Abstract
Bacterial endophthalmitis is the most severe form of vision-threatening ocular infection. Mycoplasma pneumoniae is a primary respiratory pathogen that has been associated with several extrapulmonary manifestation, including ocular infection. In this work, we presented the microbiology diagnosis of an endophalmitis by M. pneumoniae using a novel A.R.I. WELL D-ONE® (CPM Scientifica, Italy) commercial micromethod in a Cuban pediatric patient. After positive M. pneumoniae confirmation, treatment with levofloxacin was initiated and successfully eliminated the ocular infection.
Keywords: Mycoplasma pneumoniae, A.R.I. WELL D-ONE, endophalmitis
Introduction
Bacterial endophthalmitis is the most severe form of vision-threatening ocular infection.1 Mycoplasma pneumoniae (MP) is a respiratory pathogen that could cause ocular infection as extrapulmonary manifestation.2 The diagnosis of MP infection is principally by PCR, but in Cuba, it is only possible at Mycoplasma Reference Laboratory from Pedro Kourí Tropical Medicine Institute (IPK). In this work, we presented a case of endophthalmitis by MP and the detection by a commercial kit A.R.I. WELL D-ONE® (CPM Scientifica, Italy), culture and PCR.
Case
A 2-month-old infant was attended at Juan Manuel Márquez University Pediatric Hospital (JMM) for an ocular infection in the right eye (RE). Clinical records showed that after delivery, the newborn was hospitalized at JMM for a severe neonatal sepsis, respiratory distress, and eyes opalescence. Clinical diagnosis of endogen endophthalmitis was done at ophthalmology service of JMM. Serial microbiological culture from blood and ocular swabs were negative and several syndromic antimicrobial treatments were used without improvement of ocular infection, including some intraocular antimicrobial injections.
At this time, the JMM treating medical team decided to perform the RE-vitrectomy with microbiological sampling of vitreous fluid (VF), because of the non-improvement of RE infection and the beginning of compromising of the left eye (LE). Bacteriological and mycological traditional culture for Gram-negatives, Gram-positives, anaerobic bacteria, and fungus were done with negative results. At the same time, the VF was inoculated in the A.R.I. kit and after 48 h, the kit was positive for MP (Figure 1). VF and the positive A.R.I. kit were sent to IPK for MP confirmation. MP qPCR for P1 gene was positive in the two samples. Glycolytic-mycoplasma typical culture in Friis medium3 was recorded at 24 h from the positive wells of the kit and cultures of VF showed grown 7 days after inoculation. The mycoplasma isolate was identified as MP by PCR.
Figure 1.
A.R.I. WELL D-ONE® kit: (A) un-inoculated (negative control) and (B) inoculated with vitreous fluid after 48 h of incubation.
Negative results by the A.R.I. WELL were recorded to Streptococcus pneumoniae, Streptococcus pyogenes, Strep-tococcus agalactiae, Haemophilus influenzae, Staphylococcus aureus, Mycoplasma hominis, Pseudomonas spp., and Candida albicans.
After positive MP confirmation, treatment with levofloxacin was initiated and successful elimination of the ocular infection of the LE was obtained after 14 days. At date, the boy is healthy, with vision diminution of the LE and no other clinical manifestation of MP as recorded by the treated physician.
Discussion
MP has been detected causing ocular infection but to our knowledge, this is the first report in Cuba and for a severe MP ocular infection with an eye loss outcome. Other internationally published investigation detects MP causing conjunctivitis, uveitis, and perineuritis, and some association was done with mycoplasma infections and these diseases.4,5
The present case showed that MP could be responsible for ocular infections in Cuba, and the A.R.I. WELL D-ONE® kit could be an alternative in the MP diagnosis in rare ocular infection. Future studies are necessary to know the real significance of MP as responsible for ocular infection in Cuba and the improvement of microbiological diagnosis with the introduction of new alternative accelerated micromethods.
Acknowledgments
We thank all the technicians of the Bacteriology-Mycology Department of IPK and Juan Manuel Marquez Pediatric Hospital who worked in the diagnostic and confirmation of the laboratory results.
Footnotes
Declaration of conflicting interests: The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
Ethical approval: Ethical approval to report this case was obtained from *PEDRO KOURI TROPICAL MEDICINE INSTITUTE—INSTITUTIONAL REVIEW BOARD (CEI-IPK 33-12).
Funding: The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: The present work was supported by the Pedro Kourí Tropical Medicine Institute – Cuba. BAMR and NMRP have received travel grant from CPM Scientifica to participate in scientific meeting.
Informed consent: Written informed consent was obtained from a legally authorized representative(s) for anonymized patient information to be published in this article.
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