Fig. 4.

Informative content (IC) of each amino acid position along the capsid, and analysis of the frequency of the NSyn mutations. (a) The analysis of the informative content was performed using overlapping windows of 10 amino acids sliding every 3 amino acids. IC values (in bits) range from 0 (maximum variability) to 4·32 (minimum variability). Each point (amino acid position) corresponds to the mean IC from all the sequences of a given group. The black line corresponds to the non-vaccinated group and the red line to the vaccinated group. Values have been normalized by the frequency of each substitution and the number of sequences per group. (b) Frequency (%) of the NSyn mutations detected in the non-vaccinated patients (n = 8; black dots) and vaccinated patients (n = 5; red dots); black dots are more common due to the higher n. The horizontal green line represents the 5% frequency; points over this line represent high-frequency mutations.