemPro Adipogenesis Differentiation Kit, Gibco) and the cultures were allowed to differentiate for 14 days, with culture medium changed every 3 days. Adipocyte differentiation was detected by staining with Oil red O (Sigma). To quantitate the amount of Oil red O staining, cells were rinsed with PBS, and extracted with 100% isopropanol for 10 min at room temperature. Oil red O in the extract was quantitated by optical density measurement at 500 nm.

The chondrogenic potential of CFU-F cells was assessed by centrifuging 2 × 105 cells to form cell pellets, which were then cultured in chondrogenic medium (StemPro chondrogenesis differentiation kit; Gibco) for 21 days. The culture medium was changed every 3 days. Chondrocyte formation within the cell pellets was assessed by cryosectioning and Toluidine blue staining as described (Robey et al., 2014).

Calcein double labeling and histomorphometry analysis

As previously described (Egan et al., 2012), mice were injected intraperitoneally with 10 mg/kg body weight of calcein, dissolved in 0.15 M NaCl plus 2% NaHCO3 in water, at day 0 and day 7. Mice were sacrificed on day 9. Mouse tibias were fixed overnight in 4% paraformaldehyde at 4°C, dehydrated in 30% sucrose at 4°C for two days and sectioned without decalcification (7 µm sections). Mineral apposition rates were determined as previously described (Egan et al., 2012). The surface used to quantify the trabecular bone mineral apposition rate was 100 μm distal to the growth plate and 50 μm in from the endosteal cortical bone of the femur. The surface used to quantify cortical bone mineral apposition rate was the medial endosteal cortical bone surface of the femur.

ELISA assays

The bone resorption rate was determined by measuring urinary levels of deoxypyridinoline (DPD) using a MicroVue DPD ELISA Kit (Quidel). The DPD values were normalized to urinary creatinine levels using the MicroVue Creatinine Assay Kit (Quidel). The bone formation rate was determined by measuring serum levels of Procollagen type 1 N-terminal Propeptide (P1NP) using the Rat/Mouse P1NP ELISA kit (Immunodiagnostic Systems). The ELISA assay for Osteolectin was described previously (Yue et al., 2016).

Acknowledgements

SJM is a Howard Hughes Medical Institute (HHMI) Investigator, the Mary McDermott Cook Chair in Pediatric Genetics, the Kathryn and Gene Bishop Distinguished Chair in Pediatric Research, the director of the Hamon Laboratory for Stem Cells and Cancer, and a Cancer Prevention and Research Institute of Texas Scholar. BS was supported by a Ruth L. Kirschstein National Research Service Award (NRSA) Postdoctoral Fellowship (F32) from the National Heart, Lung, and Blood Institute (1F32HL139016-01). AT was supported by the Leopoldina Fellowship Program (LPDS 2016–16) from the German National Academy of Sciences. We thank Nicolas Loof and the Moody Foundation Flow Cytometry Facility, Albert Gross for mouse colony management, Yu Zhang and Hao Zhu for intracytoplasmic sperm injection. This work was supported by the National Institute on Aging (R37 AG02494514).

Funding Statement

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Contributor Information

Sean J Morrison, Email: sean.morrison@utsouthwestern.edu.

Didier Y Stainier, Max Planck Institute for Heart and Lung Research, Germany.

Clifford J Rosen, Maine Medical Center Research Institute, United States.

Funding Information

This paper was supported by the following grants:

  • National Heart, Lung, and Blood Institute 1F32HL139016-01 to Bo Shen.

  • German National Academy of Sciences LPDS 2016–16 to Alpaslan Tasdogan.

  • Howard Hughes Medical Institute to Sean J Morrison.

  • National Institutes of Health AG02494514 to Sean J Morrison.

Additional information

Competing interests

Senior editor, eLife.

No competing interests declared.

Was an inventor on a pending patent application claiming the use of Osteolectin to promote bone formation. The patent application has not been licensed, so has no existing financial interest in it. WO patent application number: WO2016172026A1.

Author contributions

Conceptualization, Formal analysis, Investigation, Writing—original draft, Writing—review and editing.

Investigation.

Investigation.

Investigation.

Formal analysis, Writing—review and editing.

Investigation.

Investigation.

Conceptualization, Resources, Formal analysis, Supervision, Funding acquisition, Writing—original draft, Project administration, Writing—review and editing.

Ethics

Animal experimentation: This study was performed in accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. All procedures were approved by the UT Southwestern Institutional Animal Care and Use Committee (protocol number 2016-101334-G).

Additional files

Supplementary file 1. Markers used for the flow cytometric isolation of bone marrow cell populations.

Stem and progenitor cell populations were isolated from mouse bone marrow by flow cytometry using the listed markers. Lineage (Lin) markers used to isolate Lineage negative cell populations were CD2, CD3, CD5, CD8, Ter119, Gr-1, and B220.

elife-42274-supp1.docx (111.5KB, docx)
DOI: 10.7554/eLife.42274.021
Transparent reporting form
DOI: 10.7554/eLife.42274.022

Data availability

Source data files have been provided for all figures.

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Decision letter

Editor: Clifford J Rosen1
Reviewed by: John Leesshepard2

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Thank you for submitting your article "Integrin α11 is an Osteolectin receptor and is required for the maintenance of adult skeletal bone mass" for consideration by eLife. Your article has been reviewed by three peer reviewers, and the evaluation has been overseen by Clifford Rosen as the Reviewing Editor and Didier Stainier as the Senior Editor. The following individual involved in review of your submission has agreed to reveal his identity: John Lees-Shepard (Reviewer #2).

As such, the reviewers have discussed the reviews with one another and the Reviewing Editor has drafted this decision to help you prepare a revised submission.

Overall there was consensus that the manuscript could break new ground in our understanding of adult stem cell fate and the mechanism of osteolectin in directing lineage through the Itga11 receptor. In general the experiments are illustrative and the results are relatively supportive of the conclusions. However there were several issues in your revision that need to be resolved before a final decision can be rendered. A major concern is the relatively mild skeletal phenotype with the LepRCre Itga11 at 12 months, the lack of studies with inhibitors to define the specificity of the integrin receptor, absence of any integrin signaling studies, concerns about confounding by collagen interactions and details about the recombinant osteolectin experiments in which there were inconsistent Wnt signaling. These issues can certainly be addressed in a revision with a greater 'n' for the in vitro studies, more phenotyping of the conditional model, and experiments related to the role of collagen in modulating integrin signaling, as well as competitive inhibitors for integrin signaling. A summary of the three reviewers comments are noted below:

Reviewer #1:

The authors continue their previous studies on Lepr+ stromal cells and the role of osteolectin in bone formation. Here they explored the receptor for osteolectin and provided evidence that integrin α11 is an osteolectin receptor mediating bone formation. A main concern has to do with the generally mild bone effect of Itga11 deletion by a constitutively active Cre (Lepr-Cre) that acts in not only osteoprogenitors but also many other cell types including adipocytes. The fact that the bone phenotype does not manifest until much later in life (12 months) makes it very difficult to discern any direct effect. This is an important point as it has become clear that bone mass is regulated by many other tissues outside the skeleton. Furthermore, one cannot discount the potential effect of the integrin deletion on collagen I binding in vivo which may in turn be responsible for the bone phenotype. Other comments are as follows.

"To refine potential candidate receptors for Osteolectin we more closelyexamined the expression of these genes in LepR+ cells, which are osteogenic and should express the receptor, and endothelial cells, which are not osteogenic and should not express the receptor. Itga1, Itga6, and ItgaV were strongly expressed by both LepR+ cells and endothelial cells, suggesting they did not encode the receptor (Figure 1F). Itga11 was expressed exclusively by LepR+ cells, not by endothelial cells or other bone marrow cells (Figure 1F)." The reasoning is not sound as there is no evidence that osteolectin/receptor signaling alone is sufficient to induce osteogenesis. Therefore being expressed in nonosteogenic cells should not be an exclusion criterion for the potential receptor.

Details about production and application of recombinant osteolectin and pro-Collagen I used in Figure 1K are missing. Were collagen I used to coat the culture plates, which may be necessary to engage the integrins? Some testing of integrin activation is necessary as a positive control in order for any conclusions to be drawn from a negative result here. Same applies to Figure 4D and 4E. Also in Materials and methods, details about in vivo application of recombinant osteolectin (dose, age of mice etc.) is missing.

Figure 2B, Alizarin red staining alone is not sufficient to support claims about OB differentiation especially since the authors deleted an extracellular matrix protein that may directly affect nucleation of minerals. Same criticism applies to Figure 4A, 7C, 7F.

Figure 2B, the authors observed no increase in β-catenin within 1 hr of osteolectin treatment but only after 24 hrs. This data argues against a direct effect of osteolection on β-catenin signaling. Similarly, the role of β-catenin in osteolectin-induced osteogenesis is also likely indirect. Considering the known function of β-catenin in osteoblast differentiation it is expected that β-catenin depletion would suppress osteogenesis by any upstream osteogenic signals.

Figure 6. The authors show no change in cortical total area (6B) or cortical area (6C) but a significant decrease in cortical thickness (6D) and Ct Ar/Tt Ar (6E). How do they explain that? Figure 6G, what is the surface used for MAR measurements? In general, how did the authors control for effects of genetic background variations on the parameters?

On multiple occasions, the authors seem to imply MC3T3-E1 cells as bone marrow stromal cells, but they are cell lines derived from the newborn mouse calvaria, and generally considered to be more advanced along the OB lineage than the BM stromal cells. These should be corrected. " MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells secrete Osteolectin into the culture medium (Figure 2A), consistent with our observation that Osteolectin is synthesized by a subset of LepR+ bone marrow stromal cells (Yue et al., 2016)." "Addition of recombinant Osteolectin to culture significantly promoted osteogenesis by parental, but not Itga11 deficient, MC3T3-E1,hBMSC#1, and hBMSC#2 cells (Figure 4A). Integrin α11 is therefore required by mouse and human bone marrow stromal cells to undergo osteogenesis in response to Osteolectin." "Integrin α11 is therefore required by mouse and human bone marrow stromal cells to activate Wnt pathway signaling in response to Osteolectin."

Reviewer #2:

This is a well-written paper in which the authors build upon their previous work by identifying Integrin α11 as a Clec11a/Osteolectin receptor. Further, the authors provide evidence that the anabolic effect of Osteolectin occurs via activation of the Wnt pathway in osteogenic cells. The experiments in this paper are generally well-described, well-documented, and convincingly presented. This paper is appropriate for publication in eLife as it significantly advances understanding with regard to factors that regulate adult bone homeostasis.

Reviewer #3:

Shen et al. demonstrated that osteolectin contributes to osteogenesis via integrin α11 using biochemical and cellular assays as well as genetical deletion of the integrin in mouse model. It is new to identify integrin α11β1 as an osteolectin receptor and the genetical deletion of integrin α11 phenocopies the osteolectin knockout.

Main discovery is that integrin α11β1 is the osteolectin receptor, which was shown by protein-protein interaction assay. The assay was performed well but the competition assay targeting the interaction of integrin-ligand will intensify the claim. The author developed the hypothesis based on the conserved peptide such as RGD and LDT. There are several inhibitors based on the RGD peptide. Using the inhibitors, the author also can test whether the interaction between two proteins is crucial for osteogenesis in a cell culture experiment. The inhibitors can be useful in vivo experiment as well. The author mainly shows the phenotype of integrin α11 knockout or correlation of β-catenin protein level instead of the interaction between osteolectin and the integrin. Considering the main theme of the paper, I recommend in vivo phenotype test using the ligand and its inhibitor to confirm the functional roles of the interaction between osteolectin and integrin α11β1.

The important feature of Integrin-ligand binding is the phosphorylation of focal adhesion kinase (FAK) at Y397. The phosphorylation initiates integrin-mediated canonical signaling upon ligand binding. However, the author didn't show the level or any description to explain how the interaction initiates downstream signaling. The author used collagen as a control, which is the ligand but not for the osteogenesis/stabilization of β-catenin. Using the level of phosphorylated FAK, the author can show that collagen is active for integrin-ligand binding but the interaction doesn't contribute to osteogenesis.

Another main point is that osteolectin-integrin αV system activates β-catenin signaling to increase osteogenesis. The author showed that 24 hour-treatment of osteolectin stabilizes β-catenin in cells while phosphorylation of GSK3 happened within an hour. The function of β-catenin is well known to be a transcriptional activator but it is also important to maintain cell-cell junction with E-cadherin. The first β-catenin resides in the cytoplasm and the inhibitor of proteasomal degradation of the protein can increase the protein level, which allows the protein to enter the nucleus to activate transcriptional factors. However, the second β-catenin is in the cell membrane and it is not clear whether the level is regulated by the same proteasomal degradation pathway. Therefore, it is necessary to distinguish the levels of β-catenin in cytosol/nucleus from the levels in the membrane by biochemical separation or immunohistochemistry, which will clarify the result. Since author used 24 hours for detection of enhanced β-catenin protein level which is abnormally long compared to Wnt (1-2 hours), it is also necessary to prove that it is from proteasomal degradation not from transcriptional effects or et al. I'd like to suggest pulse-chase of the protein. Additionally, I suggest other time points such as 2 and 4 hours after osteolectin treatment as Wnt treatment experiments.

eLife. 2019 Jan 11;8:e42274. doi: 10.7554/eLife.42274.026

Author response


Overall there was consensus that the manuscript could break new ground in our understanding of adult stem cell fate and the mechanism of osteolectin in directing lineage through the Itga11 receptor. In general the experiments are illustrative and the results are relatively supportive of the conclusions. However there were several issues in your revision that need to be resolved before a final decision can be rendered. A major concern is the relatively mild skeletal phenotype with the LepRCre Itga11 at 12 months, the lack of studies with inhibitors to define the specificity of the integrin receptor, absence of any integrin signaling studies, concerns about confounding by collagen interactions and details about the recombinant osteolectin experiments in which there were inconsistent Wnt signaling. These issues can certainly be addressed in a revision with a greater 'n' for the in vitro studies, more phenotyping of the conditional model, and experiments related to the role of collagen in modulating integrin signaling, as well as competitive inhibitors for integrin signaling. A summary of the three reviewers comments are noted below:

We thank the reviewers for their thoughtful comments. We provide detailed responses to specific reviewers below, but summarize our responses to the major issues here:

a) “relatively mild skeletal phenotype with the LepRCre Itga11 at 12 months”

We address this issue in detail in response to reviewer #1. The LepR+ cells do not contribute to bone formation during skeletal development or early postnatal growth. LepR+ cells only begin to contribute to skeletal bone during adulthood (Cell Stem Cell 15:154). Therefore, conditional deletion of Itga11 with Lepr-cre would not be expected to yield a phenotype at 2 months of age, when only around 5% of osteoblasts derive from LepR+ cells (Cell Stem Cell 15:154). The phenotype would not be expected to be apparent until 6-12 months of age, when the LepR+ cells are a major source of new osteoblasts (Cell Stem Cell 15:154). We have added new data from four additional pairs of Lepr-cre; Itga11fl/fl and sex-matched littermate controls at 12 months of age. The data show that trabecular bone volume and mineral apposition rate were significantly reduced in male and female Lepr-cre; Itga11fl/fl mice relative to controls at 6 and 12 months of age. Cortical bone mineral density and mineral apposition rate were significantly reduced in male and female Lepr-cre; Itga11fl/fl mice relative to controls at 12 months of age. Therefore, both trabecular and cortical bone were significantly reduced in Lepr-cre; Itga11fl/fl mice. The later onset of the cortical bone phenotype as compared to the trabecular bone phenotype was also observed for the Osteolectin germline knockout mouse (eLife 5:e18782). Therefore, the Lepr-cre; Itga11fl/fl mice phenocopy not only the bone loss observed in Osteolectin deficient mice but also the difference in timing of trabecular versus cortical bone loss.

b) “lack of studies with inhibitors to define the specificity of the integrin receptor”

We address this below in response to reviewers #1 and #3. We added new data showing that an inhibitor of integrin binding, RGDS peptide, blocked Osteolectin binding to integrin α11β1 (new Figure 1L) and the ability of Osteolectin to induce osteogenic differentiation (new Figure 1M).

c) “absence of any integrin signaling studies”

We address this below in response to reviewer #3. We added new data showing that Osteolectin activates integrin signaling by promoting the phosphorylation of Focal Adhesion Kinase (FAK) at Y397 (new Figure 4G and 4H), in addition to promoting the phosphorylation of GSK3 at Ser21/9 (Figure 2D and Figure 4G and 4H).

d) “concerns about confounding by collagen interactions”

We address this in detail in response to reviewer #1. We did not observe any effect of exogenous collagen on osteogenic differentiation (Figure 1K), and deletion of integrin a11 would be unlikely to block the effects of collagen on LepR+ cells since LepR+ cells express multiple other collagen receptors.

e) “details about the recombinant osteolectin experiments in which there were inconsistent Wnt signaling”

We address this below in response to Reviewer #1, point #1. We added additional experiments testing the effects of Osteolectin treatment on the transcription of Wnt target genes. The effects are now more consistently statistically significant across experiments.

Reviewer #1:

The authors continue their previous studies on Lepr+ stromal cells and the role of osteolectin in bone formation. Here they explored the receptor for osteolectin and provided evidence that integrin α11 is an osteolectin receptor mediating bone formation. A main concern has to do with the generally mild bone effect of Itga11 deletion by a constitutively active Cre (Lepr-Cre) that acts in not only osteoprogenitors but also many other cell types including adipocytes.

LepR+ cells do not contribute to bone formation during skeletal development or early postnatal growth. LepR+ cells arise postnatally and do not begin to contribute to bone formation until adulthood (Cell Stem Cell 15:154). Therefore, conditional deletion of Itga11 with Lepr-cre would not be expected to yield a phenotype at 2 months of age, when only around 5% of osteoblasts derive from LepR+ cells (Cell Stem Cell 15:154). The phenotype would be expected to become detectable by 6 months of age, when the LepR+ cells are a major source of new osteoblasts for skeletal maintenance. The defects observed in the Lepr-cre; Itga11fl/fl mice phenocopy the defects observed in Osteolectin deficient mice (eLife 5:e18782):

We have characterized the Lepr-cre recombination pattern in detail (Cell Stem Cell 15:154) and do not observe recombination in other cell types in the bone marrow, where Osteolectin is expressed. Lepr-cre recombines in only 0.3% of bone marrow cells. These cells do give rise to adipocytes, but adipocytes are rare in the femurs of young adult mice (the bones we studied) (Cell Stem Cell 15:154). Moreover, adipocytes do not express Itga11, as shown in the RNA-seq data in Author response table 1. In light of our in vitro data demonstrating that Itga11 deficiency acts within LepR+ stromal cells to block the osteogenic response to Osteolectin (Figure 7F and 7G), there is no reason to think that the in vivo effects of Osteolectin are instead mediated through an indirect mechanism involving adipocytes.

Author response table 1. RNA-seq analysis of LepR+ cells from non-irradiated bone marrow as well as hematopoietic cells and unfractionated cells from irradiated bone marrow (which are high in adipocytes).

Itga11 is poorly expressed in adipocyte-rich bone marrow cells after irradiation (all data represent average FPKM values from multiple replicates).

Cell type Adipoq Fabp4 Plin1 Itga11 Osteolectin
LepR+ cells from bone marrow 835 23 1.2 34 37
Hematopoietic cells from irradiated bone marrow 1.8 13 0.3 0.1 0.2
Whole bone marrow cells after irradiation (high in adipocytes) 92 1016 68 2.2 2.8

The fact that the bone phenotype does not manifest until much later in life (12 months) makes it very difficult to discern any direct effect. This is an important point as it has become clear that bone mass is regulated by many other tissues outside the skeleton. Furthermore, one cannot discount the potential effect of the integrin deletion on collagen I binding in vivo which may in turn be responsible for the bone phenotype. Other comments are as follows.

The bone phenotype manifests before 6 months of age. We observed a significant reduction in trabecular bone volume (Figure 5G) and trabecular mineral apposition rate (Figure 5M) in the Lepr-cre; Itga11fl/fl mice at 6 months of age. Since we would not expect to observe a phenotype until after 2 months of age (see above), and 6 months was the next time point we analyzed, we believe the phenotype became evident in young adult mice, just as would be expected based on the Osteolectin deficiency phenotype (eLife 5:e18782).

Although many tissues outside the skeleton can influence bone mass, the expression patterns of Lepr-cre and Itga11 are each very restricted. We believe that only osteogenic progenitors express both genes. Moreover, the in vitro data demonstrate a direct effect of Osteolectin on the osteogenic differentiation of LepR+ bone marrow stromal cells in culture (Figure 7F and 7G). This effect was completely eliminated when Itga11 was deleted from LepR+ bone marrow stromal cells (Figure 7F and 7G) or from a pre-osteoblast cell line or human bone marrow stromal cells (Figure 4A and 4B). The only conclusion that is consistent with all of the in vitro and in vivo data is that integrin a11 is required in mesenchymal progenitors for the osteogenic response to Osteolectin.

We were unable to detect an effect of exogenous collagen on osteogenesis by three preosteoblast or bone marrow stromal cell lines (Figure 1K). Collagen binding is mediated by several integrins, including a1b1, a2b1, a10b1, and aVb3 (Prog Histochem Cytochem 37:3 and Biochem Biophys Res Commun 182:1025). Since multiple collagen receptors, including a1b1, a10b1 and aVb3, are expressed by bone marrow stromal cells (Figure 1D and 1E), the loss of one integrin (a11) would likely not be sufficient to block the response of these cells to collagen. Even if a11 deficiency had some effect on collagen binding, this would not explain the loss of Osteolectin responsiveness by isolated mesenchymal cells in culture.

"To refine potential candidate receptors for Osteolectin we more closelyexamined the expression of these genes in LepR+ cells, which are osteogenic and should express the receptor, and endothelial cells, which are not osteogenic and should not express the receptor. Itga1, Itga6, and ItgaV were strongly expressed by both LepR+ cells and endothelial cells, suggesting they did not encode the receptor (Figure 1F). Itga11 was expressed exclusively by LepR+ cells, not by endothelial cells or other bone marrow cells (Figure 1F)." The reasoning is not sound as there is no evidence that osteolectin/receptor signaling alone is sufficient to induce osteogenesis. Therefore being expressed in nonosteogenic cells should not be an exclusion criterion for the potential receptor.

We have revised the quoted text to soften the assumption that the Osteolectin receptor would not be expressed by endothelial cells. The expression data were only a way of identifying candidate integrins that might mediate the effect of Osteolectin on osteogenic progenitors. All of our conclusions were based on functional assays that demonstrated that Osteolectin binds α11β1 (Figure 1I and 1J), that a11 is required for the osteogenic response of LepR+ cells to Osteolectin in culture (Figure 7F and 7G, Figure 4A and 4B), and that conditional deletion of Itga11 in LepR+ cells in vivo phenocopies the effects of Osteolectin deficiency on adult skeletal bone mass (Figure 5F-M and Figure 6A-G). We were unable to detect Osteolectin binding to integrins other than α11β1 and α10β1 (Figure 1I). Nonetheless, we agree that there could potentially be additional Osteolectin receptors that we did not observe, and noted that in the discussion of the manuscript.

Details about production and application of recombinant osteolectin and pro-Collagen I used in Figure 1K are missing.

We have added more detail to the Materials and methods to address this. We purchased recombinant His-tagged human Pro-Collagen 1α from R&D Systems and removed the His tag using TEV protease. After cleavage, we purified the untagged Pro-Collagen 1α using Ni-NTA agarose columns to separate it from the cleaved His tag, the His-tagged Pro-Collagen 1α, and the His-tagged TEV protease.

Was collagen I used to coat the culture plates, which may be necessary to engage the integrins? Some testing of integrin activation is necessary as a positive control in order for any conclusions to be drawn from a negative result here. Same applies to Figure 4D and 4E.

In the data shown in the manuscript, Pro-Collagen 1α or Osteolectin was added to the supernatant of the culture medium. We have added new data to the revised manuscript showing that when Pro-Collagen 1α or Osteolectin was added to the culture medium, each activated integrin signaling in mouse bone marrow stromal cells based on increased FAK and GSK3 phosphorylation (Figure 4G and 4H). However, Osteolectin promoted the accumulation of nuclear β-catenin (Figure 4H and 4I) and osteogenic differentiation (Figure 1K) while ProCollagen 1α did not (Figure 4H, 4I, and 1K). These data suggest that when added to the culture medium, both Pro-Collagen 1α and Osteolectin activate integrin signaling but that Osteolectin is more effective at promoting β-catenin accumulation, Wnt target gene expression, and osteogenic differentiation.

We also tried coating cell culture dishes with Pro-Collagen 1α or Osteolectin before seeding the osteogenic cells. However, when we coated the dishes, neither Pro-Collagen 1α nor Osteolectin promoted osteogenic differentiation.

Also in Materials and methods, details about in vivo application of recombinant osteolectin (dose, age of mice etc.) is missing.

We have added more detail to the Materials and methods to address this (see the ‘Recombinant protein production and application’ section). For in vivo use of recombinant mouse Osteolectin, 50 µg/kg body mass of recombinant Osteolectin was subcutaneously injected daily into 2month-old female Lepr-cre; Itga11fl/fl or littermate control mice for 28 days.

Figure 2B, Alizarin red staining alone is not sufficient to support claims about OB differentiation especially since the authors deleted an extracellular matrix protein that may directly affect nucleation of minerals. Same criticism applies to Figure 4A, 7C, 7F.

We have added qRT-PCR data to the manuscript showing that the increase in Alizarin red staining in those experiments was also associated with increased transcription of Dmp1 (see new Figures 2C, 4B, and 7G), a marker of bone formation (JBMR 17-1822).

Figure 2B, the authors observed no increase in β-cat within 1 hr of osteolectin treatment but only after 24 hrs. This data argues against a direct effect of osteolection on β-catenin signaling. Similarly, the role of β-catenin in osteolectin-induced osteogenesis is also likely indirect. Considering the known function of β-catenin in osteoblast differentiation it is expected that β-catenin depletion would suppress osteogenesis by any upstream osteogenic signals.

The time required to detect nuclear β-catenin accumulation after Wnt pathway activation varies among cells and assay conditions. Although some cell types do it within an hour, other cell types (or assay conditions) require more time (J Bone Miner Res 31:2215, Clin Cancer Res 18:4997, Arterioscler Thromb Vasc Biol 34:2268). In the original manuscript, we performed western blots at only 1 and 24 hours, so we knew there was an increase in nuclear β-catenin by 24 hours after Osteolectin treatment but had not done intermediate time points to assess when this was first evident. We have now added new data to the manuscript demonstrating that the increase in nuclear β-catenin was evident within 4-6 hours of Osteolectin treatment (new Figure 4I).

Published studies suggest that β-catenin directly promotes the expression of Wnt pathway target genes that are required for osteogenic differentiation including Alkaline phosphatase, Axin2, Lef1, and Runx2 (Cell 107:513, Mol Cell Biol 22:1172, Nature 382:638, and J. Biol. Chem. 280:33132,). We observed significantly increased transcription of each of these genes, in an a11-dependent manner, after treatment of mesenchymal stem/progenitor cells with Osteolectin in culture (Figure 4D and 4F) and in vivo (Figure 7—figure supplement 1B). The fact that we observe these effects in culture in multiple osteogenic cell lines, as well as primary mouse bone marrow stromal cells, and that the effects of Osteolectin on Wnt target gene expression are abolished by Itga11 deficiency, strongly argues that Osteolectin/α11β1 signaling directly promotes Wnt pathway activation.

We agree that β-catenin depletion would be expected to block the effects of other osteogenic signals. However, this does not undermine any of our conclusions. The known role of β-catenin in osteogenesis is consistent with our conclusion that Osteolectin promotes osteogenesis at least partly by activating β-catenin. Our data showing that Osteolectin promotes nuclear accumulation of β-catenin (new Figure 4I) and that treatment with IWR-1-endo (a drug that promotes β-catenin degradation) blocks the effect of Osteolectin on osteogenesis (Figure 3C and 3D) suggest that Osteolectin promotes osteogenesis at least partly by activating the Wnt pathway. Other signaling mechanisms may also contribute to osteogenesis in response to Osteolectin but our data show that the increase in β-catenin is necessary. The literature indicates that multiple mechanisms contribute to Wnt pathway activation in osteogenic progenitors (J. Clin. Invest. 116:1202), so there is no reason to think that Osteolectin could not be among them. We have added text to the Discussion more clearly explaining this.

Figure 6. The authors show no change in cortical total area (6B) or cortical area (6C) but a significant decrease in cortical thickness (6D) and Ct Ar/Tt Ar (6E). How do they explain that? Figure 6G, what is the surface used for MAR measurements? In general, how did the authors control for effects of genetic background variations on the parameters?

We have added new data from four additional pairs of Lepr-cre; Itga11fl/fl and sex-matched littermate controls at 12 months of age. Now there are no statistically significant differences in cortical total area (Figure 6B), cortical area (Figure 6C), cortical thickness (Figure 6D) or Ct Ar/Tt Ar (Figure 6E). However, Lepr-cre; Itga11fl/fl mice do exhibit significantly reduced cortical bone mineral density (Figure 6F) and cortical mineral apposition rate (Figure 6G) at 12 months of age. Osteolectin deficient mice also exhibited a milder cortical bone loss as compared to trabecular bone loss, which we only detected in the original manuscript at 16 months of age (eLife 5:e18782); therefore, the later onset and milder cortical bone phenotype in Lepr-cre; Itga11fl/fl mice phenocopy what we observed in Osteolectin deficient mice.

In Figure 6G, the surface used to quantify the cortical mineral apposition rate was the medial endosteal cortical surface of the femur, as previous described (Egan et al., 2012). To avoid effects of genetic background variation, we generated the Itga11 floxed allele by CRISPR-targeting in pure C57BL/Ka embryos. We confirmed germline transmission of the allele by mating with C57BL/Ka mice, then back-crossed the mice for at least 3 generations to C57BL/Ka wild-type mice prior to analysis of the phenotype. The Lepr-cre mice had also been backcrossed for at least 8 generations onto a C57BL/Ka background. Therefore, no mixed genetic backgrounds were used in our experiments. We have revised the Materials and methods to provide this additional detail.

On multiple occasions, the authors seem to imply MC3T3-E1 cells as bone marrow stromal cells, but they are cell lines derived from the newborn mouse calvaria, and generally considered to be more advanced along the OB lineage than the BM stromal cells. These should be corrected. " MC3T3-E1 cells, hBMSC#1 cells, and hBMSC#2 cells secrete Osteolectin into the culture medium (Figure 2A), consistent with our observation that Osteolectin is synthesized by a subset of LepR+ bone marrow stromal cells (Yue et al., 2016)." "Addition of recombinant Osteolectin to culture significantly promoted osteogenesis by parental, but not Itga11 deficient, MC3T3-E1,hBMSC#1, and hBMSC#2 cells (Figure 4A). Integrin α11 is therefore required by mouse and human bone marrow stromal cells to undergo osteogenesis in response to Osteolectin." "Integrin α11 is therefore required by mouse and human bone marrow stromal cells to activate Wnt pathway signaling in response to Osteolectin."

Thank you for catching this error, we have corrected this.

Reviewer #3:

Shen et al. demonstrated that osteolectin contributes to osteogenesis via integrin α11 using biochemical and cellular assays as well as genetical deletion of the integrin in mouse model. It is new to identify integrin α11β1 as an osteolectin receptor and the genetical deletion of integrin α11 phenocopies the osteolectin knockout.

Main discovery is that integrin α11β1 is the osteolectin receptor, which was shown by protein-protein interaction assay. The assay was performed well but the competition assay targeting the interaction of integrin-ligand will intensify the claim. The author developed the hypothesis based on the conserved peptide such as RGD and LDT. There are several inhibitors based on the RGD peptide. Using the inhibitors, the author also can test whether the interaction between two proteins is crucial for osteogenesis in a cell culture experiment. The inhibitors can be useful in vivo experiment as well. The author mainly shows the phenotype of integrin α11 knockout or correlation of β-catenin protein level instead of the interaction between osteolectin and the integrin. Considering the main theme of the paper, I recommend in vivo phenotype test using the ligand and its inhibitor to confirm the functional roles of the interaction between osteolectin and integrin α11β1.

Good idea. We have added new data demonstrating that RGDS peptide (Arg-Gly-

Asp-Ser), a potent antagonist of integrin binding (Cell 42:439), inhibited the binding of Osteolectin to integrin α11β1 (new Figure 1L) and blocked the osteogenic response of MC3T3E1 cells and bone marrow stromal cells to Osteolectin in culture (new Figure 1M). We performed these experiments in culture, rather than in vivo, because it would be necessary to inhibit the interaction of Osteolectin with its receptor in vivo for at least a month to detect an effect on osteogenesis. RGDS peptide binds to many other integrins and disrupts many biological processes in vivo, such as the function of integrin αIIbβ3 on platelets and integrins on leukocytes and endothelial cells (Thromb J 15:22, Blood 113:4078 and Cancer Res 62:5139). This would make it challenging to dose the mice with high enough levels of RGDS over a long enough time to detect effects on osteogenesis without causing other serve phenotypes (such as changes in feeding or body mass) that might confound our ability to interpret the results.

The important feature of Integrin-ligand binding is the phosphorylation of focal adhesion kinase (FAK) at Y397. The phosphorylation initiates integrin-mediated canonical signaling upon ligand binding. However, the author didn't show the level or any description to explain how the interaction initiates downstream signaling. The author used collagen as a control, which is the ligand but not for the osteogenesis/stabilization of β-catenin. Using the level of phosphorylated FAK, the author can show that collagen is active for integrin-ligand binding but the interaction doesn't contribute to osteogenesis.

Another good idea, thank you. We have now added new data to the manuscript showing that Osteolectin treatment of mouse bone marrow stromal cells promotes FAK phosphorylation at Y397 to a similar extent as Pro-Collagen 1α (new Figure 4G and 4H). This demonstrates that, although both Osteolectin and Pro-Collagen 1α bound to integrin α11β1 (Figure 1J) and initiated integrin signaling (new Figure 4G and 4H), only Osteolectin promoted nuclear β-catenin accumulation and osteogenic differentiation (new Figure 4H and 4I).

Another main point is that osteolectin-integrin αV system activates β-catenin signaling to increase osteogenesis. The author showed that 24 hour-treatment of osteolectin stabilizes β-catenin in cells while phosphorylation of GSK3 happened within an hour. The function of β-catenin is well known to be a transcriptional activator but it is also important to maintain cell-cell junction with E-cadherin. The first β-catenin resides in the cytoplasm and the inhibitor of proteasomal degradation of the protein can increase the protein level, which allows the protein to enter the nucleus to activate transcriptional factors. However, the second β-catenin is in the cell membrane and it is not clear whether the level is regulated by the same proteasomal degradation pathway. Therefore, it is necessary to distinguish the levels of β-catenin in cytosol/nucleus from the levels in the membrane by biochemical separation or immunohistochemistry, which will clarify the result. Since author used 24 hours for detection of enhanced β-catenin protein level which is abnormally long compared to Wnt (1-2 hours), it is also necessary to prove that it is from proteasomal degradation not from transcriptional effects or et al. I'd like to suggest pulse-chase of the protein. Additionally, I suggest other time points such as 2 and 4 hours after osteolectin treatment as Wnt treatment experiments.

We have added new data to the manuscript in which we treated mouse bone marrow stromal cells with PBS or recombinant Osteolectin then assessed β-catenin levels in the nuclear fraction or the cytosolic/membrane fraction 2, 4, 6, 12, and 24 hours later (new Figure 4I). Osteolectin treatment had no effect on β-catenin levels in the cytosolic/membrane fraction at any time point. However, Osteolectin did significantly increase β-catenin levels in the nuclear fraction after 4, 6, 12, and 24 hours. We also assessed Ctnnb1 (which encodes β-catenin) transcript levels in the same samples by qRT-PCR and observed no effect of Osteolectin on Ctnnb1 transcription 6 or 24 hours after Osteolectin treatment (Figure 4J). These data suggest that Osteolectin promotes nuclear β-catenin accumulation within 4 hours in bone marrow stromal cells by inhibiting proteasomal degradation, consistent with Wnt pathway activation.

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    Supplementary Materials

    Figure 1—source data 1. Data for Figure 1.
    DOI: 10.7554/eLife.42274.004
    Figure 2—source data 1. Data for Figure 2.
    DOI: 10.7554/eLife.42274.006
    Figure 3—source data 1. Data for Data for Figure 3.
    DOI: 10.7554/eLife.42274.008
    Figure 4—source data 1. Data for Figure 4.
    DOI: 10.7554/eLife.42274.010
    Figure 5—source data 1. Data for Data for Figure 5.
    DOI: 10.7554/eLife.42274.012
    Figure 5—figure supplement 1—source data 1. Data for Figure 5—figure supplement 1.
    DOI: 10.7554/eLife.42274.014
    Figure 6—source data 1. Data for Figure 6.
    DOI: 10.7554/eLife.42274.016
    Figure 7—source data 1. Data for Figure 7.
    DOI: 10.7554/eLife.42274.018
    Figure 7—figure supplement 1—source data 1. Data for Figure 7—figure supplement 1.
    DOI: 10.7554/eLife.42274.020
    Supplementary file 1. Markers used for the flow cytometric isolation of bone marrow cell populations.

    Stem and progenitor cell populations were isolated from mouse bone marrow by flow cytometry using the listed markers. Lineage (Lin) markers used to isolate Lineage negative cell populations were CD2, CD3, CD5, CD8, Ter119, Gr-1, and B220.

    elife-42274-supp1.docx (111.5KB, docx)
    DOI: 10.7554/eLife.42274.021
    Transparent reporting form
    DOI: 10.7554/eLife.42274.022

    Data Availability Statement

    Source data files have been provided for all figures.


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