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. 2018 Dec 21;8(1):6. doi: 10.3390/cells8010006

Figure 5.

Figure 5

Effects of DIFs on glucose consumption (uptake) by confluent 3T3-L1 fibroblasts. Confluent 3T3-L1 fibroblasts were incubated at 37 °C for at least 8 h in the presence of 0.2% EtOH (vehicle; Control) or 20 µM of one of the DIF derivatives. The glucose concentration in each incubation medium was measured and the approximate rate of glucose consumption was calculated relative to that in the Control medium; the rate of glucose consumption corresponds well with that of tritium-labeled 2-deoxy-glucose uptake promoted by DIF-1 [84]. The means and SD (bars) of three independent experiments (A) or triplicate experiments (B) are shown (adapted from Omata et al. [84] and Kubohara et al. [71], respectively). * p < 0.05 versus Control (by t-test; two-tailed, unpaired).