Table 3.
Conditions and reagents used for immunohistochemistry studies.
| Antibody specificity | Source | M/R (Clone)a | Antigen retrievalb | Working Dilution |
|---|---|---|---|---|
| PD-1 | Origene | M (UMAB199) | E (20min) | 1/500 |
| PD-L1 | Cell Signalling | M(405.9A11) | E (20min) | 1/100 |
| PD-L1 | Spring Bioscience | R (SP142) | E (20min) | 1/100 |
| HLA-I | AbCam | R (EP1395Y) | TC (10min) | 1/250 |
| HLA-II (DR) | ThermoFisher | M (LN-3) | TC (10min) | 1/300 |
| HLA-II (DQ) | AbCam | M (ab55158) | TC (10min) | 1/50 |
| CD68 | DAKO | M (1G12) | TC (10min) | 1/100 |
| Cytocheratin 8/18 | Ventana, | B22.1 B23.1 | TC (10min) | 1/1 |
| CD4 | Ventana | R (SP35) | E (10min) | 1/1 |
| CD8 | Ventana | R (SP57) | TC (10min) | 1/1 |
a M, mouse; R, rabbit.
b E, EDTA pH 8,0; TC, citrate buffer pH 6,0; in parenthesis, incubation time.