Fig. 3.

Evaluation of glutamate uptake in MSCs⁺ and MSCs. (A) [³H]glutamate uptake in MSCs⁺ and MSCs in the presence of increasing concentrations of non-labeled glutamate. (B) Inhibition of [³H]glutamate uptake mediated by the simultaneous application of DHK (300 μM) and TBOA (500 μM) in MSCs⁺. (C) Inhibition of [³H]glutamate uptake with both inhibitors applied individually to MSCs⁺ (***p < 0.001, n = 6). (D) [³H]glutamate uptake assay in HEK⁺ and HEK⁻ cells. (E) Selective inhibition of glutamate uptake mediated by the simultaneous application of DHK and TBOA in HEK⁺ cells, and (F) inhibition of glutamate uptake with both inhibitors administered individually to HEK⁺ cells (***p < 0.001, n = 6). Radioactive disintegrations per minute (DPM); protein (prot); dl-threo-beta-benzyloxyaspartate (TBOA); dihydrokainate (DHK); non-transfected MSCs (MSC⁻); transfected MSCs (MSC⁺).