Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent(M.musculus) | Osteolectin-/- | PMID: 27976999 | ||
| Genetic reagent (M.musculus) | Lepr-Cre | PMID: 11283374 | JAX Stock (008320) | |
| Antibody | rabbit polyclonal anti-phospho-PI3 Kinase | Cell Signaling | 4228S | (1:1000) |
| Antibody | rabbit polyclonal anti-phospho-Akt (Ser473) | Cell Signaling | 4060S | (1:1000) |
| Antibody | rabbit polyclonal anti-phospho-GSK-3α/β | Cell Signaling | 9331S | (1:1000) |
| Antibody | rabbit polyclonal anti-β-Catenin | Cell Signaling | 8480S | (1:1000) |
| Antibody | rabbit polyclonal anti-anti-β-Actin | Cell Signaling | 8457S | (1:10000) |
| Antibody | rabbit polyclonal anti-GSK-3b | Cell Signaling | 9315S | (1:1000) |
| Antibody | goat polyclonal anti-rabbit IgG, HRP-linked antibody | Cell Signaling | 7074S | (1:5000) |
| Antibody | horse polyclonal anti-mouse IgG, HRP-linked antibody | Cell Signaling | 7076S | (1:5000) |
| Antibody | donkey polyclonal anti-sheep IgG, HRP-linked antibody | R and D Systems | HAF016 | (1:5000) |
| Antibody | rabbit polyclonal anti-phospho-FAK (Y397) | Cell Signaling | 3283S | (1:1000) |
| Antibody | rabbit polyclonal anti-Histone H3 | Cell Signaling | 4499S | (1:5000) |
| Antibody | goat polyclonal anti-mouse Osteolectin | R and D Systems | AF3729 | (1:1000) |
| Antibody | sheep polyclonal anti-human Osteolectin | R and D Systems | AF1904 | (1:1000) |
| Antibody | rabbit polyclonal anti-Adiponectin | Abcam | ab181699 | (1:1000) |
| Antibody | rabbit polyclonal anti-Integrin α11 | Abcam | ab198826 | (1:1000) |
| Antibody | rabbit polyclonal anti-FAK | Cell Signaling | 3285P | (1:1000) |
| Antibody | mouse monoclonal anti-6x-His Tag antibody | Thermo Fisher Scientific | MA1-135 | (1:1000) |
| Antibody | rat polyclonal anti-CD45-APC | Tonbo | 20–0451 | (1:200) |
| Antibody | rat polyclonal anti-Ter119-APC | Tonbo | 20–5921 | (1:200) |
| Antibody | rat polyclonal anti-CD31-APC | Biolegend | 102410 | (1:200) |
| Antibody | goat polyclonal anti-Mouse Leptin R, Biotin | R and D Systems | AF497 | (1:200) |
| Antibody | mouse monoclonal anti-Streptavidin PE | Biolegend | 410504 | (1:500) |
| Peptide, recombinant protein | Bovine serum albumin | Sigma-Aldrich | A3156 | |
| Peptide, recombinant protein | recombinant human pro-Collagen I α1 | R and D Systems | 6220 CL | |
| Peptide, recombinant protein | recombinant human osteolectin | PMID: 27976999 | ||
| Peptide, recombinant protein | recombinant mouse osteolectin | PMID: 27976999 | ||
| Peptide, recombinant protein | recombiant Integrin α11β1 protein | R and D Systems | 6357-AB | |
| Peptide, recombinant protein | recombiant Integrin α10β1 protein | R and D Systems | 5895-AB | |
| Peptide, recombinant protein | recombiant Integrin αVβ3 protein | R and D Systems | 3050-AV | |
| Peptide, recombinant protein | recombiant Integrin αVβ1 Protein | R and D Systems | 6579-AV | |
| Peptide, recombinant protein | recombiant Integrin α4β1 protein | R and D Systems | 5668-A4 | |
| Peptide, recombinant protein | recombiant Integrin α9β1 protein | R and D Systems | 5438-A9 | |
| Peptide, recombinant protein | recombiant Integrin αIIbβ3 Protein | R and D Systems | 7148-A2 | |
| Peptide, recombinant protein | recombiant Integrin αMβ2 Protein | R and D Systems | 4047-AM | |
| Chemical compound,drug | DAPI | Life Technologies | D1306 | |
| Chemical compound, drug | TRIzol LS Reagent | Invitrogen | 10296028 | |
| Chemical cmpound,drug | Collagenase, Type 1 | Worthington | LS004196 | |
| Chemical compound, drug | Dispase II | Roche Diagnostic | D4693 | |
| Chemical compound, drug | DNase I | Sigma-Aldrich | 10 104 159 001 | |
| Chemical compound, drug | IWR-1-endo | Sigma-Aldrich | 681669 | |
| Chemical compound, drug | AZD2858 | Selleck | S7253 | |
| Chemical compound, drug | Y-27632 Rock inhibitor | Selleck | S1049 | |
| Chemical compound, drug | 4% paraformaldehyde in PBS | Thermo Fisher Scientific | J19943-K2 | |
| Cell lines (M.musculus) | MC3T3-E1, subclone 4 | ATCC | CRL-2593 | |
| Cell lines (H.sapiens) | hBMSC#1 | ATCC | PCS-500–012 | |
| Cell lines (H.sapiens) | hBMSC#2 | Lonza | PT-2501 | |
| Commercial assay or kit | Cell Culture Contamination Detection Kit | Molecular Probes | C-7028 | |
| Commercial assay or kit | TMB stop solution | KPL | 95059–198 | |
| Commercial assay or kit | SureBlue TMB Microwell Peroxidase Substrate | KPL | 95059–282 | |
| Commercial assay or kit | HBSS, without Calcium and Magnesium | Corning | 21022CV | |
| Commercial assay or kit | HBSS, with Calcium and Magnesium | Corning | 21023CV | |
| Commercial assay or kit | CleanCap Cas9 mRNA | TriLink | L-7206 | |
| Commercial assay or kit | MEGAshortscript T7 Transcription Kit | Ambion | AM1354 | |
| Commercial assay or kit | MEGAclear Transcription Clean-Up Kit | Ambion | AM1908 | |
| Commercial assay or kit | EDTA-free Protease Inhibitor Cocktail | Sigma-Aldrich | 11 836 170 001 | |
| Commercial assay or kit | ECL Western Blotting Substrate | Pierce | 32106 | |
| Commercial assay or kit | SuperScript III Reverse Transcriptase | Invitrogen | 18080044 | |
| Commercial assay or kit | StemPro Osteogenesis Differentiation Kit | Gibco | A1007201 | |
| Commercial assay or kit | StemPro Adipogenesis Differentiation Kit | Gibco | A1007001 | |
| Commercial assay or kit | StemPro Chondrogenesis Differentiation Kit | Gibco | A1007101 | |
| Commercial assay or kit | MicroVue DPD ELISA Kit | Quidel | 8007 | |
| Commercial assay or kit | MicroVue Creatinine Assay Kit | Quidel | 8009 | |
| Commercial assay or kit | Rat/Mouse PINP ELISA kit | Immunodiagnostic Systems | AC-33F1 | |
| Commercial assay or kit | Nuclear/Cytosol Fractionation Kit | Biovision | K266 | |
| Other | Fetal bovine serum | Sigma-Aldrich | F2442 | |
| Other | Penicillin Streptomycin 100x solution | HyClone | SV3001 | |
| Other | DMEM, low glucose | Gibco | 11885084 | |
| Other | DMEM, high glucose | Gibco | D5671 |